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A constitutive catabolite repression mutant of a recombinantSaccharomyces cerevisiae strain improves xylose consumption during fermentation

  • Industrial Microbiology
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Abstract

Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinantSaccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions.S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within theMIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in nitrogen-limited chemostat cultivations. This study shows that a constitutive catabolite repressed mutant could be used to enhance the xylose consumption rate even in the presence of high glucose in the fermentation medium. This could help in reducing fermentation time and cost in mixed sugar fermentation.

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Author notes

  1. Ricardo R. Cordero Otero

    Present address: Department of Biochemistry and Biotechnology, University Rovira i Virgili, Marcel li D. Str., E-43007, Tarragona, Spain

Authors and Affiliations

  1. Institute for Wine Biotechnology, Stellenbosch University, Private Bag X1, Matieland, 7600, Stellenbosch, ZA, South Africa

    Vasudevan Thanvanthri Gururajan, Isak S. Pretorius & Ricardo R. Cordero Otero

  2. Department of Applied Microbiology, Lund University, PO Box 124, 22100, Lund, SE, Sweden

    Marie-F. Gorwa-Grauslund & Bärbel Hahn-Hägerdal

  3. The Australian Wine Research Institute, Glen Osmond, PO Box 197, 5064, Adelaide, SA, Australia

    Isak S. Pretorius

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  1. Vasudevan Thanvanthri Gururajan
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Correspondence to Isak S. Pretorius.

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Thanvanthri Gururajan, V., Gorwa-Grauslund, MF., Hahn-Hägerdal, B. et al. A constitutive catabolite repression mutant of a recombinantSaccharomyces cerevisiae strain improves xylose consumption during fermentation. Ann. Microbiol. 57, 85–92 (2007). https://doi.org/10.1007/BF03175055

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