Abstract
Selectivelyl3C-labeled ubiquinone anion radicals in protic and aprotic solvents are investigated by EPR and ENDOR spectroscopy, yielding information about the effect of hydrogen bonds on the electronicg-tensor and the carbonyl carbon13C-hf tensors. Formation of the hydrogen bonds alter theg-tensor significantly to lower values and increases theA zz , component of thel3C-hf tensor. Both effects can be explained by electrostatic interactions between the positively charged hydrogen and the electrons at the carbonyl oxygen leading to a redistribution of charge and π-spin density. Two different hydrogen bonds were obtained for UQ −0 which are in agreement with the results of DFT (density functional theory) calculations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Lubitz W., Abresch E.C., Debus, R.J., Isaacson R.A., Okamura M.Y., Feher G.: Biochim. Biophys. Acta808, 464–469 (1985)
Feher G., Isaacson R.A., Okamura M.Y., Lubitz W.: Ser. Chem. Phys., vol.42, pp. 174–189 Berlin: Springer 1985.
Trumpower B.L. (ed.): Function of Quinones in Energy-Conserving Systems. New York: Academic Press 1982.
Lancaster C.R.D., Ermler U., Michel H. in: Anoxygenic Photosynthetic Bacteria (Blankenship R.E., Madigan M.T., Bauer C.E., eds.), pp. 503–526. Dordrecht: Kluwer 1995.
Brudler R., de Groot H.J.M., van Liemt W.B.S., Steggerda W.F., Esmeijer R., Gast P., Hoff A.J., Lugtenburg J., Gerwert K.: EMBO J.13, 5523–5530 (1994)
Breton J., Boullais C., Burie J.-R., Nabedryk E., Mioskowski C.: Biochemistry33, 14378–14386 (1994)
van den Brink J.S., Spoyalov A.P., Gast P., van Liemt W.B.S., Raap J., Lugtenburg J., Hoff A.J.: FEBS Lett.353, 273–276 (1994)
Isaacson R.A., Abresch E.C., Lendzian F., Boullais C., Paddock M., Mioskowski C., Lubitz W., Feher G. in: The Reaction Center of Photosynthetic Bacteria, Structure and Dynamics (Michel-Beyerle M.-E., ed.), pp. 353–367. Berlin: Springer 1996.
Kropacheva T.N., Raap J., Lugtenburg J., Hoff A.J. in: Photosynthesis: from Light to Biosphere (Mathis P., ed.), vol. I, pp. 527–530. Dordrecht: Kluwer 1995.
Samoilova R.I., Gritsan N.P., Hoff A.J., van Liemt W.B.S., Lugtenburg J., Spoyalov A.P., Tsvetkov Y.D.: J. Chem. Soc. Perkin Trans.2 1995, 2063–2068; Samoilova R.I., van Liemt W.B.S., Steggerda W.F., Lugtenburg J., Hoff A.J., Tyryshkin A.M., Gritsan N.P., Spoyalov A.P.: J. Chem. Soc. Perkin Trans. 21994, 609–614.
Lendzian F.: Ph. D. Thesis, Freie Universität Berlin, Berlin 1982.
Zweygart W., Thanner R., Lubitz W.: J. Magn. Reson.109A, 172–176 (1994)
Atherton N.M.: Principles of Electron Spin Resonance. Chichester: Ellis Horwood 1993.
Rieger P.H.: J. Magn. Reson.50, 485–489 (1982)
Fiege R.: Ph. D. Thesis, Technische Universität Berlin, Berlin 1997.
Lendzian F., Sahlin M., MacMillan F., Bittl R., Fiege R., Poetsch S., Sjöberg B.-M., Gräslund A., Lubitz W., Laßmann G.: J. Am. Chem. Soc.118, 8111–8120 (1996)
Lubitz W., Lendzian F. in: Biophysical Techniques in Photosynthesis (Amesz J., Hoff A.J., eds.), pp. 255–275. Dordrecht: Kluwer Academic Publishers 1996.
Stone A.J.: Proc. Roy. Soc. A London271, 424–434 (1963)
Burghaus O., Plato M., Rohrer M., Möbius K., MacMillan F., Lubitz W.: J. Phys. Chem.97, 7639–7647 (1993)
DMOL Release 95.0 Molecular Simulations Inc. 1995.
Perdrew J.P., Wang Y.: Phys. Rev. B45, 13244 (1992)
Becke A.D.: J. Chem. Phys.88, 2547–2553 (1988)
McConnel H.M., Strathdee J.: J. Mol. Phys.2, 129–138 (1959)
MacMillan F., Lendzian F., Lubitz W.: Magn. Reson. Chem.33, S81-S93 (1995)
McConnell HJ.: J. Phys. Chem.24, 764–766 (1956)
Heller C., McConnell H.M.: J. Chem. Phys.32, 1535–1539 (1960)
Freed J.H., Fraenckel G.K.: J. Chem. Phys.39, 326–348 (1963)
Freed J.H., Fraenckel G.K.: J. Chem. Phys.40, 1815–1829 (1964)
Kropacheva T.N., van Liemt W.B.S., Raap J., Lugtenburg J., Hoff A.J.: J. Phys. Chem.100, 10433–10442 (1996)
Karplus M., Fraenckel G.K.: J. Chem. Phys.35, 1312–1360 (1961)
Wertz J.E., Bolton J.R., Weil J.A.: Electron Spin Resonance. New York: McGraw-Hill 1994.
Burghaus O.: Ph. D. Thesis, Freie Universität Berlin, Berlin 1991.
Kirste B., Niethammer D., Kurreck H.: Appl. Magn. Reson.3, 1–18 (1992)
Mallikan R.S.: J. Chem. Phys.23, 1833–1848 (1955)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nimz, O., Lendzian, F., Boullais, C. et al. Influence of hydrogen bonds on the electronicg-tensor and13C-hyperfine tensors of13C-labeled ubiquinones — EPR and ENDOR study. Appl. Magn. Reson. 14, 255–274 (1998). https://doi.org/10.1007/BF03161893
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03161893