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Differential antioxidative responses of ascorbate-glutathione cycle enzymes and metabolites to chromium stress in green gram (Vigna radiata L. wilczek) leaves

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Abstract

Chromium-induced antioxidative responses of ascorbate-glutathione cycle enzymes and metabolites in green gram(Vigna radiata L. Wilczek) leaves were investigated in both dose and time-dependent manners. Rapid uptake of Cr was observed immediately after the start of treatment. Significant reduction was observed in leaf biomass under 300 µM Cr-treatment. Treatment with 300 µM Cr increases the content of hydrogen peroxide and Superoxide dismytase activity upto initial 96 h, and then gradually declined to the basal level. Ascorbate peroxidase and guaiacol peroxidase activities were low in 300 µM Cr-treated leaves during the first 96 h, but significantly increased therefore, suggesting that increased enzyme activities would be responsible for the removal of H2O2. Catalase activities were always suppressed under Cr stress. Contents of reduced ascorbate and dehydroascorbate were significantly decreased under 300 uM Cr-treatment. The reduced glutathione content decreased at early stages of Cr-treatment. However, it was restored to the normal level as in controls thereafter. In contrast, the glutathione disulphide content showed a progressive increase during the initial hours of Cr-treatment. The non-protein thiol content was shown to increase during the first several hours, but it declines at later stages. The present results demonstrate that Cr-induced oxidative stress is an important component of the plant’s reaction to toxic levels of Cr.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

Cr:

chromium

DHA:

dehydroascorbate

GPX:

guaiacol peroxidase

GSH:

reduced glutathione

CSSC:

glutathione disulphide

H2O2 :

hydrogen peroxide

NPT:

non-protein thiol

POX:

peroxidase

ROS:

reactive oxygen species

SOD:

Superoxide dismutase

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Authors and Affiliations

  1. Department of Plant Sciences, Centre of Potential in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, 625 021, Madurai, India

    Thirupathi Karuppanapandian, Pritam Bala Sinha & Kumariah Mamoharan

  2. Department of Biotechnology, Srimad Andavan Arts and Science College, 620 005, Thiruvanaikoil, Tiruchirappalli, India

    Abdul Majeeth Kamarul Haniya

Authors
  1. Thirupathi Karuppanapandian
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  2. Pritam Bala Sinha
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  3. Abdul Majeeth Kamarul Haniya
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  4. Kumariah Mamoharan
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Correspondence to Thirupathi Karuppanapandian.

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Karuppanapandian, T., Sinha, P.B., Haniya, A.M.K. et al. Differential antioxidative responses of ascorbate-glutathione cycle enzymes and metabolites to chromium stress in green gram (Vigna radiata L. wilczek) leaves. J. Plant Biol. 49, 440–447 (2006). https://doi.org/10.1007/BF03031124

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  • DOI: https://doi.org/10.1007/BF03031124

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