Abstract
Apoptosis, a type of physiological or active cell death, has been implicated as a mechanism underlying regression of the corpus luteum (CL) in the rat, bovine, rabbit and ovine ovary. Previousin vitro studies of cultured luteal cells have also provided evidence which suggests that reactive oxygen species play an important role in luteolysis in the rodent ovary. To further evaluate the potential role of oxidative stress in luteal cell demise, changes in the expression of several enzymes known to protect cells from oxidative stress were investigated using bovine CL collected from ovaries of non-pregnant (day 21 of the estrous cycle; regressed CL) and pregnant (day 21 of pregnancy; functional CL) animals. Biochemical analysis of genomic DNA extracted from these two pools of CL demonstrated the presence of extensive levels of internucleosomal DNA cleavage characteristic of cell death via apoptosis in regressed, but not in functional, CL. Northern blot analysis of total RNA indicated that functional CL expressed significantly higher levels of mRNA encoding secreted superoxide dismutase (SEC-SOD, 1.9 kb) and manganese-containing or mitochondrial SOD (Mn-SOD, multiple transcripts) as compared to regressed CL. Similarly, levels of mRNA encoding catalase (2.1 kb), an enzyme responsible for detoxification of peroxides to water, were significantly higher in functional versus regressed CL. From these data, we conclude that a decline in expression of specific oxidative response genes occurs during luteolysis, and that maintained expression of these genes in the CL during pregnancy may prevent oxidative damage and delay regression.
Similar content being viewed by others
References
Chomczynski, P. & Sacchi, N. (1987).Anal. Biochem.,162, 156–159.
Dharmarajan, A.M., Goodman, S.B., Tilly, K.I. & Tilly, J.L. (1994).Endocr. J. (Endocrine),2, 295–303.
Feinberg, A.P. & Vogelstein, B. (1983).Anal. Biochem.,132, 6–13.
Gatzuli, E., Aten, R. & Behrman, H.R. (1991).Endocrinology,128, 2253–2258.
Gross-Bellard, M., Oudet, P. & Chambon, P. (1973).Eur. J. Biochem.,36, 32–38.
Hockenberry, D.M., Oltvai, Z.N., Yin, X.-M., Milliman, C.L. & Korsmeyer, S.J. (1993).Cell,75, 241–251.
Juengal, J.L., Garverick, H.A., Johnson, A.L., Youngquist, R.S. & Smith, M.F. (1993).Endocrinology,132, 249–254.
Kerr, J.R.F., Wyllie, A.H. & Currie, A.R. (1972).Br. J. Cancer,26, 239–257.
Keyes, P.L. & Wiltbank, M.C. (1988).Ann. Rev. Physiol.,50, 465–482.
Laloraya, M., Pradeep, K.G. & Laloraya, M.M. (1988).Biochem. Biophys. Res. Commun.,157, 146–153.
McConkey, D.J., Hartzell, P., Nicotera, P. & Orrenius, S. (1989).Toxicol. Lett.,42, 123–130.
Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K. & Green, M.R. (1984).Nucl. Acids Res.,12, 7035–7056.
Musicki, B., Aten, R.F. & Behrman, H.R. (1994).Endocrinology,134, 588–595.
Nett, T.M., McClellan, M.C. & Niswender, G.D. (1976).Biol. Reprod.,15, 66–78.
Niswender, G.D., Schwall, R.H., Farin, C.E. & Sawyer, H.R. (1985).Rec. Prog. Norm. Res.,41, 101–151.
Orlicky, D.J., Fisher, L., Dunscomb, N. & Miller, G.J. (1992).Prostaglandins, Leukotrienes and Essential Fatty Acids,46, 223–229.
Pate, J.L. (1994).J. Anim. Sci.,72, 1884–1890.
Pate, J.L. & Condon, W.A. (1984).Biol. Reprod.,31, 427–435.
Riley, J.C.M. & Behrman, H.R. (1991).Endocrinology,128, 1749–1753.
Rosen, D.R., Siddique, T., Patterson, D., Figlewicz, D.A., Sapp, P., Hentati, A., Donaldson, D., Goto, J., O’Regan, J.P. & Deng, H.X. (1993).Nature,362, 59–62.
Rueda, B.R., Naivar, K.A., George, E.M., Austin, K.J., Francis, H. & Hansen, T.R. (1993).J. Interferon Res.,13, 303–309.
Rueda, B.R., Tilly, K.I., Hoyer, P.B., Hansen, T.R. & Tilly, J.L. (1994). Proceedings from the Xth Ovarian Workshop, Serono Symposia, USA, University of Michigan, Ann Arbor, p. 27 (Abstract).
Rueda, B.R., Wegner, J.A., Marion, S.L., Wahlen, D.E. & Hoyer, P.B. (1995).Biol. Reprod.,52, 305–312.
Sawada, M. & Carlson, J.C. (1991).Endocrinology,128, 2992–2998.
Sawada, M. & Carlson, J.C. (1994).Endocrinology,135, 1645–1650.
Sawyer, H.R., Niswender, K.D., Braden, T.D. & Niswender, G.D. (1990).Dom. Anim. Endocrinol.,7, 229–238.
Tilly, J.L. (1993).Endocr. J. (Endocrine),1, 67–72.
Tilly, J.L. & Hsueh, A.J.W. (1993).J. Cell. Physiol.,154, 519–526.
Tilly, J.L., LaPolt, P.S. & Hsueh, A.J.W. (1992a).Endocrinology,130, 1296–1302.
Tilly, J.L., Kowalski, K.I., Schomberg, D.W. & Hsueh, A.J.W. (1992b).Endocrinology,131, 1670–1676.
Tilly, J.L. & Tilly, K.I. (1995).Endocrinology,136, 242–252.
Tilly, J.L., Tilly, K.I., Kenton, M.L. & Johnson, A.L., (1995).Endocrinology,136, 232–241.
Wong, G.H.W., Elwell, J.H., Oberly, L.W. & Goeddel, D.V. (1989).Cell,58, 923–931.
Wyllie, A.H. (1980).Nature,284, 555–556.
Yu, B.P. (1994).Physiol. Rev.,74, 139–162.
Author information
Authors and Affiliations
Additional information
This study was supported by grants from the National Institute of Child Health and Human Development (NIH R01-HD26778 and R01-HD00907 to P.B.H.; NIH R55-HD3H88 to J.L.T.), and grants from The Johns Hopkins University Faculty Development Program and the American Federation for Aging Research (to J.L.T.)
Rights and permissions
About this article
Cite this article
Rueda, B.R., Tilly, K.I., Hansen, T.R. et al. Expression of superoxide dismutase, catalase and glutathione peroxidase in the bovine corpus luteum: evidence supporting a role for oxidative stress in luteolysis. Endocr 3, 227–232 (1995). https://doi.org/10.1007/BF02994448
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02994448