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Reduction of chromium(VI) in Chinese hamster V-79 cells

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Abstract

The cellular reduction of chromate(VI) was studied by electron spin resonance spectrometry. Incubation of Chinese hamster V-79 cells with Na2CrO4 resulted in the formation of both chromium(V) and chromium(III) complex in a manner dependent on time (30 min-2 h) and concentration (50–500 μM). Following removal of extracellular chromate, the level of chromium(V) complex decreased quickly during the first hour but more slowly for the next hour, whereas the level of chromium(III) remained unchanged, indicating that chromium(III) is the ultimate ion of this metal in cells. Alkaline elution studies demonstrated that treatment of cells with Na2CrO4 induced DNA single-strand breaks that decreased quickly and DNA-protein crosslinks that persisted for 2 h after removal of this metal. These results suggest that the cellular levels of chromium(V) and chromium(III) may be associated with the formation of DNA damage induced by chromium (VI).

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Authors and Affiliations

  1. Department of Medical Biochemistry, Kurume University School of Medicine, 67 Asahi-machi, 830, Kurume, Japan

    Masayasu Sugiyama, Katsuyuki Tsuzuki, Toshihiro Hidaka & Ryohei Ogura

  2. Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-01, Jonanku, Fukuoka, Japan

    Magobei Yamamoto

Authors
  1. Masayasu Sugiyama
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  2. Katsuyuki Tsuzuki
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  3. Toshihiro Hidaka
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  4. Ryohei Ogura
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  5. Magobei Yamamoto
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Sugiyama, M., Tsuzuki, K., Hidaka, T. et al. Reduction of chromium(VI) in Chinese hamster V-79 cells. Biol Trace Elem Res 30, 1–8 (1991). https://doi.org/10.1007/BF02990337

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  • DOI: https://doi.org/10.1007/BF02990337

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