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Isozyme and amplified fragment length polymorphisms fromCephalosporium maydis in Egypt

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Abstract

Isoenzyme and amplified fragment length polymorphisms (AFLP) variation within a set of 48 isolates ofCephalosporium maydis was characterized. These isolates included ten cultures that have served as standards in the Egyptian maize resistance breeding program and 38 additional strains collected from 11 governates in Egypt during the 1997 growing season. Eight isozymes also were tested, but only five (acid phosphatase, fumerase, gtucose 4-phosphate isomerase, isocitrate dehydrogenase, and malate dehydrogenase) produced identifiable bands and all five of these enzymes were monomorphic. Sixty-eight AFLP primer-pair combinations were used and 865 bands were scored, of which 288 (33%) were polymorphic and could be used to discriminate four distinct subgroups, or lineages. Representatives from only two of the four lineages are included in the set of ten strains that has been used to challenge new lines in the Egyptian maize breeding program. From among these 68 primer-pair combinations, we identified a set of four AFLP primer-pairs that were strongly correlated (Pearson‘sr > 0.85) with the full data set that can be used as markers to determine the distribution of these lineages and to identify new lineages in field populations.

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References

  1. Abel-el-Rahim, M.F., Sabet, K.A., El-Shafey, H.A. and El-Assiuty, E.M. (1982) Chemical control of the late-wilt disease of maize caused byCephalosporium maydis. Agric. Res. Rev., Egypt 60:31 -49.

    Google Scholar 

  2. Anderson, J.B. and Kohn, L.M. (1995) Clonality in soilbome plant-pathogenic fungi.Annu. Rev. Phytopathol. 33:369–391.

    Article  CAS  PubMed  Google Scholar 

  3. Casela, C.R., Ferreira, A.S., Zeller, K.A. and Levy, M (1995) Pathotype variation in the sorghum anthracnose fungus: a phylogenetic perspective for resistance breeding.in: Leslie, J.F. and Frederiksen, R.A. [Eds.] Disease Analysis through Genetics and Biotechnology: Interdisciplinary Bridges to Improved Sorghum and Millet Crops.pp. 257–2766. Iowa State University Press, Ames, Iowa, USA.

    Google Scholar 

  4. Chen, W.D. (1994) Vegetative compatibility groups ofVerticillium dahliae from ornamental woody plants.Phytopathology 84:214–219.

    Article  Google Scholar 

  5. Chen, X., Line, R.F. and Leung, H. (1993) Relationship between virulence variation and DNA polymorphism inPuccinia striiformis. Phytopathology 83:1489–1497.

    Article  CAS  Google Scholar 

  6. Correll, J.C, Klittich, C.J.R. and Leslie, J.F. (1987) Nitrate non-utilizing mutants ofFusarium oxysporum and their use in vegetative compatibility tests.Phytopathology 77:1640–1646.

    Article  Google Scholar 

  7. Dobinson, K.F., Patterson, N.A., White, G.J. and Grant, S. (1998) DNA fingerprinting and vegetative compatibility analysis indicate multiple origins forVerticillium dahliae race 2 tomato isolates from Ontario, Canada.Mycol. Res. 102:1089–1095.

    Article  CAS  Google Scholar 

  8. Drenth, A., Tas, I.C.Q. and Govers, F. (1994) DNA fingerprinting uncovers a new sexually reproducing population ofPhytophthora infestons in the Netherlands.Eur. J. Plant Pathol. 100:97–107.

    Article  Google Scholar 

  9. El-Assiuty, E.M., El-Shafey, H.A., Ismael, A.-S., Fahmy, Z.M. and El-Abedein, A.Z. (1998) Pathogenic variation inCephalosporium maydis. Phytopathology 88:S25.

    Google Scholar 

  10. El-Assiuty, E.M., Ismael, A.M., Zeller, K.A. and Leslie, J.F. (1999) Relative colonization ability of greenhouse-grown maize by four lineages ofCephalosporium maydis from Egypt.Phytopathology 89:S23.

    Google Scholar 

  11. Elias, K.S., Zamir, D., Lictman-Pleban, T. and Katan, T. (1993) Population structure ofFusarium oxysporum f.sp.lycopersicr: restriction fragment length polymorphisms provide genetic evidence that vegetative compatibility group is an indicator of evolutionary origin.Molec. Plant-Microbe Interact. 6:565–572.

    CAS  Google Scholar 

  12. El-ltriby, H.A., Khamis, M.N., El-Demerdash, R.M. and El-Shafey, H.A. (1984) Inheritance of resistance to late wilt(Cephalosporium maydis) in maize.Proc. 2nd Mediterranean Conf. on Genetics (Cairo, Egypt), pp. 29–44.

  13. El-Shafey, H.A., Abd-el-Rahim, M.F., Abd-el-Azim, O.Z. and Abd-el-Hamid, M.S. (1976) Carry-over of maize stalk-rot fungi in seed.Agric. Res. Rev., Egypt 54:29–42.

    Google Scholar 

  14. El-Shafey, H.A., El-Shorbagy, F.A., Khalil, I.I. and El-Assiuty, E.M. (1988) Additional sources of resistance to the late-wilt disease of maize caused byCephalosporium maydis.Agric. Res. Rev., Egypt 66:221–230.

    Google Scholar 

  15. El-Shafey, H.A., Mahmoud, S.A.Z., El-Brolousy, M., El-Azhary, T.M. and Ibrahim, T.F. (1990) Biological control of maize late-wilt disease by inoculation withBacillus subtilis and manuring.Egypt. J. Appl. Sci. 5:182–191.

    Google Scholar 

  16. Gonzalez, M., Rodriguez, M.E.Z., Jacabo, J.L., Hernandez, F., Acosta, J., Martinez, O. and Simpson, J. (1998) Characterization of Mexican isolates ofColletotrichum lindemuthianum by using differential cultivars and molecular markers.Phytopathology 88:292–299.

    Article  PubMed  CAS  Google Scholar 

  17. Hill, M., Witsenboer, H., Zabeau, M., Vos, P., Kesseli, R. and Michelmore, R. (1996) PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships inLactuca spp.Theor. Appl. Genet. 93:1202–1210.

    Article  CAS  Google Scholar 

  18. Huss, M.J., Campbell, C.L., Jennings, D.B. and Leslie, J.F. (1996) Isozyme variation in theGibberella fujikuroi species complex(Fusarium sectionLiseola).Appl. Environ. Microbiol. 62:3750–3756.

    PubMed  CAS  Google Scholar 

  19. Jacobson, K.M., Miller, O.K., Jr. and Turner, B.J. (1993) Randomly amplified polymorphic DNA markers are superior to somatic incompatibility tests for discriminating genotypes in natural populations of the ectomycorrhizal fungusSuillus granulatus.Proc. Natl. Acad. Sci. USA 90:9159–9163.

    Article  PubMed  CAS  Google Scholar 

  20. Janssen, P., Coopman, R., Huys, G., Swings, J., Bleeker, M., Vos, P., Zabeau, M. and Keraters, K. (1996) Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy.Microbiology 142:1881–1893.

    PubMed  CAS  Google Scholar 

  21. Keréyi, Z., Zeller, K., Hornok, L. and Leslie, J.F. (1999) Standardization of mating-type terminology in theGibberella fujikuroi species complex.Appl. Environ. Microbiol. 65:4071–4076.

    Google Scholar 

  22. Kistler, H.C., Momol, E.A. and Benny, U. (1991) Repetitive genomic sequences for determining relatedness among strains ofFusarium oxysporum.Phytopathology 81:331–336.

    Article  CAS  Google Scholar 

  23. Leslie, J.F. (1993) Fungal vegetative compatibility.Annu. Rev. Phytopathol. 31:127–150.

    Article  PubMed  CAS  Google Scholar 

  24. Levy, M., Correa-Victoria, F.J., Zeigler, R.S., Xu, S. and Hamer, J.E. (1993) Genetic diversity of the rice blast fungus in a disease nursery in Colombia.Phytopathology 83:1427–1433.

    Article  CAS  Google Scholar 

  25. Majer, D., Mithen, R., Lewis, B., Vos, P. and Oliver, R.P. (1996) The use of AFLP fingerprinting for the detection of genetic variation in fungi.Mycol. Res. 100:1107–1111.

    Article  CAS  Google Scholar 

  26. Meijer, G., Megnegneau, B. and Linders, E.G.A. (1994) Variability for isozyme, vegetative compatibility and RAPD markers in natural populations ofPhomopsis subordinaria.Mycol. Res. 98:267–276.

    CAS  Google Scholar 

  27. Milgroom, M.G. (1996) Recombination and the multilocus structure of fungal populations.Annu. Rev. Phytopathol. 34:457–477.

    Article  PubMed  CAS  Google Scholar 

  28. Milgroom, M.G., Lipari, E.S. and Powell, W.A. (1992) DNA fingerprinting and analysis of population structure in the chestnut blight fungusCryphonectria parasitica.Genetics 131:297–306.

    PubMed  CAS  Google Scholar 

  29. Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA.Nucleic Acids Res. 8:4321–4325.

    Article  PubMed  CAS  Google Scholar 

  30. Sabet, K.A., Samra, A.S. and Khalil, I.K. (1966) Variation inCephalosporium maydis, the cause of the late wilt disease,in: Samra, A.S. and Sabet, K.A. [Eds.] Investigations on Stalk-Rot Diseases of Maize in U.A.R. pp. 46–93. Ministry of Agriculture, Government Printing Offices, Cairo, Egypt.

    Google Scholar 

  31. Sabet, K.A., Samra, A.S. and Mansour, I.M. (1966) Late-wilt of maize and a study of the causal organism.in: Samra, A.S. and Sabet, K.A. [Eds.] Investigations on Stalk-Rot Diseases of Maize in U.A.R. pp. 8-45. Ministry of Agriculture, Government Printing Offices, Cairo, Egypt.

    Google Scholar 

  32. Sabet, K.A., Zaher, A.M., Samra, A.S. and Mansour, I.M. (1970) Pathogenic behavior ofCephalosporium maydis andC. acremonium.Ann. Appl. Biol. 66:257–263.

    Article  Google Scholar 

  33. Samra, A.S. and Sabet, K.A. (1966) An introductory note,in: Samra, A.S. and Sabet, K.A. [Eds.] Investigations on Stalk-Rot Diseases of Maize in U.A.R. pp. 1–7. Ministry of Agriculture, Government Printing Offices, Cairo, Egypt.

    Google Scholar 

  34. Samra, A.S., Sabet, K.A. and Abd-el-Rahim, M.F. (1966) Seed transmission of stalk-rot fungi and effect of seed treatment,in: Samra, A.S. and Sabet, K.A. [Eds.] Investigations on Stalk-Rot Diseases of Maize in U.A.R. pp. 94–116. Ministry of Agriculture, Government Printing Offices, Cairo, Egypt.

    Google Scholar 

  35. Samra, A.S., Sabet, K.A. and Hingorani, M.K. (1962) A new wilt disease of maize in Egypt.Plant Dis. Rep. 46:481–483.

    Google Scholar 

  36. Samra, A.S., Sabet, K.A. and Hingorani, M.K. (1963) Late wilt disease of maize caused byCephalosporium maydis.Phytopathology 53:402–406.

    Google Scholar 

  37. Samra, A.S., Sabet, K. A., Kamel, M. and Abd-el-Rahmin, M.F. (1971) Further Studies on the Effect of Field Conditions and Cultural Practices on Infection with Stalk-rot Complex of Maize. Arab Republic of Egypt, Min. of Agriculture, Plant Protection Dept., Bull. no. 2.

  38. Shehata, A.H. and Salem, A.M. (1972) Genetic analysis of resistance to late-wilt of maize caused byCepluilosporium maydis.Sabrao. J. 4:1–5.

    Google Scholar 

  39. Sicard, D., Michalikis, Y., Dron, M. and Neema, C. (1997) Genetic diversity and pathogenic variation ofColletotrichum lindemuthianum in three centers of diversity of its host,Phaseolus vulgaris.Phytopathology 87:807–813.

    Article  PubMed  CAS  Google Scholar 

  40. Spooner, D.M., Tivang, J., Nienhuis, J., Miller, J.T., Douches, D.S. and Contreras, M.A. (1996) Comparison of four molecular markers in measuring relationships among the wild potato relativesSolarium sectionEtuberosum (subgenusPotatoe).Theor. Appl. Genet. 92:532–540.

    Article  CAS  Google Scholar 

  41. Strausbaugh, CA., Schroth, M.N., Weinhold, A.R. and Hancock, J.G. (1992) Assessment of vegetative compatibility and virulence ofVerticillium dahliae isolates from Idaho potatoes and tester strains.Phytopathology 82:61–68.

    Article  Google Scholar 

  42. Swofford, D.L. (1998)PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0*. Sinauer Associates, Sunderland, MA, USA.

    Google Scholar 

  43. Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Homes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. (1995) AFLP: A new technique for DNA fingerprinting.Nucleic Acids Res. 23:4407–4414.

    Article  PubMed  CAS  Google Scholar 

  44. Zeigler, R.S., Tohme, J., Nelson, R.J., Levy, M. and Correa-Victoria, F. (1994) Lineage exclusion: A proposal for linking blast population analysis to resistance breeding.Proc. Int. Symp. on Rice Blast Disease (International Rice Research Institute, Los Bailos, the Philippines), pp. 267–292.

    Google Scholar 

  45. Zeigler, R.S., Cuoc, L.X., Scott, R.P., Bernardo, M.A., Chen, D.H., Valent, B. and Nelson, R.J. (1995) The relationship between lineage and virulence inPyricularia grisea in the Philippines.Phytopathology 85:443–451.

    Article  Google Scholar 

  46. Zeller, K.A., Jurgenson, J.E. and Leslie, J.F. (1998) Genetic variation inCephalospoiium inaydis from Egypt.Phytopathology 88:S103.

    Google Scholar 

  47. Zeller, K.A., Jurgenson, J.E. and Leslie, J.F. (1999) AFLP markers reveal genetic variation in Egyptian populations ofCephalospoiium maydis.Fungal Genet. Newsl. 46(suppl):94.

    Google Scholar 

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Authors and Affiliations

  1. Dept. of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, 66506-5502, KS, USA

    Kurt A. Zeller & John F. Leslie

  2. Dept. of Biology, University of Northern Iowa, Cedar Falls, 50614, IA, USA

    James E. Jurgenson

  3. Maize and Sorghum Diseases Section, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt

    Elhamy M. El-Assiuty

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  1. Kurt A. Zeller
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  2. James E. Jurgenson
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  3. Elhamy M. El-Assiuty
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  4. John F. Leslie
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Correspondence to John F. Leslie.

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Zeller, K.A., Jurgenson, J.E., El-Assiuty, E.M. et al. Isozyme and amplified fragment length polymorphisms fromCephalosporium maydis in Egypt. Phytoparasitica 28, 121–130 (2000). https://doi.org/10.1007/BF02981741

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  • DOI: https://doi.org/10.1007/BF02981741

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