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Induction of peroxidase as a disease resistance response in resistant (Hibiscus trionum) and susceptible (Althea armeniaca) species in the family malvaceae

  • Phytopathology
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Abstract

The pathogen-induced biosynthesis of peroxidase (PO) in resistant and susceptible wild species of Malvaceae was studied. Specifically,Hibiscus trionum was found to be resistant to the wilt pathogenVerticillium dahliae, andAlthea armeniaca was susceptible. After inoculation withV. dahliae, PO activity increased more rapidly in the resistant plant: increased activity inH. trionum was noted within 1 h after inoculation, remained high for 5 h, and decreased after 18 h. After 5 days, the levels did not differ from the control. In the susceptibleA. armeniaca, PO activity did not differ statistically from the control even after 18 h, but was significantly higher after 5 days. Electrophoretic analysis of inoculated tissue extracts showed the occurrence of two new isoforms inh. trionum after inoculation; new isoforms did not occur inA. armeniaca. A chitin affinity chromatography column showed the presence of a chitin-binding peroxidase isozyme in healthyH. trionum that increased dramatically after inoculation. Two chitin-binding peroxidase isozymes were observed in healthyA. armeniaca: one (R f 0.26) did not increase and the other (R f 0.89) evinced only a small increase after inoculation.

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Authors and Affiliations

  1. A.S. Sadykov Institute of Bioorganic Chemistry, Academy of Science Uzbekistan, Tashkent, Uzbekistan

    Zamira Golubenko, Alik Akhunov, Nigora Khashimova, Yuliana Beresneva, Elmira Mustakimova, Fazil Ibragimov & Nigora Abdurashidova

  2. U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, College Station, TX, USA

    Robert Stipanovic

Authors
  1. Zamira Golubenko
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  2. Alik Akhunov
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  3. Nigora Khashimova
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  4. Yuliana Beresneva
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  5. Elmira Mustakimova
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  6. Fazil Ibragimov
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  7. Nigora Abdurashidova
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Correspondence to Zamira Golubenko.

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http://www.phytoparasitica.org posting Aug. 5, 2007.

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Golubenko, Z., Akhunov, A., Khashimova, N. et al. Induction of peroxidase as a disease resistance response in resistant (Hibiscus trionum) and susceptible (Althea armeniaca) species in the family malvaceae. Phytoparasitica 35, 401–413 (2007). https://doi.org/10.1007/BF02980704

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  • DOI: https://doi.org/10.1007/BF02980704

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