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Biodetoxification of coumaphos insecticide using immobilizedEscherichia coli expressing organophosphorus hydrolase enzyme on cell surface

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Abstract

Recently, we reported an improved technology for the degradation of organophosphate nerve agents using whole cells of genetically engineeredEscherichia coli that anchored and displayed the enzyme organophosphorus hydrolase on the cell surface. In this paper we report the immobilization of these cells on highly porous sintered glass beads and the subsequent application of the immobilized cell in a continuous-flow packed bed bioreactor for the biodetoxification of a widely used insecticide, coumaphos.

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Authors and Affiliations

  1. Department of Chemical and Environmental Engineering, University of California, 92521, Riverside, CA, USA

    Wilfred Chen & Ashok Mulchandani

  2. Pesticide Chemistry Department, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt

    Ayman H. Mansee

Authors
  1. Ayman H. Mansee
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  2. Wilfred Chen
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  3. Ashok Mulchandani
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Correspondence to Ashok Mulchandani.

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Mansee, A.H., Chen, W. & Mulchandani, A. Biodetoxification of coumaphos insecticide using immobilizedEscherichia coli expressing organophosphorus hydrolase enzyme on cell surface. Biotechnol. Bioprocess Eng. 5, 436–440 (2000). https://doi.org/10.1007/BF02931944

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  • DOI: https://doi.org/10.1007/BF02931944

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