Abstract
The red yeastRhodotorula mucilaginosa strain CRUB 0138 (previously identified asR. lactosa) was isolated from a high-altitude Patagonian Lake Toncek (1700 m a.s.l.), and assigned withmucilaginosa species. Its biochemical, physiological and molecular features were assessed and compared toR. mucilaginosa PYCC 5166 type strain using a polyphasic approach; in addition, biomass and carotenoid pigment production at different C/N ratios were determined in an incubator shaker. Phenetic characterization by means of 70 current physiological tests including assimilation of aldaric acids and aromatic compounds, and also the ability to grow with amino acids as sole carbon sources, was carried out. According to numerical taxonomy calculations, similarity indexes betweenR. mucilaginosa CRUB 0138 and PYCC 5166 type strain were 0.86 and 0.77, corresponding to a complete set of physiological tests and MSP-PCR (Mini/Micro Satellite Primed PCR; (GTG)5, M13 and (GAC)5 primers were employed) fingerprinting. Killer activity against 2 native strains,Rhodosporidium kratochvilovae andR. mucilaginosa was detected. Maximum biomass-glucose conversion efficiency (87%) and maximum carotenoid yield (2.32 mg/L) were obtained at C/N=5 in culture medium containing 10 and 40 g/L glucose, respectively. Different C/N ratios did not influence carotenoid pigment production but low C/N enhanced biomass yield.
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Libkind, D., Brizzio, S. & van Broock, M. Rhodotorula mucilaginosa, a carotenoid producing yeast strain from a patagonian high-altitude lake. Folia Microbiol 49, 19–25 (2004). https://doi.org/10.1007/BF02931640
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DOI: https://doi.org/10.1007/BF02931640