Summary
Epithelial cell islets in primary monolayer cultures of human breast biopsies were characterized by combined immuno-, enzyme- and DNA cytochemistry as well as by analysis of attachment-, spread- and growth patterns. For cultivation we used expiants from reduction mammoplasties, benign lesions, primary carcinomas and metastases. Milk fat globule membrane antigen (MFGM-A) was detected with a monoclonal antibody, and the tetrazolium reaction for glucose 6-phosphate dehydrogenase (G6PDH) as well as DNA content of the cultured cells were quantified. Spreading and growth of individual islets were studied by image analysis.
Fibroblast-like cells did not express MFGM-A, and whereas epithelial (MFGM-A positive) cell islets of normal and benign origin showed cells with no or low G6PDH reaction, respectively, the majority of epithelial cell islets from 11 out of 21 carcinomas showed strong reaction. Cell islets with strong G6PDH reaction were sometimes hyperdiploid. Moreover, whereas cell islets with no or low reaction from both benign lesions and carcinomas readily attached and spread in a serum-free medium and showed population doubling times of 30 to 110 h, cell islets with strong reaction from carcinomas and metastatic lesions required serum for attachment and their growth rate was too low to be determined.
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Abbreviations
- CDM:
-
chemically defined medium
- G6PDH:
-
glucose 6-phosphate dehydrogenase
- G6PDH-re(+ +):
-
strong G6PDH reaction
- G6PDH-re(+):
-
moderate G6PDH reaction
- G6PDH-re(-):
-
no G6PDH reaction
- MFGM-A:
-
milk-fat gobule membrane antigen
- PDT:
-
population doubling time
- SSM:
-
serum-supplemented medium
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Petersen, O.W., Høyer, P.E., Hilgers, J. et al. Characterization of epithelial cell islets in primary monolayer cultures of human breast carcinomas by the tetrazolium reaction for glucose 6-phosphate dehydrogenase. Virchows Archiv B Cell Pathol 50, 27–42 (1986). https://doi.org/10.1007/BF02889888
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DOI: https://doi.org/10.1007/BF02889888