Abstract
One EST N27741 with high expression in normal adult nasopharynx tissues but low expression in adult poorly differentiated squamous nasopharyngeal carcinoma has been selected out by the high-density cDNA array expression profiling technique. The differential expression has been confirmed by RT-PCR. One novel gene of 1096 bp has been cloned based on this EST. Bioinformatics analysis found that the new gene sequence contains a whole reading frame encoding 256 amino acids. There is a stop codon TAA in front of the 5′ end start codon, and a tailing signal AATAAA and poly A tail at the 3′ end. There is no homologous known gene found after searching by blasting this sequence to non-redundancy nucleotide database. Therefore it is considered a novel gene related to nasopharyngeal carcinoma.
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He, Z., Xie, L., Xu, L. et al. Cloning of a novel gene associated with human nasopharyngeal carcinoma. Chin. Sci. Bull. 45, 2267–2272 (2000). https://doi.org/10.1007/BF02886366
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DOI: https://doi.org/10.1007/BF02886366