Skip to main content
Log in

Method for estimation of acid phosphatase in woodland soils

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

A new modification was proposed for the estimation of acid phosphatase in soils, using the assay of released 4-nitrophenol. The procedure is simple and it is adapted to ecological conditions in the temperate zone. The technique is applicable to work with soils containing humus at different pH values, different amounts of clay-size particles and featuring different biological activities. The method was tested using six samples of steppe and woodland soils distinguished by different properties and was found to be suitable for the purpose.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Brams W.H., McLaren A.D.: Phosphatase reactions in columns of soil.Soil Biol. Biochem. 6, 183–189 (1974).

    Article  CAS  Google Scholar 

  • Cervelli S., Nannipieri P., Ceccanti B., Sequi P.: Michaelis constant of soil acid phosphatase.Soil Biol. Biochem. 5, 841–845 (1973).

    Article  CAS  Google Scholar 

  • Dick W.S., Tabatabai M.A.: Hydrolysis of organic and inorganic phosphorus compounds added to soils.Geogerma 21, 175–182 (1978).

    CAS  Google Scholar 

  • Drobníková V.: Factors influencing the determination of phosphatases in soil.Folia Microbiol. 6, 260–267 (1961).

    Google Scholar 

  • Gerritse R.G., van Dijk H.: Determination of phosphatase activities of soils and animal wastes.Soil Biol. Biochem. 10, 545–551 (1978).

    Article  CAS  Google Scholar 

  • Harrison A.F.: Variation of four phosphatase properties in woodland soils.Soil Biol. Biochem. 11, 393–403 (1979).

    Article  CAS  Google Scholar 

  • Harrison A.F.: Relationship between intensity of phosphatase activity and physicochemical properties in woodland soils.Soil Biol. Biochem. 15, 93–99 (1983).

    Article  CAS  Google Scholar 

  • Harrison A.F., Pearce T.: Seasonal variation of phosphatase activity in woodland soils.Soil Biol. Biochem. 11, 405–410 (1979).

    Article  CAS  Google Scholar 

  • Ho I.: Acid phosphatase activity in forest soils.Forest Sci. 25, 567–568 (1979).

    Google Scholar 

  • Hoffman G.: Eine photometrische Methode zur Bestimmung der Phosphatase-Aktivität im Boden.Zeitschr. Pfl. Bodenk. 118, 161–172 (1968).

    Article  Google Scholar 

  • Jirkovský R., Tržil J.:Chemical Tables for Laboratory Use. (In Czech) Práce, Prague 1973.

    Google Scholar 

  • Khaziev F.C.:Systematic-ecological Analysis of the Fermentative Activity in Soils. (In Russian), Nauka, Moscow 1982.

    Google Scholar 

  • Kramer N., Erdeli G.: Use of the method of phosphatase activity estimation in agrochemical conditions. (In Russian)Pochvovedenie 9, 99–102 (1959).

    Google Scholar 

  • Malcolm R.E.: Assessment of phosphatase activity in soils.Soil Biol. Biochem. 15, 403–408 (1983).

    Article  CAS  Google Scholar 

  • Nikolsky B.P. (Ed.):The Chemist's Manual III. (In Russian) Khimiya, Moscow-Leningrad 1964.

    Google Scholar 

  • Pang P.C.K., Kolenko H.: Phosphomonoesterase activity in forest soils.Soil Biol Biochem. 18, 35–39 (1986).

    Article  CAS  Google Scholar 

  • Ramírez-Martínez J.A.: Organic phosphorus mineralization and phosphatase activity in soils.Folia Microbiol. 13, 161–174 (1968).

    Article  Google Scholar 

  • Skujinš J.J., Braal L., McLaren A.D.: Characterization of phosphatase in terrestrial soil sterilized with an electron beam.Enzymologia 25, 125–133 (1962).

    Google Scholar 

  • Sommer L.:Analytical Characteristics of Inorganic Ions and Substances (textbook). (In Czech) University J.E. Purkyně, Brno 1975.

    Google Scholar 

  • Tabatabai M.A., Bremner J.M.: Use ofp-nitrophenyl phosphate for assay of soil phosphatase activity.Soil Biol. Biochem. 1, 301–307 (1969).

    Article  CAS  Google Scholar 

  • Tabatabai M.A., Dick W.A.: Distribution and stability of pyrophosphatase in soils.Soil. Biol. Biochem. 6, 655–659 (1974).

    Google Scholar 

  • Trasar-Cepeda M.C., Sotres G.F., Ojea G.F.: Determinación de la actividad fosfatase en suelos Gallegos. Precisiones al metodo de Sarathchandra y Perrott.An. Edaf. Agrobiol. 44, 987–991 (1985).

    CAS  Google Scholar 

  • Zvyagintsev D.G., Aseyeva I.V., Babyeva I.P., Mirchink T.G.:Methods of Soil Microbiology and Biochemistry. (In Russian). Publ. House of Moscow State University, Moscow 1980.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Grunda, B., Rejšek, K. Method for estimation of acid phosphatase in woodland soils. Folia Microbiol 35, 443–453 (1990). https://doi.org/10.1007/BF02821414

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02821414

Keywords

Navigation