Abstract
A new modification was proposed for the estimation of acid phosphatase in soils, using the assay of released 4-nitrophenol. The procedure is simple and it is adapted to ecological conditions in the temperate zone. The technique is applicable to work with soils containing humus at different pH values, different amounts of clay-size particles and featuring different biological activities. The method was tested using six samples of steppe and woodland soils distinguished by different properties and was found to be suitable for the purpose.
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Brams W.H., McLaren A.D.: Phosphatase reactions in columns of soil.Soil Biol. Biochem. 6, 183–189 (1974).
Cervelli S., Nannipieri P., Ceccanti B., Sequi P.: Michaelis constant of soil acid phosphatase.Soil Biol. Biochem. 5, 841–845 (1973).
Dick W.S., Tabatabai M.A.: Hydrolysis of organic and inorganic phosphorus compounds added to soils.Geogerma 21, 175–182 (1978).
Drobníková V.: Factors influencing the determination of phosphatases in soil.Folia Microbiol. 6, 260–267 (1961).
Gerritse R.G., van Dijk H.: Determination of phosphatase activities of soils and animal wastes.Soil Biol. Biochem. 10, 545–551 (1978).
Harrison A.F.: Variation of four phosphatase properties in woodland soils.Soil Biol. Biochem. 11, 393–403 (1979).
Harrison A.F.: Relationship between intensity of phosphatase activity and physicochemical properties in woodland soils.Soil Biol. Biochem. 15, 93–99 (1983).
Harrison A.F., Pearce T.: Seasonal variation of phosphatase activity in woodland soils.Soil Biol. Biochem. 11, 405–410 (1979).
Ho I.: Acid phosphatase activity in forest soils.Forest Sci. 25, 567–568 (1979).
Hoffman G.: Eine photometrische Methode zur Bestimmung der Phosphatase-Aktivität im Boden.Zeitschr. Pfl. Bodenk. 118, 161–172 (1968).
Jirkovský R., Tržil J.:Chemical Tables for Laboratory Use. (In Czech) Práce, Prague 1973.
Khaziev F.C.:Systematic-ecological Analysis of the Fermentative Activity in Soils. (In Russian), Nauka, Moscow 1982.
Kramer N., Erdeli G.: Use of the method of phosphatase activity estimation in agrochemical conditions. (In Russian)Pochvovedenie 9, 99–102 (1959).
Malcolm R.E.: Assessment of phosphatase activity in soils.Soil Biol. Biochem. 15, 403–408 (1983).
Nikolsky B.P. (Ed.):The Chemist's Manual III. (In Russian) Khimiya, Moscow-Leningrad 1964.
Pang P.C.K., Kolenko H.: Phosphomonoesterase activity in forest soils.Soil Biol Biochem. 18, 35–39 (1986).
Ramírez-Martínez J.A.: Organic phosphorus mineralization and phosphatase activity in soils.Folia Microbiol. 13, 161–174 (1968).
Skujinš J.J., Braal L., McLaren A.D.: Characterization of phosphatase in terrestrial soil sterilized with an electron beam.Enzymologia 25, 125–133 (1962).
Sommer L.:Analytical Characteristics of Inorganic Ions and Substances (textbook). (In Czech) University J.E. Purkyně, Brno 1975.
Tabatabai M.A., Bremner J.M.: Use ofp-nitrophenyl phosphate for assay of soil phosphatase activity.Soil Biol. Biochem. 1, 301–307 (1969).
Tabatabai M.A., Dick W.A.: Distribution and stability of pyrophosphatase in soils.Soil. Biol. Biochem. 6, 655–659 (1974).
Trasar-Cepeda M.C., Sotres G.F., Ojea G.F.: Determinación de la actividad fosfatase en suelos Gallegos. Precisiones al metodo de Sarathchandra y Perrott.An. Edaf. Agrobiol. 44, 987–991 (1985).
Zvyagintsev D.G., Aseyeva I.V., Babyeva I.P., Mirchink T.G.:Methods of Soil Microbiology and Biochemistry. (In Russian). Publ. House of Moscow State University, Moscow 1980.
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Grunda, B., Rejšek, K. Method for estimation of acid phosphatase in woodland soils. Folia Microbiol 35, 443–453 (1990). https://doi.org/10.1007/BF02821414
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DOI: https://doi.org/10.1007/BF02821414