Skip to main content
Log in

Proline alleviates heavy metal stress inScenedesmus armatus

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Growth and some metabolic activities ofScenedesmus armatus grown in the presence of different heavy metals (Cd, Mn and Ni) with and without exogenously added proline (Pro) were monitored. The growth ofS. armatus cells (cell concentration, pigment and dry mass) was inhibited by all these heavy metals. Addition of Pro to the culture medium minimized the toxic effect of the metals. The growth rate was somewhat higher in Pro-containing cultures and started to decline I d later than in cultures containing heavy metals alone.S. armatus cells accumulated the added Pro in response to heavy metals. The accumulation correlated with protein content. Cd was the strongest inducer of Pro accumulation, Mn being the weakest. Cells accumulated nickel more than cadmium and manganese. Heavy metal-treated cells had increased peroxidase and catalase activities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adam M.S., Issa A.A.: Effect of manganese and calcium deficiency on the growth and oxygen exchange ofScenedesmus intermedius cultured for successive generations.Folia Microbiol.45, 353–358 (2000).

    CAS  Google Scholar 

  • Aebi M.: Catalasein vitro.Meth. Enzymol.105, 121–126 (1984).

    PubMed  CAS  Google Scholar 

  • Alia, Saradhi P.: Proline accumulation under metal stress.J. Plant Physiol.132, 554–558 (1992).

    Google Scholar 

  • Bates L.S., Walden R.P., Teare I.D.: Rapid determination of free proline for water stress studies.Plant & Soil39, 205–207 (1975).

    Article  Google Scholar 

  • Bradford M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding.Anal. Biochem.72, 248–254 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Costa G., Morel J.-L.: Water relations, gas exchange and amino acid content in Cd-treated lettuce.Plant Physiol. Biochem.32, 561–570 (1994).

    CAS  Google Scholar 

  • De Fillipis H., Ziegler H.: Effect of sublethal concentrations of zinc, cadmium and mercury on the photosynthetic carbon reduction cycle ofEuglena.J. Plant Physiol.142, 167–172 (1993).

    Google Scholar 

  • El-Enany A.E.: Shoot organogenesis and protein synthesis in tomato tissue cultures.Biol. Plant.39, 393–408 (1997).

    Google Scholar 

  • El-Enany A.E., Issa A.A.: Cyanobacteria as a biosorbent of heavy metals in sewage water.Environ. Toxicol. Pharmacol.8, 95–101 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Elstner E.F., Wagner G.A., Schutz W.: Activated oxygen in green plants in relation to stress situation.Curr. Top. Plant Physiol. Biochem.7, 159–187 (1988).

    Google Scholar 

  • Farago M.E.: Metal tolerant plants.Coord. Chem. Rev.36, 155–182 (1981).

    Article  CAS  Google Scholar 

  • Farago M.E., Mullen W.A.: Plants which accumulate metals—IV. A possible copper-proline complex from roots ofArmeria maritima.Inorg. Chem. Acta32, 193–194 (1979).

    Article  Google Scholar 

  • Grill E., Winnacker E-L., Zenk M.H.: Phytochelatins, a class of heavy metal-binding peptides from plants, are functionally analogous to metallothioneins.Proc. Nat. Acad. Sci. USA84, 439–443 (1987).

    Article  PubMed  CAS  Google Scholar 

  • Halliwell B., Gutteridge J.M.C.:Free Radicals in Biology and Medicine, pp. 188–266. Clarendon Press, Oxford 1988.

    Google Scholar 

  • Hammerschmidt R., Nuckles E.M., Kue J.: Association of enhanced peroxidase activity with induced systemic resistance of cucumber toColletotricum lagenarium.Physiol. Plant20, 73–82 (1982).

    CAS  Google Scholar 

  • Issa A.A., Adam M.S., Abdel-Basset R.: Effect of Ca2+ on the detoxification of Cd2+ byScenedesmus obliquus cells at low or high temperature.Folia Microbiol.43, 645–648 (1998).

    CAS  Google Scholar 

  • Issa A.A., Adam M.S.: Influence of selenium on toxicity of some heavy metals in the green algaScenedesmus obliquus.Folia Microbiol.44, 406–410 (1999).

    CAS  Google Scholar 

  • Lagriffoul A., Mocquot B., Mench M., Vangronsveld J.: Cadmium toxicity effect on growth, mineral and chlorophyll contents, and activities of stress related enzymes in young maize plants (Zea mays L.).Plant & Soil200, 241–250 (1998).

    Article  CAS  Google Scholar 

  • Lee K.C., Cunningham B.A., Paulsen G.M., Liang G.H., Moore R.B.: Lead effects on several enzymes and nitrogenous compounds in soybean leaf.J. Environ. Qual.5, 357–359 (1976).

    Article  CAS  Google Scholar 

  • Marker A.F.H., Crowther C.A., Gunn R.J.M.: Methanol and acetates solvents for estimating chlorophylla and phaeopigments by spectrophotometry.Arch. Hydrobiol. Beihft. Ergebniss. Limnol.14, 52–69 (1980).

    CAS  Google Scholar 

  • Metzner H., Rau H., Senger H.: Untersuchungen von Synchronisierbarkeit einzelner Pigmentmangel-Mutant vonChlorella.Planta65, 186–194 (1965).

    Article  CAS  Google Scholar 

  • Mocquot B., Vangronsveld J., Clijsters H., Mench M.: Copper toxicity in young maize (Zea mays L.) plants: effects on growth, mineral and chlorophyll contents, and enzyme activities.Plant & Soil182, 287–300 (1996).

    CAS  Google Scholar 

  • Nikolopoulos D., Manetas Y.: Compatible solutes andin vitro stability ofSalsola soda enzymes: proline incompatibility.Phytochemistry30, 411–413 (1991).

    Article  CAS  Google Scholar 

  • Olmos E., Piqueras J.R., Martinez-Solano E., Hellin E.: The subcellular localization of peroxidase and the implication of oxidative stress in hyperhydrated leaves of regenerated carnation plants.Plant Sci.130, 97–105 (1997).

    Article  CAS  Google Scholar 

  • Pandey R., Ganapathy P.S.: The proline enigma: NaCl-treatment and NaCl-sensitive callus lines ofCicer arietinum.Plant Sci.40, 13–17 (1985).

    Article  CAS  Google Scholar 

  • Sharma S.D., Chopra R.N.: Effect of lead nitrate and lead acetate on growth of the mossSemibarbula orientalis (Web.) at mary-growthin vitro.J. Plant Physiol.129, 242–249 (1987).

    Google Scholar 

  • Sigler K., Chaloupka J., Brozmanová J., Stadler N., Höfer M.: Oxidative stress in microorganisms—I. Microbialvs. higher cells—damage and defenses in relation to cell aging and death.Folia Microbiol.44, 587–624 (1999).

    Article  CAS  Google Scholar 

  • Steffens J.C.: The heavy metal-binding peptide of plants.Ann. Rev. Plant Physiol. Plant Mol. Biol.41, 553–575 (1994).

    Google Scholar 

  • Stein J.R.: Growth and mating ofGonium pectorale (Volvocales) in defined media.J. Phycol.2, 23–28 (1966).

    Article  Google Scholar 

  • Stroiniski A., Zielezinska M.: Cadmium effect on hydrogen peroxide, glutathione and phytochelatins levels in potato tuber.Acta Physiol. Plant.19, 127–135 (1997).

    Article  Google Scholar 

  • Tomsett A.B., Salt D.E., Mirana J., Thurman D.A.: Metallothioneins and metal tolerance.Asp. Appl. Biol.22, 365–372 (1989).

    Google Scholar 

  • Van Assche F., Cardinales C., Clijsters H.: Induction of enzymes capacity in plants as a result of heavy metal toxicity: dose-response relation inPhaseolus vulgaris L., treated with zinc and cadmium.Environ. Pollut.52, 103–115 (1988).

    Article  PubMed  Google Scholar 

  • Vymazal J.: Uptake of heavy metals byCladophora glomerata.Acta Hydrochem. Hydrobiol.18, 657–660 (1990).

    Article  CAS  Google Scholar 

  • Xiang C.B., Oliver D.J.: Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid inArabidopsis.Plant Cell10, 1539–1550 (1998).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

El-Enany, A.E., Issa, A.A. Proline alleviates heavy metal stress inScenedesmus armatus . Folia Microbiol 46, 227–230 (2001). https://doi.org/10.1007/BF02818538

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02818538

Keywords

Navigation