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Differentiation and apoptosis of human germinal center B-lymphocytes

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Abstract

An in vitro experimental model was developed to characterize the cellular and molecular factors that regulate germinal center (GC)B-cell differentiation and apoptosis. In the culture system that sustains the GC-B-cell survival, CD40L stimulation is essential for GC-B-cell proliferation and differentiation in the presence of 1L-2, IL-4, and IL-10. IL-2 and IL-4 promote proliferation of GC-B-cells, whereas IL-10 is required for generation of plasma cells. Generation of memory B cells requires CD40L, IL-2, IL-4, but not IL-10. There are two mechanisms that cause apoptosis. In the early stage, spontaneous apoptosis occurs in the absence of CD40 stimulation. Following CD40L stimulation, Fas-mediated apoptosis operates to eliminate GC-B-cells, unless activated GC-B-cells encounter a second signal via B-cell Ig receptors. Physiological significance of these findings is discussed.

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  1. Alton Ochsner Medical Foundation, Laboratory of Cellular Immunology, 1516 Jefferson Highway, 70121, New Orleans, LA

    Yong Sung Choi

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Choi, Y.S. Differentiation and apoptosis of human germinal center B-lymphocytes. Immunol Res 16, 161–174 (1997). https://doi.org/10.1007/BF02786360

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