Abstract
A flow-injection analysis (FIA) system coupled with an evanescent wave (EW) Biosensor employing total internal reflection of fluorescence radiation (TIRF) for the detection of polyaromatic hydrocarbon that intercalates into DNA is reported. A highly fluorescent intercalator, “ethidium bromide,” has been used as the reference compound for detection. The EW Biosensor was developed according to the procedure described earlier (1,2). Data on the analysis of Naphthalene, 3-methy cholanthrene, 7,12-dimethylbenz(a)anthracene, 1,2-benzanthracene, and some standard reference materials supplied by the National Institute of Standards and Technology are reported. The relative ability of the polyaromatic hydrocarbon to displace ethidium bromide, based on the relative binding ratio, is found to be on the order of 7,12-dimethylbenz[a]anthracene > 3-methylcholanthrene > 1,2-benzanthracene > napthalene.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Pandey, P. C. and Weetall, H. H. (1994),Anal. Chem. 66, 1236–1241.
Pandey, P. C. and Weetall, H. H. (1995),Anal. Chem. 67, 787–792.
Iyer, R. S., Coles, B. F., Raney, K. D., Thier, R., Guengerich, F. P., and Harris, T. M. (1994),J. Am. Chem. Soc. 116, 1603–1609.
Ye-XM, Kimura, K., and Patel, D. J. (1993),J. Am. Chem. Soc. 115, 9325.
Bailly, C. and Waring, M. J. (1993),Biochemistry 32, 5985–5993.
Meyaralmes, F. J. and Porschke, D. (1993),Biochemistry 32, 4246–4253.
Vanhoute, L. P. A., Vangarderen, C. J., and Patel, D. J. (1993),Biochemistry 32, 1667–1674.
Culp, S. J. and Beland, F. A. (1994),Carcinogenesis 15, 349–352.
Strothkamp, K. G. and Strothkamp, R. E. (1994),J. Chem. Education 71, 77–79.
Liu, F., Meadows, K. A., and Mcmillin, D. R. (1993),J. Am. Chem. Soc. 115, 6699–6704.
Pindur, U., Haber, M., and Satller, K. (1993),J. Chem. Education 70, 263–272.
Tang, P., Juang, C. L., and Harbison, G. S. (1990),Science 249, 70–72.
Glazer, A. N. and Rye, H. S. (1992),Nature 359, 859–861.
Nabiev, I., Chourpa, I., and Manfait, M. (1994),J. Phys. Chem. 98, 1344–1350.
Rye, H. S., Yua, S., Quesada, M. A., Haugland, R. P., Mathies, R. A., and Glazer, A. N. (1993),Method Enzymol. 217, 414–431.
Rye, H. S., Dabora, J. M., Quesada, M. A., Mathies, R. A., and Glazer, A. N. (1993),Anal. Biochem. 208, 144–150.
Hirschfeld, T. B. (1992), Canada patent 1,299,074.
Hirschfeld, T. B. (1990), Great Britain patent 2,190,189.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pandey, P.C., Weetall, H.H. Evanescent fluorobiosensor for the detection of polyaromatic hydrocarbon based on DNA intercalation. Appl Biochem Biotechnol 55, 87–94 (1995). https://doi.org/10.1007/BF02783551
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02783551