Abstract
Approximately 52% of the nuclear genome of great millet(Sorghum vulgare) consists of repetitive DNA which can be grouped into very fast, fast and slow components. The reiteration frequencies of the fast and slow reassociating components are {dy7000} and 92 respectively. Approximately 90% of the genome consists of repeated sequences interspersed amongst themselves and with single copy sequences. The interspersed repeat sequences are of three sizesviz. > 1·5 kilobase pairs, 0·5–1·0 kilobase pairs and 0·15–0·30 kilobase pairs while the size of the single copy sequences is 3·0 kilobase pairs. Hence the genome organization of great millet is essentially of a mixed type
Similar content being viewed by others
Abbreviations
- pg:
-
Picograms
- PIPES:
-
piperazine-N-N′-bis (2-ethanol sulphonic acid)
- Tris:
-
Tris (hydroxy methyl amino methane)
- kbp:
-
Kilobase pairs
- Tm :
-
melting temperature
- np:
-
nucleotide pairs
- HA:
-
hydroxyapatite
- RNase:
-
ribonuclease
References
Bendich, A. J. (1979) inMolecular Biology of Plants, (ed. L.Rubenstein) (New York: Academic Press) p. 1.
Bendich, A. J. and Anderson, R. S. (1977)Biochemistry,16, 4655.
Bonner, T. I., Brenner, D. J., Neufeld, B. R. and Britten, R. J. (1973)J. Mol. Biol.,81,123.
Braun, B. A., Schanke, K. E. and Graham, D. E. (1978)Nucleic Acids Res.,5,4283.
Britten, R. J., Graham, D. E. and Neufeld, B. R. (1974)Methods Enzymol.,29E, 363.
Flavell, R. B. and Smith, D. B. (1976)Heredity,37,231.
Graham, D. E., Neufeld, B. R., Davidson, E. M. and Britten, R. J. (1974)Cell,1,127.
Gupta, V. S., Gadre, S. R. and Ranjekar, P. K. (1981)Biochim. Biophys. Acta,656,147.
Gupta, V. S. and Ranjekar, P. K. (1981)J. Biosci.,3,417.
Gupta, V. S. and Ranjekar, P. K. (1982)Indian J. Biochem. Biophys.,19, 167.
Hake, S. and Walbot, V. (1980)Chromosoma,79, 251.
Joshi, C. P. (1982)Cytological and Molecular Biological Studies in Plant Genomes, Ph. D. Thesis, University of Poona, Poona.
Mahler, H. R. and Dutton, G. (1964)J. Mol. Biol.,10,157.
Preisler, R. S. and Thompson, W. F. (1981)J. Mol. Evol.,17,78.
Ranjekar, P. K., Pallota, D. and Lafontaine, J. G. (1976)Biochim. Biophys. Acta,425,30.
Seshadri, M. and Ranjekar, P. K. (1980)Biochim. Biophys. Acta,610, 211.
Smith, D. B. and Flavell, R. B. (1977)Biochim. Biophys. Acta,474, 82.
Ullman, J. S. and McCarthy, B. J. (1973)Biochim. Biophys. Acta,294, 405.
Wimpee, C. F. and Rawson, J. R. Y. (1979)Biochim. Biophys. Acta. 562, 192.
Zimmerman, J. L. and Goldberg, R. B. (1977)Chromosoma,59, 227.
Author information
Authors and Affiliations
Additional information
NCL communication No. 3527.
Rights and permissions
About this article
Cite this article
Sivaraman, L., Gupta, V.S. & Ranjekar, P.K. Molecular organization of great millet (Sorghum vulgare) DNA. J Biosci 6, 795–809 (1984). https://doi.org/10.1007/BF02716839
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02716839