Abstract
The dynamics of surface pressure(π) and of surface concentration (Г) of a14C radiolabeled β-lactoglobulin, a globular protein, adsorbed onto a spread monolayer of 1-monopalmitoyl-rac glycerol (named commonly as a monoglyceride), at the air-water interface were measured. The adsorption of14C labeled β lactoglobulin was enhanced at short times when C-16 monoglyceride of 73.4 and 24.7 μg was spread on the aqueous surface. However, the amounts of protein adsorbed at the steady state (after 10 h) were lowered with the values being 0.8 and 1.2 mg/m2, respectively. Spreading of such amount of monoglyceride that forms a dense packed monolayer onto air-water interface led to complete displacement of the protein from the aqueous surface after 2.5 h, possibly because of the surface pressure and exclusion effects.
Similar content being viewed by others
References
Bos, M. A. and Nylander, T., “Interaction between β-Lactoglobulin and Phospholipids at the Air-Water Interface,”Langmuir,12, 2791 (1996).
Boury, F., Ivanova, I., Panaiotov, I. and Proust, J. E., “Dilatational Properties of poly(D, L-lactic acid) and Bovine Serum Albumin Monolayers Spread at the Air/Water Interface,”Langmuir,11, 599 (1995).
Boury, F., Ivanova, I., Panaiotov, I. and Proust, J. E., “Dilatational Properties of poly(D, L-lactic acid) and Bovine Serum Albumin Monolayers Formed from Spreading an Oil-in-Water Emulsion at the Air-Water Interface,”Langmuir,11, 2131 (1995).
Brewkink, E., Demel, R. A., De Korte-Kool, G. and De Kruijff, B., “SecA Insertion into Phospholipids is Stimulated by Negatively Charged Lipids by ATP: A Monolayer Study,”Biochemistry,31, 1119 (1992).
Cho, D., Narsimhan, G. and Franses, E. I., “Interactions of Spread Lecithin Monolayers with Bovine Serum Albumin in Aqueous Solution,”Langmuir,13, 4710 (1997).
Clark, D. C., Mackie, A. R., Wilde, P. J. and Wilson, D. R., “Differences in the Structure and Dynamics of the Adsorbed Layers in Protein-Stabilized Model Foams and Emulsions,”Faraday Discuss,98, 253 (1994).
Clark, D. C., Wilde, P. J. and Wilson, D. R., “Competitive Adsorption in Oil-in-Water Emulsions Containing α-Lactalbumin and β-Lactoglobulin,”Colloids and Surfaces,59, 209 (1991).
Coke, M., Wilde, P. J., Russel, E. J. and Clark, D. C., “The Influence of Surface Composition and Molecular Diffusion on the Stability of Foams Formed from Protein/Surfactant Mixtures,”J. Colloid Interface Sci.,138, 489 (1990).
Cornec, M., Mackie, A. R., Wilde, P. J. and Clark, D. C., “Competitive Adsorption of β-Lactoglobulin and β-Casein with Span 80 at the Oil-Water Interface and the Effects on Emulsion Behaviour,”Colloids Surfaces A: Physicochem. Eng. Aspects,114, 237 (1996).
Cornell, D. G. and Carroll, R. J., “Miscibility in Lipid-Protein Monolayers,”J. Colloid Interface Sci.,108, 226 (1985).
Cornell, D. G. and Patterson, D. L., “Interaction of Phospholipids in Monolayers with β-Lactoglobulin Adsorbed from Solution,”J. Agric. Food Chem.,37, 1455 (1989).
Cornell, D. G., “Lipid-Protein Interactions in Monolayers: Egg Yolk Phosphatidic Acid and β-Lactoglobulin”J. Colloid Interface Sci.,88, 536 (1982).
Cornell, D. G., Patterson, D. L. and Hoban, N., “The Interaction of Phospholipids in Monolayers with Bovine Serum Albumin and oc-Lactalbumin Adsorbed from Solution,”J. Colloid Interface Sci.,140, 428 (1990).
Courthaudon, J. L., Dickinson, E., Matsumara, Y. and Clark, D.C., “Competitive Adsorption of β-Lactoglobulin+Tween 20 at the Oil-Water Interface,”Colloids Surfaces,56, 293 (1991).
Du, Y., An, J., Tang, J. and Jiang, L., “A Study of the Interaction between Glycolipids of Different Hydrophobicities and Glucose Oxidase by a Monolayer Technique,”Colloids and Surfaces B,7, 129 (1996).
Ewers, W. E. and Sutherland, K. L., “The Role of Surface Transport in the Stability and Breakdown of Foams,”Aust. J. Sci. Res. Ser. A,5, 697 (1952).
Gehlert, U., Weidemann, G. and Vollhardt, D., “Morphological Features in 1-Monoglyceride Monolayers,”J. Colloid Interface Sci.,174, 392 (1995).
Grimard, R., Tancrede, P. and Gicquaud, C., “Interaction of Actin with Positively Charched Phospholipids: a Monolayer Study,”Biochem. Biophys. Res. Com.,190, 1017 (1993).
Hunter, J. R., Kilpatrick, P. K. and Carbonell, R. G., “β-Casein Adsorption at the Air/Water Interface,”J. Colloid Interface Sci.,142, 429 (1991).
Ibdah, J. A. and Phillips, M. C., “Effect of Lipid Composition and Packing on the Adsorption of Apolipoprotein A-I to Lipid Monolayers,”Biochemistry,27, 7155 (1988).
Israelachvili, J., “The Science and Applications of Emulsions-an Overview,”Colloids and Surfaces A,91, 1 (1994).
Kozorac, Z., Dhathathregan, A. and Möbius, D., “Adsorption of Cytochrome c to Phospholipid Monolayers Studied by Reflection Spectroscopy,”FEBS,229, 372 (1988).
MacRitchie, F., “Spread Monolayers of Proteins,”Adv. Protein Chem.,32, 283 (1978).
McClements, D. J., “Food Emulsions: Principles, Practice and Techniques,” CRC Press (1998).
Narsimhan, G. and Uraizee, F., “Kinetics of Adsorption of Globular Proteins at an Air-Water Interface,”Biotechnol. Prog.,8, 187 (1992).
Nylander, T. in “Proteins at Liquid Interfaces,” Möbius, D. and Miller, R. Eds., Elsevier (1998).
Quinn, P. J. and Dawson, R. M. C., “The Interaction of Cytochrome c with Monolayers of Phosphatidylethanolamine,”Biochem J.,113, 791 (1969).
Rahman, A. and Sherman, P., “Interaction of Milk Proteins with Monoglycerides and Diglycerides,”Colloid & Polymer Sci.,260, 1035 (1982).
Rodriguez Nino, M.R. and Rodriguez Patino, J.M., “Surface Tension of Bovine Serum Albumin and Tween 20 at the Air-Water Interface,”JAOCS,75, 1233 (1998).
Rodriguez Nino, M.R., Wilde, P. J., Clark, D. C. and Rodriguez Patino, J.M., “Surface Dilational Properties of Protein and Lipid Films at the Air-Water Interface,”Langmuir,14, 2160 (1998).
Sarker, D. K., Wilde, P. J. and Clark, D. C., “Competitive Adsorption of L-oc-Lysophosphatidylcholine/β-Lactoglobulin Mixtures at Interfaces of Foams and Foam Lamellae,”Colloids Surfaces B: Biointerfaces,3, 349 (1995).
Wilde, P. J. and Clark, D. C., “The Competitive Displacement of β-Lactoglobulin by Tween 20 from Oil-Water and Air-Water Interfaces,”J. Colloid Interface Sci.,155, 48 (1993).
Xu, S. and Damodaran, S., “Calibration of Radiotracer Method to Study Protein Adsorption at Interfaces,”J. Colloid Interface Sci.,157, 485 (1993).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cho, D., Cornec, M. Adsorptive behavior of a globular protein with a monoglyceride monolayer spread on the aqueous surface. Korean J. Chem. Eng. 16, 371–376 (1999). https://doi.org/10.1007/BF02707127
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02707127