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Influence of inhibitory compounds and minor sugars on xylitol production by Debaryomyces hansenii

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Abstract

To obtain in-depth information on the overall metabolic behavior of the new good xylitol producer Debaryomyces hansenii UFV-170, batch bioconversions were carried out using semisynthetic media with compositions simulating those of typical acidic hemicellulose hydrolysates of sugarcane bagasse. For this purpose, we used media containing glucose (4.3–6.5 g/L), xylose (60.1–92.1 g/L), or arabinose (5.9–9.2 g/L), or binary or ternary mixtures of them in either the presence or absence of typical inhibitors of acidic hydrolysates, such as furfural (1.0–5.0 g/L), hydroxymethylfurfural (0.01–0.30 g/L), acetic acid (0.5–3.0 g/L), and vanillin (0.5–3.0 g/L). D. hansenii exhibited a good tolerance to high sugar concentrations as well as to the presence of inhibiting compounds in the fermentation media. It was able to produce xylitol only from xylose, arabitol from arabinose, and no glucitol from glucose. Arabinose metabolization was incomplete, while ethanol was mainly produced from glucose and, to a lesser less extent, from xylose and arabinose. The results suggest potential application of this strain in xyloseto-xylitol bioconversion from complex xylose media from lignocellulosic materials.

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Authors and Affiliations

  1. Department of Microbiology, Instituto de Biotecnologia Aplicada à Agropecuária, Federal University of Vicosa, Av. P. H. Rolfs s/n, 36570-000, Viçosa, Minas Gerais, Brazil

    Fábio C. Sampaio, Flávia M. Lopes Passos & Célia Alencar de Moraes

  2. Department of Chemical and Process Engineering, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy

    Paolo Torre, Patrizia Perego & Attilio Converti

Authors
  1. Fábio C. Sampaio
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  2. Paolo Torre
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  3. Flávia M. Lopes Passos
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  4. Célia Alencar de Moraes
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  5. Patrizia Perego
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  6. Attilio Converti
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Correspondence to Attilio Converti.

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Sampaio, F.C., Torre, P., Passos, F.M.L. et al. Influence of inhibitory compounds and minor sugars on xylitol production by Debaryomyces hansenii . Appl Biochem Biotechnol 136, 165–181 (2007). https://doi.org/10.1007/BF02686021

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  • DOI: https://doi.org/10.1007/BF02686021

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