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Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.)

  • Plant Cellular and Developmental Biology
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Summary

Multiple shoots were produced, from cotyledonary node explants of pea (Pisum sativum L.) cultured on MS medium containing 1 mg 1−1 BAP. The number of buds formed could be increased by scraping the nodes before culture or by increasing the cytokinin concentation. However, cytokinin levels over 5 mg 1−1 increasingly produced shoots that were vitrified and dificult to root. With all the genotypes tested, developing buds were visibile as soon as 5 days after culture and elongated shoots could be removed after 21 days., Histological studies indicated, that the buds and shoots were formed from superficial layers of tissue. The efficiency of this regeneration system compared favorably with previosly published methods. The rapid, genotype-independent, high frequency system described here may be of use in the production of transgenic pea plants.

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Authors and Affiliations

  1. Plant Biotechnology Institute, National Research Council of Canada, S7N OW9, Saskatoon, Saskatchewan, Canada

    Jennifer A. Jackson & Shaun L. A. Hobbs

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  1. Jennifer A. Jackson
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  2. Shaun L. A. Hobbs
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Jackson, J.A., Hobbs, S.L.A. Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.). In Vitro Cell Dev Biol 26, 835–838 (1990). https://doi.org/10.1007/BF02623626

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  • DOI: https://doi.org/10.1007/BF02623626

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