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Laminin stimuliert die schnelle Restitution der humanen Kolonschleimhaut nach Gallensäureschädigung in vitro

Laminin stimulates rapid restitution following bile acid injury of the human colonic mucosa in vitro

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Zusammenfassung

In dieser Studie wurde der Einfluß von verschiedenen Faktoren auf die schnelle Restitution der humanen Kolonschleimhaut in vitro nach Schädigung mit Natriumdesoxycholat untersucht. Mit einer computergesteuerten Echtzeitmorphometrie wurde der zeitliche Ablauf der Restitution gemessen. Unmittelbar nach Schädigung waren 51% der Schleimhautoberfläche geschädigt, nach 5 h Restitution nur 26%. Kontinuierliche luminale Natriumde-soxycholatexposition während der Restitution oder kalziumfreie Nährlösung inhibierten die Restitution vollständig. Luminale Gabe von Antifibronektin-Antiserum oder von humanem Fibronektin zeigten keinen Einfluß auf die Restitution. Ein monoklonaler, gegen Laminin gerichteter Antikörper führte zu einer Restschädigung von 42%. Luminale Gabe von humanem Laminin stimulierte die schnelle Restitution, so daß nach 5 h nur mehr 8% der Schleimhautoberfläche geschädigt waren. Die transmukosale Potentialdifferenz und der Kurzschlußstrom wurden gemessen, der Widerstand berechnet. Nach luminaler Gallensäureexposition kam es zu einem Abfall der Potentialdifferenz, des Kurzschlußstroms und des Widerstandes. Die schnelle Restitution des humanen Kolons benötigt Kalzium, wird durch einen gegen Laminin gerichteten monoklonalen Antikörper gehemmt, durch Natriumdesoxycholat verhindert und weder durch Antifibronektin-Antiserum noch durch Fibronektin beeinflußt. Hingegen führt die luminale Gabe von Laminin zu einer Stimulation der schnellen Restitution.

Summary

This study investigated the influence of various factors on rapid epithelial restitution in the human colonic mucosa in vitro following superficial mucosal injury produced by luminal exposure to sodium deoxycholate. The morphologic time course of restitution was assessed with real-time morphometry. Immediately after injury 51% of the mucosal surface was damaged. Following undisturbed restitution 26% remained injured after 5 hours. Continuous luminal exposure to sodium deoxycholate during the restitution period or removal of calcium from the nutrient solution inhibited restitution completely. Luminal anti-fibronectin antiserum or purified fibronectin did not influence restitution. A monoclonal antibody against laminin caused a residual damage of 42% whereas luminal purified laminin accelerated rapid epithelial restitution significantly as only 8% of the mucosal surface were damaged after 5 hours. Transmucosal potential difference and short-circuit current were measured, resistance was calculated. Bile salt injury produced a drop of potential difference, short-circuit current and resistance. These results show that rapid epithelial restitution of the human colon in vitro requires calcium, is impaired by anti-laminin antibody, completely inhibited by sodium deoxycholate, but unaffected by anti-fibronectin antiserum and fibronectin. Luminal addition of laminin accelerates rapid epithelial restitution.

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Authors and Affiliations

  1. I. Chirurgische Universitätsklinik, Alser Straße 4, A-1090, Wien

    M. Riegler,  W. Feil, T. Sogukoglu, G. Hamilton, G. Bischof, E. Wenzl & R. Schiessel

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  1. M. Riegler
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  2. W. Feil
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  3. T. Sogukoglu
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  4. G. Hamilton
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  5. G. Bischof
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  6. E. Wenzl
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  7. R. Schiessel
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Univ.-Prof. Dr.A. Fritsch zum 65. Geburtstag gewidmet.

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Riegler, M., Feil, W., Sogukoglu, T. et al. Laminin stimuliert die schnelle Restitution der humanen Kolonschleimhaut nach Gallensäureschädigung in vitro. Acta Chir Austriaca 24, 364–371 (1992). https://doi.org/10.1007/BF02602076

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