Skip to main content
SpringerLink
Log in

Relationship between body fat mass, carbohydrate tolerance and iri response during glucose infusion in subjects with early diabetes

  • Published:
Acta diabetologia latina Aims and scope Submit manuscript

Summary

We have studied the interrelationship of total body fat mass, carbohydrate tolerance and IRI response in 17 non-obese and obese subjects, who were suspected of having early diabetes. We carried out an i.v. glucose infusion test consisting of a priming injection of 0.33 g/kg followed by constant glucose infusion of 12 mg/kg/min in all persons. Total body fat mass was estimated by the tritium dilution method. There was a positive correlation of body fat mass, fasting glucose concentration and blood glucose concentration at 150 min as well as a strong correlation between body fat mass and BG area 60–120 min as parameters of carbohydrate tolerance in all subjects, i.e. the degree of carbohydrate intolerance was directly related to the quantity of total body fat mass. A similar correlation was found when the non-obese and obese groups were analyzed separately. In neither group did total body fat mass correlate with parameters of IRI response. In obese subjects with pathological carbohydrate tolerance, however, a positive correlation of basal IRI concentration and total body fat mass was found. Furthermore, a close relation between basal IRI level and parameters of carbohydrate tolerance could be demonstrated in obese subjects. The present study failed to demonstrate any correlation of parameters of carbohydrate tolerance and glucose-induced IRI response in either group. Thus, the significant relationship between body fat mass and degree of carbohydrate intolerance indicates that total body fat mass plays an important role in the disturbance of blood glucose homeostasis in early diabetes with and without obesity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Archer J. A., Gordon P., Gavin J. R., Lesniak M., Roth J.: Insulin receptors in human circulating lymphocytes: application to the study of insulin resistance in man - J. clin. Endocr.36, 627, 1973.

    Article  PubMed  CAS  Google Scholar 

  2. Archer J. A., Gordon P., Roth J.: Defect in insulin binding to receptors in obese man. Amelioration with caloric restriction - J. clin. Invest.55, 166, 1975.

    PubMed  CAS  Google Scholar 

  3. Bagdade J. D., Bierman E. L., Porte D. Jr.: The significance of basal insulin levels in the evaluation of the insulin response to glucose in diabetic and nondiabetic subjects - J. clin. Invest.46, 1549, 1967.

    PubMed  CAS  Google Scholar 

  4. Beck-Nielsen H., Pedersen O., Bagger J. P., Sørensen N. S.: The insulin receptor in normal and obese persons - Acta endocr. (Kbh.)83, 565, 1976.

    CAS  Google Scholar 

  5. Björntorp P.: Disturbances in the regulation of food intake - Advanc. psychosom. Med. (Basel)7, 116, 1972.

    Google Scholar 

  6. Björntorp P., De Jounge K., Sjöstrom L., Sullivan L.: The effect of physical training on insulin production in obesity - Metabolism19, 631, 1970.

    Article  PubMed  Google Scholar 

  7. Butterfield W. J. H., Hanley T., Whichelow M. J.: Peripheral metabolism of glucose and free fatty acids during oral glucose tolerance tests - Metabolism14, 851, 1965.

    Article  PubMed  CAS  Google Scholar 

  8. Deckert T., Hegerup L.: Serum insulin in normal and obese persons- Acta med. scand.182, 225, 1967.

    Article  PubMed  CAS  Google Scholar 

  9. El-Khodary A. Z., Ball M. F., Oweiss I. M., Canary J. J.: Insulin secretion and body composition - Metabolism21, 641, 1972.

    Article  PubMed  CAS  Google Scholar 

  10. Faulhaber J. D., Petruzzi N., Eble H., Ditschuneit H.:In vitro — Untersuchungen über den Fettstoffwechsel isolierter menschlicher Fettzellen in Abhängigkeit von der Zellgrösse: die durch Adrenalin induzierte Lipolyse- Hormone metab. Res.1, 80, 1969.

    Article  CAS  Google Scholar 

  11. Fischer U.: Insulinskeretionsrate und Pankreasdurchblutung des Hundes - Promotion B, Greifswald 1974.

    Google Scholar 

  12. Ginsberg H., Kimmerling G., Olefsky J. M., Reaven G. M.: Demonstration of insulin resistance in untreated adult onset diabetic subjects with fasting hyperglycemia - J. clin. Invest.55, 454, 1975.

    PubMed  CAS  Google Scholar 

  13. Gries F. A., Berger M., Neumann M., Preiss H., Liebermeister H., Hesse-Wortmann C., Jahnke K.: Effects of norepinephrine, theophylline and dibutyryl cyclic AMP onin vitro lipolysis of human adipose tissue in obesity - Diabetologia8, 75, 972.

  14. Hepp K. D.: Studies on the mechanism of insulin action: basic concept and clinical implications - Diabetologia13, 177, 1977.

    Article  PubMed  CAS  Google Scholar 

  15. Hoffman W. S.: A rapid photoelectric method for determination of glucose in blood and urine - J. biol. Chem.120, 51, 1937.

    CAS  Google Scholar 

  16. Knittle J. L., Ginsberg-Fellner F.: Effect of weight reduction onin vitro adipose tissue lipolysis and cellularity in obese adolescents and adults - Diabetes21, 754, 1972.

    PubMed  CAS  Google Scholar 

  17. Lisch H., Sailer S., Sandhofer F., Braunsteiner H.: Untersuchungen an isolierten menschlichen Fettzellen verschiedener Fettgewebsregionen. II. Beziehungen zwischen Zellvolumen und basaler Lipolyse - Klin. Wschr.48, 1353, 1970.

    Article  PubMed  CAS  Google Scholar 

  18. Michaelis D., Schulz B., Neumann I., Wulfert P., Ziegler M.: Diagnostik der Vorstadien des Diabetes mellitus. 4. Mitteilung: Alteration der biphasischen Insulinsekretion und des Fettstoffwechsels in Abhängigkeit vom Grad der Kohlenhydratintoleranz bei normgewichtigen Personen - Dtsch. Gesundh.-Wes.31, 588, 1976.

    CAS  Google Scholar 

  19. Möhr M., Jonsen D.: Tabellen zur Beurteilung des Körpergewichts erwachsener Männer und Frauen nach ihrem Optimalgewicht - Z. ärztl. Fortbild.66, 1052, 1972.

    Google Scholar 

  20. Neumann I., Michaelis D., Schulz B., Michael R., Schneider H., Hahnefeld M.: Beeinflussung hormonell-metabolischer und morphologischer Parameter der Adipositas durch Gewichtsreduktion. Mitteilung: Verhalten des Kohlenhydratstoffwechsels und der Insulinsekretion- Dtsch. Gesundh.-Wes.29, 1172, 1974.

    CAS  Google Scholar 

  21. Olefsky J. M., Reaven G. M.: Decreased insulin binding of lymphocytes from diabetic subjects - J. clin. Invest.54, 1323, 1974.

    PubMed  CAS  Google Scholar 

  22. Olefsky J. M., Reaven G. M.: Insulin binding in diabetes. Relationship with plasma insulin levels and insulin sensitivity - Diabetes26, 680, 1977.

    PubMed  CAS  Google Scholar 

  23. Olefsky J. M., Reaven G. M., Farquhar J. W.: Effects of weight reduction on obesity. Studies of lipid and carbohydrate metabolism in normal and hyperlipoproteinemic subjects - J. clin. Invest.53, 64, 1974.

    Article  PubMed  CAS  Google Scholar 

  24. Rabinowitz D., Zierler K. L.: Forearm metabolism in obesity and its response to intra-arterial insulin. Characterization of insulin resistance and evidence for adaptive hyperinsulinism - J. clin. Invest.41, 2173, 1962.

    PubMed  CAS  Google Scholar 

  25. Ratzmann K. P., Knospe H.: Vergleichende Untersuchungen zur Bestimmung des Fettanteils am Körpergewicht mittels Caliprometrie und Tritiumdilutionsmethode- Dtsch. Gesundh.-Wes.32, 1129, 1977.

    Google Scholar 

  26. Ratzmann K. P., Männchen F., Meyer L. W., Baufeld W.: Interrelation zwischen Blutglukose, peripheren IRI-Konzentrationen und Lipidparametern bei Adipositas mit normaler Glukosetoleranz - Dtsch. Gesundh.-Wes.30, 538, 1974.

    Google Scholar 

  27. Ratzmann K. P., Michaelis D., Neumann I., Schulz B., Mohnike W., Hildmann W.: Diagnostik der Vorstadien des Diabetes mellitus. 5. Mitteilung: Aussagewert der IRI-Reaktionsmuster nach oraler Glukosebelastung - Dtsch. Gesundh.-Wes.31, 493, 1976.

    Google Scholar 

  28. Salans L. B., Knittle J. L., Hirsch J.: The role of adipose cell size and adipose tissue sensitivity in the carbohydrate intolerance of human obesity - J. clin. Invest.47, 153, 1968.

    PubMed  CAS  Google Scholar 

  29. Schulz B., Michaelis D., Neumann I., Gottschling H. D., Bibergeil H.: Diagnostik der Vorstadien des Diabetes. 5. Mitteilung: Kohlenhydrattoleranz und Insulinverhalten im GIT bei Übergewichtigen und Adipösen mit Protodiabetesverdacht- Dtsch. Gesundh.-Wes.31, 643, 1976.

    Google Scholar 

  30. Stephan F., Reville Ph., Thierry R., Schlenger J. L.: Correlation between plasma insulin and body weight in obesity, anorexia nervosa and diabetes mellitus - Diabetologia8, 196, 1972.

    Article  PubMed  CAS  Google Scholar 

  31. Stern J. S., Batchelor B. R., Hollander N., Cohn C. K., Hirsch J.: Adipose-cell size and immunoreactive insulin levels in obese and normal-weight adults - Lancet2, 948, 1972.

    Article  PubMed  CAS  Google Scholar 

  32. West T. E. T., Owen D., Sönsken P. H., Srivastava C. M., Tompkins C. V., Nabarro J. D. N.: Metabolic responses to monocomponent human insulin infusion in normal subjects and patients with liver and endocrine disease - Clin. Endocr.4, 573, 1975.

    PubMed  CAS  Google Scholar 

  33. WHO: Diabetes mellitus. Report of a WHO Expert Committee - Wld Hlth Org. techn. Rep. Ser. 310, 1965.

  34. Wüstenberg P. W., Holtz M., Krüger M., Neumann G., Potschwadek B., Rohmann H.: Gesamtkörperfettbestimmung mit der Tritiumdilutionsmethode und ihre Korrelation zur Kohlenhydrattoleranz - Z. inn. Med.29, 1004, 1974.

    Google Scholar 

  35. Ziegler M., Kang K., Gens J., Johannsen B., Michael R., Michaelis D.: Anwendung des Radioimmuntestbestecks ‘Insulin’ bei Insulinsekretionsstudien -VIII. Symp. d. Sekt. Nuklearmedizin in der Ges. Med. Radiologie, Reinhardsbrunn 1971.

  36. Zinder O., Arad R., Shapiro B.: Effect of cell size on the metabolism of isolated fat cells Israel J. med. Sci.3, 787, 1967.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zentralinstitut für Diabetes ‘Gerhardt Katsch’ Clinical Research Unit and Department of Experimental Diabetes Research, Karlsburg

    Klaus-Peter Ratzmann, Siegfried Knospe, Peter Heinke & Bernd Schulz

Authors
  1. Klaus-Peter Ratzmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Siegfried Knospe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter Heinke
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bernd Schulz
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Investigation performed within the medical research project ‘Diabetes mellitus and disturbances of lipid metabolism’, Ministry of Health, GDR.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ratzmann, KP., Knospe, S., Heinke, P. et al. Relationship between body fat mass, carbohydrate tolerance and iri response during glucose infusion in subjects with early diabetes. Acta diabet. lat 16, 67–75 (1979). https://doi.org/10.1007/BF02590765

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02590765

Key-words

Search

Navigation