Summary
In vitro precipitation of calcium phosphate was carried out using a one-dimensional double diffusion system in agar gel. Bovine dentin phosphophoryn enhanced the sharpness of the precipitation bands, although it reduced the total amount of the precipitates. Dephosphorylated phosphophoryn has no effect on the pattern of precipitates. Therefore, phosphophoryn is thought to raise the local density of nucleation in spite of its inhibitory activity on apatite formation.
Similar content being viewed by others
References
Veis A (1984) Bones and teeth. In: Piez KA, Reddi AH (eds) Extracellular matrix biochemistry. Elsevier, New York, pp 329–374
Nawrot CF, Campbell DJ, Schroeder JK, Van Valkenburg M (1976) Dental phosphoprotein-induced formation of hydroxylapatite during in vitro sysnthesis of amorphous calcium phosphate. Biochemistry 15:3445–3449
Termine JD, Conn KM (1976) Inhibition of apatite formation by phosphorylated metabolites and macromolecules. Calcif Tissue Res 22:149–157
Termine JD, Eanes ED, Conn KM (1980) Phosphoprotein modulation of apatite crystallization. Calcif Tissue Int 31:247–251
Pokrić B, Pučar Z (1979) Precipitation of calcium phosphate under conditions of double diffusion in collagen and gels of gelatin and agar. Calcif Tissue Int 27:171–176
Kuboki Y, Fujisawa R, Aoyama K, Sasaki S (1979) Calcium-specific precipitation of dentin phosphoprotein: a new method of purification and the significance for the mechanism of calcifications. J Dent Res 58:1926–1932
Fujisawa R, Takagi T, Kuboki Y, Sasaki S (1984) Systematic purification of free and matrix-bound phosphophoryns of bovine dentin: presence of matrix-bound phosphophoryn as a distinct molecular entity. Calcif Tissue Int 36:239–242
Fujisawa R, Kuboki Y, Sasaki S (in press) Changes in interaction of bovine dentin phosphophoryn with calcium and hydroxyapatite by chemical modifications. Calcif Tissue Int
Connerty HV, Briggs AR (1966) Determination of serum calcium by means of orthocreasolphthalein complexone. Am J Clin Pathol 45:290–296
Chen PS Jr, Toribara TY, Warner H (1956) Microdetermination of phosphorus. Anal Chem 28:1756–1758
Hayek E (1967) Die Mineralsubstanz der Knochen. Klinische Wochenschrift 45:857–863
Pučar Z, Pokrić B, Graovac A (1974) Precipitation in gels under conditions of double diffusion: critical concentrations of the precipitating components. Anal Chem 46:403–409
Weinstock M, Leblond CP (1973) Radioautographic visualization of the deposition of a phosphoprotein at the mineralization front in the dentin of the rat incisor. J Cell Biol 56:838–845
Carmichael DJ, Chovelon A, Pearson CH (1975) The composition of the insoluble collagenous matrix of bovine predentine. Calcif Tissue Res 17:263–271
Jontell M, Linde A (1983) Non-collagenous proteins of predentine from dentinogenically active bovine teeth. Biochem J 214:769–776
Takagi Y, Fujisawa R, Sasaki S (1986) Identification of dentin phosphophoryn localization by histochemical stainings. Connect Tissue Res 14:279–292
Nakamura O, Gohda E, Ozawa M, Senba I, Miyazaki H, Murakami T, Daikuhara Y (1985) Immunohistochemical studies with a monoclonal antibody on the distribution of phosphophoryn in predentin and dentin. Calcif Tissue Int 37:491–500
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fujisawa, R., Kuboki, Y. & Sasaki, S. Effects of dentin phosphophoryn on precipitation of calcium phosphate in gelin vitro . Calcif Tissue Int 41, 44–47 (1987). https://doi.org/10.1007/BF02555130
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02555130