Summary
Identification of cultivars and selections, pedigree analysis, estimation of genetic relatedness and genome mapping — all of these objects are achieved with various methods of DNA fingerprinting which can distinguish even closely related genotypes. Initial results were obtained by a variation of the well-known RFLP-technique (Restriction Fragment Length Polymorphism): hybridization of restriction enzyme-digested DNA samples with hypervariable minisatellite DNA probes. Technical improvements, including a number of additional DNA probes and various DNA fragment detection methods, have since been published. With the recent advent of the PCR technique (Polymerase Chain Reaction) and PCR-based methods like RAPD (Random Amplified Polymorphic DNA), DNA fingerprinting has widened its applicability and attracted a growing number of scientists.
Similar content being viewed by others
References
Abbott, A., L. Belthoff, S. Rajapakse, R. Ballard, W.V. Baird, R. Monet, R. Scorza, P Morgans & A. Callahan,1992. Development of a genetic linkage map of peach. Plant Genome I, San Diego Nov. 9–11, 1992:44.
Aruna, M., P. Ozias-Akins, M.E. Austin & G. Kochert, 1993. Genetic relatedness among rabbiteye blueberry (Vaccinium ashei) cultivars determined by DNA amplification using single primers of arbitrary sequence. Genome 36: 971–977.
Baird, E., S. Cooper-Bland, R. Waugh, M. DeMaine & W. Powell, 1992. Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA (RAPD) markers. Mol. Gen. Genet. 233:469–475.
Boury, S., I. Lutz, M.-C. Gavalda, F. Guidet & A. Schlesser, 1992. Empreintes génétiques du chou-fleur par RAPD et vérification de la pureté hybride Fl d'un lot de semences. Agronomie 12: 669–681.
Braithwaite, K.S. & J.M. Manners, 1989. Human hypervariable minisatellite probes detect DNA polymorphisms in the fungusColletotrichum gloeosporioides. Curr. Genet. 16: 473–475.
Brown, P.T.H., F.D. Lange, E. Kranz & H. Lörz, 1993. Analysis of single protoplasts and regenerated plants by PCR and RAPD technology. Mol. Gen. Genet. 237: 311–317.
Büscher, N., E. Zyprian & R. Blaich, 1993. Identification of grapevine cultivars by DNA analyses — Pitfalls of random amplified polymorphic DNA techniques using 10mer primers. Vitis 32: 187–188.
Cai, Q., C. Guy & G.A. Moore (1992) Genetic mapping of random amplified polymorphic DNA (RAPD) markers inCitrus. Plant Genome I, San Diego Nov. 9–11, 1992: 20.
Chaparro, J., D. Werner, D. O'Malley & R. Sederoff, 1992. Targetted mapping and linkage analysis in peach. Plant Genome 1, San Diego Nov 9–11, 1992: 21.
Collins, G.G. & R.H. Symons, 1993. Polymorphisms in grapevine DNA detected by the RAPD PCR technique. Plant Mol. Biol. Reporter 11: 105–112.
Dallas, J.F., 1988. Detection of DNA “fingerprints” of cultivated rice by hybridization with a human minisatellite probe. Proc. Nad. Acad. Sci. USA 85: 6831–6835.
Dirlewanger, E. & C. Bodo, 1994. Molecular genetic mapping of peach. Eucarpia Fruit Breeding Sect. Meeting, Aug. 30–Sept. 3, 1993. In: H. Schmidt and M. Kellerhals (Eds.) Progress in temperate fruit breeding, pp. 309–311. Kluwer Academic Publishers, Dordrecht.
Dunemann, F., 1994. Genetic relationships inMalus evaluated by RAPD “fingerprinting” of cultivars and wild species. In: H. Schmidt and M. Kellerhals (Eds.) Progress in temperate fruit breeding, pp. 293–300. Kluwer Academic Publishers, Dordrecht.
European Patent Office, Publication number 0 534 858 Al. (Applicant: Keygene N.V., Wageningen, The Netherlands).
Fauré, S., J.L. Noyer, J.P. Horry, F. Bakry, C. Lanaud & D. Gonzalez de Léon, 1992. Genetic mapping of the diploid banana genome using molecular markers. Plant Genome I San Diego Nov. 9–11, 1992: 46.
Gardiner, S.E., J.M. Zhu, H.C.M. Whitehead & C. Madie, 1994. The New Zealand apple genome mapping project. In: H. Schmidt and M. Kellerhals (Eds.) Progress in temperate fruit breeding, pp. 275–279. Kluwer Academic Publishers, Dordrecht.
Gianfranceschi, L., J.M. McDermott, N. Seglias, B. Koller, M. Kellerhals & C. Gessler, 1994. First results in developing a marker assisted breeding for resistance against scab. In: H. Schmidt and M. Kellerhals. Progress in temperate fruit breeding, pp. 291–294. Kluwer Academic Publishers, Dordrecht.
Giovanonni, J.J., R.A. Wing, M.W. Ganal & S.D. Tanksley, 1991. Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res. 19: 6553–6558.
Gogorcena, Y., S. Arulsekar, A.M. Dandekar & D.E. Parfitt, 1993. Molecular markers for grape characterization. Vitis 32: 183–185.
Grattapaglia, D., J. Chaparro, P. Wilcox, S. McCord, D. Wemer, H. Amerson, S. McKeand, F. Bridgewater, L. McIntyre, R. Doerge, B. Weir, R. Whetten, D. O'Malley & R. Sederoff, 1992a. RAPD mapping and tree improvement. Plant Genome I, San Diego Nov. 9–11, 1992:14.
Grattapaglia, D., J. Chaparro, P Wilcox, S. McCord, D. Werner, H. Amerson, S. McKeand, F Bridgewater, R. Whetten, D. O'Malley & R. Sederoff, 1992b. Mapping in woody plants with RAPD markers: Applications to breeding in forestry and horticulture. Application of RAPD technology to plant breeding, Joint plant breeding symposia series, Minneapolis Nov. 1, 1992: 37–40.
Harada, T., K. Matsuyama, T. Sato, R. Ishikawa, M. Niizeki & K. Saito, 1993. DNA-RAPDs detect genetic variation and paternity in Malus. Euphytica 65: 87–91.
Hemmat M., N.Y. Weeden, A.G. Manganaris & D.M. Lawson, 1994. Molecular marker linkage map for apple. J. Hered. 85: 4–11.
Hem, M. & T. Helentjaris, 1993. Inheritance of RAPDs in Fl hybrids of corn. Theor. Appl. Genet. 85: 961–968.
Hoepfner, A.-S., H. Nybom, U. Carlsson & R. Franzén, 1993. DNA fingerprinting useful for monitoring cell line identity in micro propagated raspberries. Acta Agricult. Scand., Sect. B 43: 53–57.
Jeanjaques, I., A. Defontaine & J.N. Hallet, 1993. Characterization ofVitis vinifera cultivars by random amplified polymorphic DNA markers. Vitis 32: 189–190.
Kaemmer, D., R. Afza, K. Weising, G. Kahl & F.J. Novak, 1992. Oligonucleotide and amplification fingerprinting of wild species and cultivars of banana (Musa spp.) Bio/Technology 10: 1030–1035.
King, G., 1994. The European apple genome mapping project - progress in mapping agronomic genes in apple. in: H. Schmidt and M. Kellerhals. Progress in temperate fruit breeding, pp. 263–267. Kluwer Academic Publishers, Dordrecht.
Koller, B., A. Lehmann, J.M. McDermott & C. Gessler, 1993. Identification of apple cultivars using RAPD markers. Theor. Appl. Genet. 85: 901–904.
Komatsu, A., T. Akihama, T. Hidaka & M. Omura, 1993. Identification ofPoncirus strains by DNA fingerprinting. In: T. Hayashi, M. Omura & N.S. Scott (Eds). Techniques on Gene Diagnosis and Breeding in Fruit Trees, pp. 88–95. Fruit tree research station, Ibaraki.
Lavi, U., J. Hillel, A. Vainstein, E. Lahav & D. Sharon, 1991. Application of DNA fingerprints for identification and genetic analysis of avocado. J. Amer. Soc. Hort. Sci. 116: 1078–1081.
Lodhi, M.A., B.I. Reisch & N.F. Weeden, 1992. Molecular genetic mapping and genome size ofVitis. Plant Genome I, San Diego Nov. 9–11, 1992: 37.
Martin, G.B., M.C. de Vicente & S.D. Tanksley, 1993. High-resolution linkage analysis and physical characterization of thePto bacterial resistance locus in tomato. Molecular Plant-Microbe Interactions 6: 26–34.
Matsuyama, T., R. Motohashi, T. Akihama & M. Omura, 1992. DNA fingerprinting in Citrus cultivars. Japan. J. Breed. 42: 155–159.
Matsuyama, T., M. Omura & T. Akihama, 1993. DNA fingerprinting in Citrus cultivars. In: T. Hayashi, M. Omura & N.S. Scott (Eds). Techniques on Gene Diagnosis and Breeding in Fruit Trees, pp. 26–30. Fruit tree research station, Ibaraki.
Michelmore, R.W., I. Paran & R.V. Kesseli, 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA 88: 9828–9832.
Miklas, P.N., J.R. Stavely & J.D. Kelly, 1993. Identification and possible use of a molecular marker for rust resistance in common bean. Theor. Appl. Genet. 85: 745–749.
Mulcahy, D.L., M. Cresti, S. Sansavini, G.C. Douglas, H.F. Linskens, G.B. Mulcahy, R. Vignani & M. Pancaldi, 1993. The use of random amplified polymorphic DNAs to fingerprint apple genotypes. Sci. Horticult. 54: 89–96.
Nybom, H., 1990a. Genetic variation in ornamental apple trees and their seedlings (Malus, Rosaceae) revealed by DNA ‘fingerprinting’. Hereditas 113: 17–28.
Nybom, H., 1990b. DNA fingerprints in sports of ‘Red Delicious’ apples. HortScience 25: 1641–1642.
Nybom, H., 1991. Applications of DNA fingerprinting in plant breeding. In: T. Burke, G. Dolf, A.J. Jeffreys & R. Wolff (Eds). DNA Fingerprinting: Approaches and Applications, pp. 294–311. Birkhäuser, Basel.
Nybom, H. & H.K. Hall, 1991. Minisatellite DNA “fingerprints” can distinguishRubus cultivars and estimate their degree of relatedness. Euphytica 53: 107–114.
Nybom, H. & B.A. Schaal, 1990. DNA “fingerprints” applied to paternity analysis in apples (Malus ×domestica). Theor. Appl. Genet. 79: 763–768.
Nybom, H., B.A. Schaal & S.H. Rogstad, 1989. DNA “fingerprints” can distinguish cultivars of blackberries and raspberries. 5th International Symposium onRubus & Ribes, Acta Horticult. 262: 305–310.
Nybom, H., S.H. Rogstad & B.A. Schaal, 1990. Genetic variation detected by use of the M13 “DNA fingerprint” probe inMalus, Prunus, andRubus (Rosaceae). Theor. Appl. Genet. 79: 153–156.
Nybom, H., U. Carlson & A.-S. Hoepfner, 1992. [The DNA laboratory at Balsgård]. Balsgård - Dept. Horticult. Plant Breeding, Swedish Univ. Agricult. Sci., Biennial Rept 1990–91: 146–154.
Nyman, M., 1993. Protoplast technology in strawberries. Acta Universitatis Upsaliensis, Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science, No. 451.
Paran, L., R. Kesseli & R. Michelmore, 1991. Identification of restriction fragment length polymorphism and random amplified polymorphic DNA markers linked to downy mildew resistance genes in lettuce, using near-isogenic lines. Genome 34: 1021–1027.
Parent, J.-G. & D. Pagé, 1992. Identification of raspberry cultivars by nonradioactive-DNA fingerprinting. HortScience 27: 1108–1110.
Parent, J.-G., M.G. Fortin & D. Pagé, 1993. Identification de cultivars de framboisier par l'analyse d'ADN polymorphe amplifié au hasard (RAPD). Can. J. Plant Sci. 73: 1115–1122.
Poulsen, G.B., G. Kahl & K. Weising, 1993. Abundance and polymorphism of simple repetitive DNA sequences inBrassica napus L. Theor. Appl. Genet. 85: 994–1000.
Poulsen, G.B., G. Kahl & K. Weising, 1993. Oligonucleotide fingerprinting of resynthesizedBrassica napus. Euphytica. 70: 53–59.
Rogstad, S.H., J.C. Patton II & B.A. Schaal, 1988. M13 repeat probe detects DNA minisatellite-like sequences in gymnosperms and angiosperms. Proc. Natl. Acad. Sci. USA 85: 9176–9178.
Ryskov, A.P., A.G. Jincharadze, M.I. Prosnyak, P.L. Ivanov & S.A. Limborska, 1988. M13 phage DNA as a universal marker for DNA fingerprinting of animals, plants and microorganisms. F.E.B.s Letters 233: 388–392.
Schmidt, T., K. Boblenz, M. Metzlaff, D. Kaemmer, K. Weising & G. Kahl, 1993. DNA fingerprinting in sugar beet (Beta vulgaris) - Identification of double-haploid breeding lines. Theor. Appl. Genet. 85: 653–657.
Sharon, D., J. Hillel, A. Vainstein & U. Lavi, 1992. Application of DNA fingerprints for identification and genetic analysis of Carica papaya and otherCarica species. Euphytica 62: 119–126.
Shimada, T., T. Haji & K. Hosaka, 1993. Classification and parent determination by RAPD in mume. In: T. Hayashi, M. Omura & N.S. Scott (Eds). Techniques on Gene Diagnosis and Breeding in Fruit Trees, pp. 77–80. Fruit tree research station, Ibaraki.
Sierotzki, H., M. Eggenschwiler, J. McDermott & C. Gessler, 1994. Specific virulence of isolates ofVenturia inaequalis on “susceptible” apple cultivars. EucarpiaFruit Breeding Sect. Meeting, Aug. 30–Sept. 3, 1993.
Stiles, J.I., C. Lemme, S. Sondur, M.B. Morshidi & R. Manshardt, 1993. Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars. Theor. Appl. Genet, 85: 697–701.
Striem, M.J., P. Spiegel-Roy, G. Ben-Hayyim, J. Beckmann & D. Gidoni, 1990. Genomic DNA fingerprinting ofVitis vinifera by the use of multi-locus probes. Vitis 29: 223–227.
Teramoto, S., Y. Kano-Murakami, M. Hori & K. Kamiyama, 1993. “DNA fingerprinting” can distinguish cultivar of Japanese pear. In: T. Hayashi, M. Omura & N.S. Scott (Eds). Techniques on Gene Diagnosis and Breeding in Fruit Trees, pp. 74–76. Fruit tree research station, Ibaraki.
Vosman, B., P. Arens, W. Rus-Kortekaas & M.J.M. Smulders, 1992. Identification of highly polymorphic DNA regions in tomato. Theor. Appl. Genet. 85: 239–244.
Waugh, R., E. Baird & W. Powell, 1992. The use of RAPD markers for the detection of gene introgression in potato. Plant Cell Reports 11: 466–469.
Weeden, M.E., G.M. Timmerman, M. Hemmat, B.E. Kneen & M.A. Lodhi, 1992. Inheritance and reliability of RAPD markers. Application of RAPD technology to plant breeding, Joint plant breeding symposia series, Minneapolis Nov. 1, 1992: 12–17.
Weeden, N.F., M. Hemmat, D.M. Lawson, M. Lohdi, R.L. Bell, A. Manganaris, B.I. Reisch, S.K. Brown & G.N. Ye, 1994. Development and application of molecular marker linkage maps in woody fruit crops. In: H. Schmidt and M. Kellerhals (Eds.) Progress in temperate fruit breeding, pp. 269–273. Kluwer Academic Publishers, Dordrecht.
Weising, K., J. Ramser, D. Kaemmer, G. Kahl & J.T. Epplen, 1991. Oligonucleofide fingerprinting in plants and fungi. In: T. Burke, G. Dolf, A.J. Jeffreys & R. Wolff (Eds). DNA Fingerprinting: Approaches and Applications, pp. 313–329. Birkhäuser, Basel.
Weising, K., D. Kaemmer, F. Weigand, J.T. Epplen & G. Kahl, 1992. Oligonucleotide fingerprinting reveals various probe-dependent levels of informativeness in chickpea (Cicer arietinum). Genome 35:436–442.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18: 6231–6235.
Wong, Z., V. Wilson, I. Patel, S. Povey & A.J. Jeffreys, 1987. Characterization of a panel of highly variable minisatellites cloned from human DNA. Ann. Hum. Genet. 51: 269–288.
Xu, Y.-S., M.S. Clark & E. Pehu, 1993. Use of RAPD markers to screen somatic hybrids betweenSolanum tuberosum andS. brevidens. Plant Cell Reports 12: 107–109.
Yang, H. & J. Krüger,1994. Identification of a RAPD marker linked to theVf gene for scab resistance in apples. Plant Breeding, in press.
Yang, H. & H. Schmidt, 1994. Selection of a mutant from adventitious shoots formed in X-ray treated cherry leaves and differentiation of standard and mutant with RAPDs. In: H. Schmidt and M. Kellerhals (Eds.) Progress in temperate fruit breeding, pp. 287–290. Kluwer Academic Publishers, Dordrecht.
Zhao, X.P. & G. Kochert, 1993. Phylogenetic distribution and genetic mapping of a (GGC)( n ) microsatellite from rice (Oryza sativa). Plant Molec. Biol.21: 607–614.
Zischler, H., A. Hinkkanen & R. Studer, 1991. Oligonucleotide fingerprinting with (CAC)5: Nonradioactive in-gel hybridization and isolation of individual hypervariable loci. Electrophoresis 12: 141–146.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nybom, H. DNA fingerprinting — A useful tool in fruit breeding. Euphytica 77, 59–64 (1994). https://doi.org/10.1007/BF02551462
Issue Date:
DOI: https://doi.org/10.1007/BF02551462