Skip to main content
Log in

Electrically induced neurite outgrowth of PC12 cells on the electrode surface

  • Cellular Engineering
  • Published:
Medical and Biological Engineering and Computing Aims and scope Submit manuscript

Abstract

Morphological differentiation of PC12 cells cultured on an indium-tin oxide (ITO) electrode has been induced to grow neurites in the absence of nerve growth factor (NGF) by electrical stimulation. Rectangular pulse wave potentials were applied to the electrode at amplitudes of 200 mV and 400 mV with frequencies of 50Hz, 500Hz, and 1 kHz. The PC12 cells differentiated most prominently at 200 mV with 100Hz. No statistically significant differences were observed among the electrically induced neurite lengths. The electrically induced differentiation was completely inhibited by a blockade of calcium influx using LaCl3. This indicates that repeated potential shift in the vicinity of a cellular membrane may stimulate morphological response, probably through calcium ion channels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arcangeli, A., Becchetti, A., Mannini, A., Mugnai, G., De Filippi, P., Tarone, G., Del Bene, M. R., Barletta, E., Wanke, E. andOlivotto, M. (1993): ‘Integrin-mediated neurite outgrowth in neuroblastoma cells depends on the activation of potassium channels’,J. Cell. Biol.,122, pp. 1131–1143

    Article  Google Scholar 

  • Cavalie, A., Berninger, B., Haas, C. A., Garcia, D. E., Lindholm, D. andLux, H. D. (1994): ‘Constitutive upregulation of calcium channel currents in rat phaeochromocytoma cells: role of c-fos and c-jun’,J. Physiol. (Lond),479, pp. 11–27

    Google Scholar 

  • Curran, T. andMorgan, J. I. (1986): ‘Barium modulates c-fos expression and post-translational modification’,Proc. Natl. Acad. USA,83, pp. 8521–8524

    Article  Google Scholar 

  • Garyantes, T. K. andRegehr, W. G. (1992): ‘Electrical activity increases growth cone calcium but fails to inhibit neurite out-growth from rat sympathetic neurons’,J. Neurosci.,12, pp. 96–103

    Google Scholar 

  • Greenberg, M. E., Greene, L. A. andZiff, E. B. (1985): ‘Nerve growth factor and epidermal growth factor induced rapid transient changes in proto-oncogene transcription in PC12 cells’,J. Biol. Chem.,260, pp. 14101–14110

    Google Scholar 

  • Greenberg, M. E., Ziff, E. B. andGreene, L. A. (1986): ‘Stimulation of neuronal acetylcholine receptors induces rapid gene transcription’,Science,234, pp. 80–83

    Article  Google Scholar 

  • Greene, L. A. andTischler, A. S. (1976): ‘Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor’,Proc. Natl. Acad. Sci. USA,73, pp. 2424–2428

    Article  Google Scholar 

  • Ishida, T., Peterson, T. E., Kovach, N. L. andBerk, B. C. (1996): ‘Map kinase activation by flow in endothelial cells. Role of beta 1 integrins and tyrosine kinases’,Ciric. Res.,79, pp. 310–316

    Google Scholar 

  • Kojima, J., Shinohara, H., Ikariyama, Y., Aizawa, M., Nagaike, K. andMorioka, S. (1991): ‘Electrically controlled proliferation of human carcinoma cells cultured on the surface of an electrode’,J. Biotechnol.,18, pp. 129–139

    Article  Google Scholar 

  • Kojima, J., Shinohara, H., Ikariyama, Y. andAizawa, M. (1992): ‘Electrically promoted protein production by mammalian cells cultured on the electrode surface’,Biotechnol. Bioeng.,39, pp. 27–32

    Article  Google Scholar 

  • Koyama, S., Haruyama, T., Kobatake, E. andAizawa, M. (1997): ‘Electrically induced NGF production by astroglial cells’,Nature Biotechnol.,15, pp. 164–166

    Article  Google Scholar 

  • Koyama, S., Yanagida, Y., Haruyama, T., Kobatake, E. andAizawa, M. (1996): ‘Molecular mechanisms of electrically stimulated NGF expression and secretion by astrocytes cultures on the potential controlled electrode surface’,Cell. Eng.,1, pp. 189–194

    Google Scholar 

  • Langton, P. D. (1993): ‘Calcium channel currents recorded from isolated myocytes of rat basilar artery are stretch sensitive’,J. Physiol. (Lond),471, pp. 1–11

    Google Scholar 

  • Manivannan, S. andTerakawa, S. (1993): ‘Rapid filopodial sprouting induced by electrical stimulation in nerve terminals’,Jpn. J. Physiol.,43 (suppl. 1), pp. 217–220

    Google Scholar 

  • Messing, R. O., Stevens, A. M., Kiyasu, E. andSneade, A. B. (1989): ‘Nicotinic and muscarinic agonists stimulate rapid protein kinase C translocation in PC12 cells’,J. Neurosci.,9, pp. 507–12

    Google Scholar 

  • Mie, M., Ohgushi, H., Haruyama, T., Kobatake, E. andAizawa, M. (1996): ‘Electrically enhanced osteogenic differentiation of rat bone marrow stromal stem cells’,Cell. Eng.,1, pp. 153–158

    Google Scholar 

  • Miyamoto, S., Teramoto, H., Coso, O. A., Gutkind, J. S., Burbelo, P. D., Akiyama, S. K. andYamada, K. M. (1995): ‘Integrin function: molecular hierarchies of cytoskeletal and signalling molecules’,J. Cell. Biol.,131, pp. 791–805

    Article  Google Scholar 

  • Morgan, J. I. andCurran, T. (1986): ‘Role of ion flux in the control of c-fos expression’,Nature,322 pp. 552–555

    Article  Google Scholar 

  • Rosen, L. B. andGreenberg, M. E. (1996): ‘Stimulation of growth factor receptor signal transduction by activation of volatage-sensitive calcium channels’,Proc. Natl. Acad. Sci. USA,93, pp. 1113–1118

    Article  Google Scholar 

  • Rusanescu, G., Qi, H., Thomas, S. M., Brugge, J. S. andHalegoua, S. (1995): ‘Calcium influx induces neurite growth through a Src-Ras signalling cassette’,Neuron,15, pp. 1415–1425

    Article  Google Scholar 

  • Schaller, M. D. andParsons, J. T. (1994): ‘Pocal adhesion kinase and associated proteins’,Curr. Opin. Cell Biol.,6, pp. 705–710

    Article  Google Scholar 

  • Sheng, M., Dougan, S. T., McFadden, G. andGreenberg, M. E. (1988): ‘Calcium and growth factor pathways of c-fos transcriptional activation require distinct upstream regulatory sequences’,Mol. Cell. Biol.,8, pp. 2787–2796

    Google Scholar 

  • Taniguchi, J. andGuggino, W. B. (1989): ‘Membrane stretch: a physiological stimulator of Ca2+-activated K+ channels in thick ascending limb’,Am. J. Physiol.,257, pp. F347-F352

    Google Scholar 

  • Thomas, S. M., DeMarco, M., D'Arcangelo, G., Halegoua, S. andBrugge, J. S. (1992): ‘Ras is essential for nerve growth factor- and phorbol ester-induced tyrosine phosphorylation of MAP kinases’,Cell,68, pp. 1031–40

    Article  Google Scholar 

  • Traynor, A. E. andSchubert, D. (1984): ‘Phospholipases elevate cyclic AMP levels and promote neurite extension in a clonal nerve cell line’,Dev. Brain Res.,14, pp. 197–204

    Google Scholar 

  • Wood, K. W., Sarnecki, C., Roberts, T. M. andBlenis, J. (1992): ‘Ras mediates nerve growth factor receptor modulation of three signal-transducing protein kinases: MAP kinase, Raf-1, and RSK’,Cell,68, pp. 1041–50

    Article  Google Scholar 

  • Zwick, E., Daub, H., Aoki, N., Yamaguchi-Aoki, Y., Tinhofer, I., Maly, K. andUllrich, A. (1997): ‘Critical role of calcium-dependent epidermal growth factor receptor transactivation in PC12 cell memrane depolarization and bradykinin signalling’,J. Biol. Chem.,272, pp. 24767–24770

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. Aizawa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kimura, K., Yanagida, Y., Haruyama, T. et al. Electrically induced neurite outgrowth of PC12 cells on the electrode surface. Med. Biol. Eng. Comput. 36, 493–498 (1998). https://doi.org/10.1007/BF02523221

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02523221

Keywords

Navigation