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Purification of a cell wall-bound pectin-gelatinizing factor and examination of its identity with pectin methylesterase

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Abstract

A cell wall-bound proteinous factor which causes the gelation of apple pectin solution was examined as to whether it is identical with pectin methylesterase or not. Gel filtration and chromatographic analyses with columns of isolated cell walls and CM Sephadex strongly suggested their identity. The factor caused demethylation of the pectin.

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Abbreviations

NPP:

disodiump-nitrophenylphosphate

PGF:

pectin-gelatinizing factor

PME:

pectin methylesterase

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Authors and Affiliations

  1. Laboratory of Science Education Research, Faculty of Education, Akita University, Tegata-Gakuen-Cho 1-1, 010, Akita City, Akita

    Tsuyoshi Yamaoka, Kazue Tsukada, Hiroko Takahashi & Naoko Yamauchi

Authors
  1. Tsuyoshi Yamaoka
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  2. Kazue Tsukada
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  3. Hiroko Takahashi
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  4. Naoko Yamauchi
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Yamaoka, T., Tsukada, K., Takahashi, H. et al. Purification of a cell wall-bound pectin-gelatinizing factor and examination of its identity with pectin methylesterase. Bot Mag Tokyo 96, 139–144 (1983). https://doi.org/10.1007/BF02491100

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  • DOI: https://doi.org/10.1007/BF02491100

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