Summary
The aim of this investigation was the study and development of analytical procedures suitable for the assay of glutathione (GSH) in pharmaceutical formulations. Two are based on isocratic HPLC with a 250 mm×4.6 mm i.d., 5 μm C18 column and UV detection. In the first procedure sample solutions were injected without pretreatment whereas in the second the samples were injected after derivatization with Ellman’s reagent which forms an easily detectable adduct with GSH. Good linearity was obtained over the range 0.12–6.00×10−4M for the direct procedure and 0.25–3.00×10−4M for the derivatization procedure. The precision and rapidity of analysis were also good for both methods. The third method is based on capillary zone electrophoresis (CZE) in a 27 cm×75 μm i.d untreated fused silica capillary containing pH 7 phosphate buffer. All results are in good agreement with a spectrophotometric procedure used as reference method.
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Raggi, M.A., Mandrioli, R., Bugamelli, F. et al. Comparison of analytical methods for quality control of pharmaceutical formulations containing glutathione. Chromatographia 46, 17–22 (1997). https://doi.org/10.1007/BF02490925
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DOI: https://doi.org/10.1007/BF02490925