Skip to main content
SpringerLink
Log in

Reversed-phase high-performance liquid chromatographic separation of mono- and divinyl chlorophyll forms using pyridine-containing mobile phases and a polymeric octadecylsilica column

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

The separation of chlorophyll forms was studied employing a wide bore polymeric octadecylsilica column and pyridine containing mobile phases, giving consideration to considering the influence of mobile phase composition and column temperature on the resolution of monovinyl forms from their divinyl analogues. A method involving gradient elution and operating at 15°C is proposed for the separation of several polar and non-polar mono- and divinyl chlorophylls from etiolated tissues of higher plants and from marine phytoplankton. The advantages of pyridine as a mobile phase additive in the reversed-phase liquid chromatography of chlorophylls are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. A. Rebeiz, R. Parham, D. A. Fasoula, I. M. Ioannides, in “The Biosynthesis of Tetrapyrrole Pigments,”, Ciba Foundation Symposium 180 John Wiley & Sons, Chichester 1994, p. 177.

    Google Scholar 

  2. C. A. Rebeiz, J. Lascelles, in “Photosynthesis. Vol. I: Energy Conversion by Plants and Bacteria,” Govindjee, ed., Academic Press, New York, 1982, p. 699.

    Google Scholar 

  3. Y. Shioi, K. Takamiya, Plant Physiol.100, 1291 (1992).

    CAS  Google Scholar 

  4. S. W. Jeffrey, in “The Chromophyte Algae: Problems and Perspectives,” J. C. Green, B. S. C. Leadbetear and W. L. Diver, eds., Clarendon Press, Oxford, 1989, p. 13.

    Google Scholar 

  5. R. R. Bidigare, M. C. Kennicutt, M. E. Ondrusek, M. D. Keller, R. R. L. Guillard, Energy Fuels,4, 65 (1990).

    Article  Google Scholar 

  6. J. L. Garrido, M. Zapata, S. Muñiz, J. Phycol.31, 761 (1995).

    Article  CAS  Google Scholar 

  7. S. W. Chisholm, S. L. Frankel, R. Goericke, R. J. Olson, B. Palenik, J. B. Waterbury, L. West-Johnrud, E. R. Zettler, Arch. Microbiol.,157, 297 (1992).

    Article  CAS  Google Scholar 

  8. R. Goericke, D. J. Repeta, Mar. Ecol. Prog Ser.101, 307 (1993).

    CAS  Google Scholar 

  9. I. M. Ioannides, K. R. Robertson, C. A. Rebeiz, Plant Physiol. (Life Sci. Adv.),11, 79 (1992).

    Google Scholar 

  10. S. W. Jeffrey, in “Primary Productivity in the Sea,” P. G. Falkowski, ed., Plenum Publishing Corporation, New York, 1980, p. 33.

    Google Scholar 

  11. K. S. Rowan, “Photosynthetic Pigments of Algae” Cambridge University Press, New York, 1989, p. 66.

    Google Scholar 

  12. D. F. Millie, H. W. Paerl, J.P. Hurley, Can J. Fish. Aquat. Sci.50, 2513 (1993).

    CAS  Google Scholar 

  13. E. J. H. Head, L. R. Harris, Mar Ecol. Prog. Ser86, 229 (1992).

    CAS  Google Scholar 

  14. Y. Shioi, S. I. Beale, Anal. Biochem.162, 493 (1987).

    Article  CAS  Google Scholar 

  15. B. J. Whyte, W. T. Griffiths, Biochem. J.291, 939 (1993).

    CAS  Google Scholar 

  16. S. W. Wright, S. W. Jeffrey, R. F. C. Mantoura, C. A. Llewellyn, T. Biornland, D. Repeta, N. Welschmeyer, Mar. Ecol. Prog. Ser.77, 183 (1991).

    CAS  Google Scholar 

  17. L. Van Heukelem, A. J. Lewitus, T. M. Kana, N. E. Craft, J. Phycol.28, 867 (1992).

    Article  Google Scholar 

  18. Y. Shioi, R. Fukae, T. Sasa, Biochim. Biophys. Acta722, 72 (1983).

    Article  CAS  Google Scholar 

  19. N. Suzuki, K. Saitoh, K. Adachi, J. Chromatogr.408, 181 (1987).

    Article  CAS  Google Scholar 

  20. P. Barry, A. J. Young, G. Britton, J. Exp. Bot.42, 229 (1991).

    CAS  Google Scholar 

  21. S. O. Duke, M. V. Duke, H. J. Lee, Z. Naturforsch48c, 317 (1993).

    Google Scholar 

  22. B. Schoefs, M. Bertrand, Y. Lemoine, J. Chromatogr. A692, 239 (1995).

    Article  CAS  Google Scholar 

  23. J. L. Garrido, M. Zapata, J. High Resolut. Chromatogr16, 229 (1993).

    Article  CAS  Google Scholar 

  24. J. L. Garrido, M. Zapata, Chromatographia35, 543 (1993).

    Article  CAS  Google Scholar 

  25. Y. Shioi, K. Watanabe, K. Takamiya, J. L. Garrido, M. Zapata, Anal. Biochem.231, 225 (1995).

    Article  CAS  Google Scholar 

  26. L. C. Sander, S. A. Wise, Anal. Chem.61, 1749 (1989).

    Article  CAS  Google Scholar 

  27. L. Van Heukelem, A. J. Lewitus, T. M. Kana, N. E. Craft, Mar. Ecol. Prog. Ser.114, 303 (1994).

    Google Scholar 

  28. K. Van Lenning, J. L. Garrido, J. Aristegui, M. Zapata, Chromatographia41, 539 (1995).

    Article  Google Scholar 

  29. M. Zapata, A. M. Ayala, J. M. Franco, J. L. Garrido, Chromatographia23, 26 (1987).

    Article  CAS  Google Scholar 

  30. J. L. Garrido, M. Zapata, J. Chromatogr. A738, 285 (1996).

    Article  CAS  Google Scholar 

  31. C. M. Hanamoto, P. A. Castelfranco, Plant. Physiol73, 79 (1983).

    Article  CAS  Google Scholar 

  32. M. Zapata, J. L. Garrido, J. Phycol. (submitted)

  33. L. C. Sander, S. A. Wise, J. Chromatogr. A656, 335 (1993).

    Article  CAS  Google Scholar 

  34. J. L. Garrido, M. Zapata, J. Phycol. (submitted).

  35. M. W. Fawley, J. Phycol.25, 601 (1989).

    CAS  Google Scholar 

  36. S. Budavari (editor), “The Merck Index. Centennial Edition”, Merck, Rahway, 1989, p. 7984.

    Google Scholar 

  37. L. R. Snyder, J. J. Kirkland, “Introduction to Modern Liquid Chromatography. Second Edition,” John Wiley and Sons, New York, 1979, p. 249.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Investigacions Mariñas, CSIC, Av. Eduardo, Cabello 6, 36208, Vigo, Spain

    J. L. Garrido

  2. Centro de Investigacions Mariñas, Consellería de Pesca, Xunta de Galicia, Apdo. 208, 36600, Vilagarcía de Arousa, Spain

    M. Zapata

Authors
  1. J. L. Garrido
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Zapata
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Garrido, J.L., Zapata, M. Reversed-phase high-performance liquid chromatographic separation of mono- and divinyl chlorophyll forms using pyridine-containing mobile phases and a polymeric octadecylsilica column. Chromatographia 44, 43–49 (1997). https://doi.org/10.1007/BF02466514

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02466514

Key Words

Search

Navigation