Skip to main content
SpringerLink
Log in

Effect of activation of Ca2+, Mg2+-dependent endonuclease on proliferative response of human peripheral blood lymphocytes

  • Biophysics and Biochemistry
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

Activity of Ca2+, Mg2+-dependent endonuclease in human peripheral blood mononuclears is studied. Intact cells exhibit extremely low activity of the enzyme. Treatment with the synthetic hexapeptide imunofan considerably stimulates endonuclease activity in mononuclears. This activation does not depend on additional protein synthesis. An 1-h incubation in the presence of cycloheximide also activates Ca2+, Mg2+-dependent endonuclease. These data suggest that imunofant and/or cycloheximide activate the apoptotic cascade. This leads to activation of endonuclease, which is not synthesizedde novo but persists in the cell in the form of inactive precursor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. V. M. Pisarev, A. V. Tutel'yan, A. V. Danilina,et al., Vopr. Med. Khimii,41, No. 2, 11–15 (1995).

    PubMed  CAS  Google Scholar 

  2. T. I. Tkacheva, S. S. Kirson, N. N. Khodarev, and I. I. Votrin,Byull. Eksp. Biol. Med.,109, No. 5, 543–546 (1990).

    CAS  Google Scholar 

  3. N. N. Khodarev, T. N. Ivanova, and I. I. Votrin,Dokl. Akad. Nauk SSSR,268, 1000–1003 (1983).

    PubMed  CAS  Google Scholar 

  4. J. J. Cohen and R. C. Duke,J. Immunol.,132, 38–42 (1984).

    PubMed  CAS  Google Scholar 

  5. D. R. Hewish and L. A. Burgoyne,Biochem. Biophys. Res. Commun.,52, 504–510 (1973).

    Article  PubMed  CAS  Google Scholar 

  6. K. Guido and M. A. Karlos,Immunol. Today,5, No. 5, 235–240 (1994).

    Google Scholar 

  7. M. A. Nieto and A. Lopez-Rivas,J. Immunol.,143, No. 12, 4166–4170 (1989).

    PubMed  CAS  Google Scholar 

  8. I. A. Sokolova, A. U. Volgin, N. V. Makarova,et al., FEBS Lett.,313, No. 3, 295–296 (1992).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medicogenetic Research Center, Russian Academy of Medical Sciences, Moscow

    G. V. Shmarina & E. L. Zyuzina

Authors
  1. G. V. Shmarina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. L. Zyuzina
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 123, No. 5, pp. 535–537, May, 1997

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shmarina, G.V., Zyuzina, E.L. Effect of activation of Ca2+, Mg2+-dependent endonuclease on proliferative response of human peripheral blood lymphocytes. Bull Exp Biol Med 123, 465–467 (1997). https://doi.org/10.1007/BF02445320

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02445320

Key words

Search

Navigation