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New lights in early steps of in vitro fertilization in plants

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Abstract

Studies using in vitro fertilization systems in animals and lower plants have led to a better understanding of the initial steps of fertilization and their underlying mechanisms. These mechanisms remain to be elucidated in flowering plants. Recent progress related to the development of in vitro fertilization systems using maize as a plant model is presented in this review. Their potential for leading to a better understanding of the process of gametic recognition and fusion and of the early events triggering egg activation and zygote formation are also discussed.

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References

  • Brawley SH (1991) The fast block against polyspermy in fucoid algae is an electrical block. Dev Biol 144:94–106

    Article  PubMed  CAS  Google Scholar 

  • Breton C, Faure JE, Dumas C (1995) From in vitro fertilization to early embryogenesis in maize. Protoplasma 187:3–12

    Article  CAS  Google Scholar 

  • Brownlee C (1994) Signal transduction during fertilisation in algae and vascular plants. New Phytol 127:399–423

    Article  Google Scholar 

  • Callow ME, Stafford CJ, Green JR (1992) Gamete recognition and fertilisation in the fucoid algae. In: Callow JA, Green JR (eds) Perspectives in plant cell recognition. Cambridge University Press, Cambridge, pp 19–31

    Google Scholar 

  • Carlson WR (1986) The B chromosome of maize. Crit Rev Plant Sci 3:201–226

    Google Scholar 

  • Chaboud A, Perez R (1992) Generative cells and male gametes: isolation, physiology, and biochemistry. Int Rev Cytol 140:205–232

    CAS  Google Scholar 

  • Chaboud A, Perez R, Digonnet C, Dumas C (1992) Gamete recognition in angiosperms: model and strategy for analysis. In: Callow JA, Green JR (eds) Perspectives in plant cell recognition. Cambridge University Press, Cambridge, pp 59–77

    Google Scholar 

  • Chandler DE (1991) Multiple intracellular signals coordinate structural dynamics in the sea urchin egg cortex at fertilization. J Electron Microsc 17:266–293

    Article  CAS  Google Scholar 

  • Chasan R (1992). Frontiers in fertilization. Plant Cell 4:369–371

    Article  PubMed  Google Scholar 

  • Dresselhaus T, Lörz H, Kranz E (1994) Representative cDNA libraries from few plant cells. Plant J 5:605–610

    PubMed  CAS  Google Scholar 

  • Dresselhaus T, Hagel C, Lörz H, Kranz E (1996) Isolation of a full-length cDNA encoding calreticulin from a PCR library of in vitro zygotes of maize. Plant Mol Biol 31:23–34

    Article  PubMed  CAS  Google Scholar 

  • Dumas C, Faure JE (1995) Use of in vitro fertilization and zygote culture in crop improvement. Curr Opin Biotech 6:183–188

    Article  CAS  Google Scholar 

  • Dumas C, Mogensen HL (1993) Gametes and fertilization: Maize as a model system for experimental embryogenesis in flowering plants. Plant Cell 5:1337–1348

    Article  PubMed  Google Scholar 

  • Duquis I, Roeckel P, Matthys-Rochon E, Dumas C (1987) Procedure to isolate viable sperm cells from corn (Zea mays L.) pollen grains. Plant Physiol 85:876–878

    Google Scholar 

  • Epel D (1990) The initiation of development at fertilization. Cell Differ Dev 29:1–12

    Article  PubMed  CAS  Google Scholar 

  • Faure JE, Mogensen HL, Kranz E, Digonnet C, Dumas C (1992) Ultrastructural characterization and three-dimensional reconstruction of isolated maize (Zea mays L.) egg cell protoplasts. Protoplasma 171:97–103

    Article  Google Scholar 

  • Faure JE, Mogensen HL, Dumas C, Lörz H, Kranz E (1993) Karyogamy after electrofusion of single egg and sperm cell protoplasts from maize: cytological evidence and time course. Plant Cell 5:747–755

    Article  PubMed  Google Scholar 

  • Faure JE, Digonnet C, Dumas C (1994a) An in vitro system for adhesion and fusion of maize gametes. Science 263:1598–1600

    PubMed  Google Scholar 

  • Faure JE, Digonnet C, Mòl R, Matthys-Rochon E, Dumas C (1994b) In vitro pollination and fertilisation in maize (Zea mays L.): technical procedures and prospects for the dissection of the double fertilisation process. Plant Sci 104:1–10

    Article  CAS  Google Scholar 

  • Faure JE, Aldon D, Rougier M, Dumas C (in press) Emerging data on pollen tube growth and fertilization in flowering plants: 1990–1995. Protoplasma

  • Gagliardi D, Breton C, Chaboud A, Vergne P, Dumas C (1995) Expression of heat shock factor and heat shock protein 70 genes during maize pollen development. Plant Mol Biol 29:841–856

    Article  PubMed  CAS  Google Scholar 

  • Guignard L (1899) Sur les anthérozoïdes et la double copulation sexuelle chez les végétaux angiospermes. Rev Gen Bot 11:129–135

    Google Scholar 

  • Jaffe LA, Cross NL (1986) Electrical regulation of sperm-egg fusion. Annu Rev Physiol 48:191–200

    Article  PubMed  CAS  Google Scholar 

  • Jaffe LF (1993) Classes and mechanisms of calcium waves. Cell Calcium 14:736–745

    Article  PubMed  CAS  Google Scholar 

  • Kranz E, Dresselhaus T (1996) In vitro fertilization with isolated higher plant gametes. Trends Plant Sci 1:82–89

    Article  Google Scholar 

  • Kranz E, Lörz H (1990) Micromanipulation and in vitro fertilization with single pollen grains of maize. Sex Plant Reprod 3:160–169

    Article  Google Scholar 

  • Kranz E, Lörz H (1993) In vitro fertilization with isolated, single gametes results in zygotic embryogenesis and fertile maize plants. Plant Cell 5:739–743

    Article  PubMed  Google Scholar 

  • Kranz E, Lörz H (1994) In vitro fertilisation of maize by single egg and sperm cell protoplast fusion mediated by high calcium and high pH. Zygote 2:125–128

    PubMed  CAS  Google Scholar 

  • Kranz E, Bautor J, Lörz H (1991a) In vitro fertilization of single, isolated gametes of maize mediated by electrofusion. Sex Plant Reprod 4:12–16

    Google Scholar 

  • Kranz E, Bautor J, Lörz H (1991b) Electrofusion-mediated transmission of cytoplasmic organelles throught the in vitro fertilization process, fusion of sperm cells with synergids and central cells, and cell reconstruction in maize. Sex Plant Reprod 4:17–21

    Google Scholar 

  • Kranz E, Wiegen P von, Lörz H (1995) Early cytological events after induction of cell division in egg cells and zygote development following in vitro fertilization with angiosperm gametes. Plant J 8:9–23

    Article  Google Scholar 

  • Kropf DL (1994) Cytoskeletal control of cell polarity in a plant zygote. Dev Biol 165:361–371

    Article  PubMed  CAS  Google Scholar 

  • Leduc N, Matthys-Rochon E, Rougier M, Mogensen L, Holm P, Magnard JL, Dumas C (in press) Isolated maize zygotes mimic in vivo embryonic development and express microinjected genes when cultured in vitro. Dev Biol

  • Nawaschin SG (1898), Resultate einer revision der befruchtungsvorgange beiLilium martagon undFritillaria tenella. Bull Acad Imp Sci St Petersburg 33:39–47

    Google Scholar 

  • Richert J, Kranz E, Lörz H, Dresselhaus T (1996) A reverse transcriptase-polymerase chain reaction assay for gene expression studies at the single cell level. Plant Sci 114:93–99

    Article  CAS  Google Scholar 

  • Roberts SK, Brownlee C (1995) Calcium influx, fertilisation potential and egg activation inFucus serratus. Zygote 3:191–197

    PubMed  CAS  Google Scholar 

  • Roberts SK, Gillot I, Brownlee C (1994) Cytoplasmic calcium andFucus egg activation. Development 120:155–163

    CAS  Google Scholar 

  • Roeckel P, Dumas C (1993) Survival at 20°C and cryopreservation of isolated sperm cells fromZea mays pollen grains. Sex Plant Reprod 6:212–216

    Article  Google Scholar 

  • Roman H (1948) Directed fertilization in maize. Proc Natl Acad Sci USA 34:46–52

    Article  Google Scholar 

  • Russell SD (1992) Double fertilization. Int Rev Cytol 140:357–388

    Article  Google Scholar 

  • Schatten G (1982) Motility during fertilization Int Rev Biol 79:59–163

    CAS  Google Scholar 

  • Schatten G (1994) The centrosome and its mode of inheritance: the reduction of the centrosome during gametogenesis and its restoration during fertilization. Dev Biol 165:299–335

    Article  PubMed  CAS  Google Scholar 

  • Shi L, Zhu T, Mogensen HL, Keim P (1996) Sperm identification in maize by fluorescence in situ hybridization. Plant Cell 8:815–821

    Article  PubMed  CAS  Google Scholar 

  • Snell WJ, White JM (1996) The molecules of mammalian fertilization. Cell 85:629–637

    Article  PubMed  CAS  Google Scholar 

  • Tirlapur UK, Kranz E, Cresti M (1995) Characterisation of isolated egg cells, in vitro products and zygotes ofZea mays L. using the technique of image analysis and confocal, laser scanning microscopy. Zygote 3:57–64

    PubMed  CAS  Google Scholar 

  • Vacquier VD (1981) Dynamic changes of the egg cortex. Dev Biol 84:1–26

    Article  PubMed  CAS  Google Scholar 

  • Wagner VT, Song YC, Matthys-Rochon E, Dumas C (1988) The isolated embryo sac ofZea mays: ultrastructural observations. In: Cresti M, Gori P, Pacini E (eds) Sexual reproduction in higher plants Springer, New York Berlin Heidelberg, pp 125–130

    Google Scholar 

  • Wagner VT, Song YC, Matthys-Rochon E, Dumas C (1989) Observations on the isolated embryo sac ofZea mays L. Plant Sci 59:127–132

    Article  Google Scholar 

  • Zhang G, Williams CM, Campenot MK, McGann LE, Cass DD (1992) Improvement of longevity and viability of sperm cells isolated from pollen ofZea mays L. Plant Physiol 100:47–53

    Article  PubMed  CAS  Google Scholar 

  • Zhang G, Gifford DJ, Cass DD (1993) RNA and protein synthesis in sperm cells isolated fromZea mays L. pollen. Sex Plant Reprod 6:239–243

    Article  Google Scholar 

  • Zhang G, Williams CM, Campenot MK, McGann LE, Cutler AJ, Cass DD (1995) Effects of calcium, magnesium potassium, and boron on sperm cells isolated from pollen ofZea mays L. Sex Plant Reprod 8:113–122

    Article  Google Scholar 

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Correspondence to M. Rougier.

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Rougier, M., Antoine, A.F., Aldon, D. et al. New lights in early steps of in vitro fertilization in plants. Sexual Plant Reprod 9, 324–329 (1996). https://doi.org/10.1007/BF02441950

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  • DOI: https://doi.org/10.1007/BF02441950

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