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Cloning of a mercuric ion-resistance operon intoEscherichia coli 5k using the mini-plasmid technique

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Biotechnology Techniques

Summary

Mercury resistant transformant clone ofEscherichia coli 5K bearing the recombinant mini-plasmid pHg (13 MDa) was obtained after treatment of the conjugative 60 MDa R plasmid pBH100 (from wild strainE. coli) withPstI followed by fragment ligation (ligase).

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Authors and Affiliations

  1. Departamento de Biologia Geral, UFMG, Caixa Postal 2486, Belo Horizonte, 31270, MG, Brazil

    A. M. A. Nascimento & E. Chartone-Souza

  2. Departamento de Biologia Celular, UnB, 70000, Brasília, DF, Brazil

    M. O. Azevedo & S. Astolfi-Filho

Authors
  1. A. M. A. Nascimento
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  2. M. O. Azevedo
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  3. S. Astolfi-Filho
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  4. E. Chartone-Souza
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Nascimento, A.M.A., Azevedo, M.O., Astolfi-Filho, S. et al. Cloning of a mercuric ion-resistance operon intoEscherichia coli 5k using the mini-plasmid technique. Biotechnol Tech 6, 139–142 (1992). https://doi.org/10.1007/BF02438820

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  • DOI: https://doi.org/10.1007/BF02438820

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