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Trans-esterification of fatty acids from microorganisms and human blood serum by trimethylsulfonium hydroxide (TMSH) for GC analysis

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Summary

Trimethylsulfonium hydroxide (TMSH) can convert fatty acids into the corresponding fatty acid methyl esters (FAMEs) in a single step. These fatty acids may also be bound in biomolecules such as phospholipids and/or glycerides. Complex mixtures of saturated and unsaturated FAMEs which may contain hydroxy and cylopropyl groups are obtained by trans-esterification; they can easily be separated in most cases by capillary GC. When FAMEs are generated from different microorganisms e.g. bacteria the patterns of the chromatograms are characteristic. Examples of characteristic patterns of bacteria with different cell wall structures are shown. The described method of transesterification can also be applied directly to blood serum without sophisticated sample pretreatment. The profiles of the chromatograms match well those described in the literature obtained by other methods of trans-esterification or sample preparation.

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Authors and Affiliations

  1. Institut für Medizinische Mikrobiologie, Universität-Gesamthochschule (GHS) Essen, Hufelandstr. 55, D-4300, Essen, FRG

    K.-D. Müller & H. P. Nalik

  2. Abteilung Chromatographie, Max-Planck-Institut für Kohlenforschung, Kaiser-Wihelm Platz 1, D-4330, Mülheim an der Ruhr, FRG

    H. Husmann & G. Schomburg

Authors
  1. K.-D. Müller
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  2. H. Husmann
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  3. H. P. Nalik
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  4. G. Schomburg
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Müller, KD., Husmann, H., Nalik, H.P. et al. Trans-esterification of fatty acids from microorganisms and human blood serum by trimethylsulfonium hydroxide (TMSH) for GC analysis. Chromatographia 30, 245–248 (1990). https://doi.org/10.1007/BF02319701

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  • DOI: https://doi.org/10.1007/BF02319701

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