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Gentamicin C: Separation of C1, C1a, C2, C2a and C2b components by HPLC using lsocratic ion-exchange chromatography and post-column derivatisation

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Summary

A HPLC system, using a strong cation exchanger and isocratic elution, was developed for the separation of the main, components of gentamicin (C1, C1a, C2, C2a) and C2b (sagamicin) in less than 20 minutes. The detection was performed by post-column derivatisation with o-phthalaldehyde and a fluorescence detector. The detection limit was 10ng for gentamicin C1. Some commercial gentamicin samples were analyzed.

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Author notes

  1. H. P. Nerad

    Present address: Biochemie Ges. m.b.H., Kundl, Austria

Authors and Affiliations

  1. Sandoz Forschungsinstitut, Wien, Austria

    G. Seidl & H. P. Nerad

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  1. G. Seidl
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  2. H. P. Nerad
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Seidl, G., Nerad, H.P. Gentamicin C: Separation of C1, C1a, C2, C2a and C2b components by HPLC using lsocratic ion-exchange chromatography and post-column derivatisation. Chromatographia 25, 169–171 (1988). https://doi.org/10.1007/BF02316439

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  • DOI: https://doi.org/10.1007/BF02316439

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