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Transgenic sweet potato plants obtained byAgrobacterium tumefaciens-mediated transformation

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Abstract

Stable expression of foreign genes was achieved in sweet potato (Ipomoea batatas (L.) Lam) plants using anAgrobacterium tumefaciens mediated system. Embryogenic calluses produced from apical meristems of cultivar White Star were multiplied and cocultivated withA. tumefaciens strain EHA101 harboring a binary vector containing the β-glucuronidase (GUS) and neomycin phosphotransferase (NPT II) genes. The calluses were transferred to selective regeneration medium and kanamycin resistant embryos were recovered which developed into morphologically normal plants. Histochemical and fluorimetric GUS assays of plants developed from the kanamycin resistant embryos were positive. Amplified DNA fragments were produced in polymerase chain reactions using GUS-specific primers and DNA from these plants. Transformation was confirmed by Southern analysis of the GUS gene. With the developed method, transgenic sweet potato plants were obtained within 7 weeks. This method will allow genetic improvement of this crop by the introduction of agronomically important genes.

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References

  • AllemAC (1987) Recursos genéticos em batata-doce. In: Seminario sobre a Cultura de Batata-doce (pp 37–54). Brasilia. EMBRAPA, CNPH

    Google Scholar 

  • BouwkampJC (1985) Sweet Potato Products: A Natural Resource For The Tropics. CRC Press Inc., Boca Raton, Florida (271 p.)

    Google Scholar 

  • BradfordMM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254

    Article  CAS  PubMed  Google Scholar 

  • Carelli ML, Skirvin RM & Harry DA (1991) Transformation and regeneration studies with “Jewel” sweetpotato. Sweetpotato Technology For The 21st Century (p 8), Tuskegee, Alabama

  • ChéeRP & CantliffeDJ (1988) Somatic embryos patterns and plant regeneration inIpomoea batatas Poir. In Vitro Cell Dev. Bio. 24: 955–958

    Google Scholar 

  • ChéeRP & CantliffeDJ (1989) Embryo development from discrete cell aggregates inIpomoea batatas (L.) Lam in response to structural polarity. In Vitro Cell Dev. Biol. 25: 757–760

    Google Scholar 

  • Gama MIC, Leite Jr. RP & Cantliffe DJ (1992) Transgenic sweet potato plants obtained byAgrobacterium tumefaciens mediated transformation. In: Biotechnology for Crop Improvement in Latin America (CTR-11), Caracas, Venezuela, 1992

  • GarvelNJ & JarretRL (1991) A modified CTAB DNA extraction procedure forMusa andIpomoea. Plant Mol. Biol. Rep. 9: 262–266

    Google Scholar 

  • HoodEE, HelmerGL, FraleyRT & ChiltonM-D (1986) Hypervirulence ofAgrobacterium tumefaciens A281 is encoded in a region of pTiBo 542 outside of T-DNA. J. Bacteriol. 168: 1291–1301

    CAS  PubMed  Google Scholar 

  • JarretRL, SalazarS & FernandezZR (1984) Somatic embryogenesis in sweet potato. Hortscience 19: 397–398

    Google Scholar 

  • JeffersonRA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387–405

    CAS  Google Scholar 

  • JoshiRL & JoshiV (1991) Strategies for expression of foreign genes in plants. FEBS 281: 1–8

    Article  CAS  Google Scholar 

  • KleeE & EstelleM (1991) Molecular genetic approach to plant hormone biology. Annu. Rev. Plant Physiol. 42: 529–551

    CAS  Google Scholar 

  • LangridgeWHR, FitzgeraldKH, KonczC, SchellJ & SzalayAA (1989) Dual promoter ofAgrobacterium tumefaciens mannopine synthase genes regulated by plant growth hormones. Proc. Natl. Acad. Sc. 86: 3219–3223

    CAS  Google Scholar 

  • LiuJR & CantliffeDJ (1984) Somatic embryogenesis and plant regeneration in tissue cultures of sweet potato (Ipomoea batatas Poir.). Pl. Cell Rep. 3: 112–115

    Google Scholar 

  • Lowe JM, Newel CA, Buitron F, Medina F & Dodds J (1992) Development of plant regeneration and transformation systems in sweet potato (Ipomoea batatas). World Congress On Cell and Tissue Culture (P-1121). 1992. Washington, Dc.

  • MooreGA, JacomoCC, NeidighJL, LawrenceSD & ClineK (1992)Agrobacterium-mediated transformation ofCitrus stem segments and regeneration of transgenic plants. Pl. Cell Rep. 11: 238–342

    CAS  Google Scholar 

  • MurashigeT & SkoogF (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol.Plant. 15: 473–497

    CAS  Google Scholar 

  • Nishiguchi M, Uehara Y & Komaki K (1992) Stable transformation of sweet potato by electroporation. In: World Congress On Cell And Tissue Culture (P-1143). 1992. Washington, DC

  • OtaniM, MiiM, HandaT, KamadaH & ShimadaT (1993) Transformation of sweet potato (Ipomoea batatas (L.) Lam.) plants byAgrobacterium rhizogenes. Plant Science 94: 151–159

    Article  CAS  Google Scholar 

  • PeirceLC (1987) Sweet Potato.In: Tuber and Tuberous Rooted Crop (pp 300–308). John Wiley, New York

    Google Scholar 

  • Porobo-Dessai A, Blay ET, Prakash CS & Nakamura K (1992) Expression of Gus A gene with an intron in sweet potato and garden egg plant. In: World Congress On Cell and Tissue Culture (P-1130), 1992. Washington DC

  • PrakashCS & VaradarajanU (1992) Genetic transformation of sweet potato by particle bombardment. Plant Cell Reports 11: 53–57

    Article  Google Scholar 

  • SaitoK, YamazakiM, KanekoMw, MurakoshiI, FukudaY & MontaguMC (1991) Tissue-specific and stress-enhancing expression of the TR promoter for mannopine synthase in transgenic medicinal plants. Planta 184: 41–46

    Article  Google Scholar 

  • SangerM, DaubertS & GoodmanM (1990) Characteristic of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter. Plant Mol. Biol. 14: 433–443

    Article  CAS  PubMed  Google Scholar 

  • ShahDM, TumerNE, FischhoffDA, HorschRB, RogersSG, FraleyRT & JaworskiEG (1987) The introduction and expression of foreign genes in plants. Biotechnology and Genetic Engineering Reviews 5: 82–107

    Google Scholar 

  • Templeton-SomersKM & CollinsWW (1986) Heritability of regeneration in tissue cultures of sweet potato (Ipomoea batatas Lam.). Theor. Appl. Genet. 781: 835–841

    Google Scholar 

  • TsayHS & TsengMT (1979) Embryoid formation and plantlet regeneration from anther callus of sweet potato. Bot. Bull. Acad. Sinica 20: 117–122

    Google Scholar 

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Authors and Affiliations

  1. CENARGEN/EMBRAPA, C. P. 02372, 70849-970, Brasilia, DF, Brazil

    Maria I. C. S. Gama

  2. IAPAR, Paraná, Brazil

    Rui P. Leite Jr.

  3. Departamento de Genética, UFRJ, Rio de Janeiro, Brazil

    Antonio R. Cordeiro

  4. Horticultural Sciences Department, University of Florida, Gainesville, USA

    Daniel J. Cantliffe

Authors
  1. Maria I. C. S. Gama
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  2. Rui P. Leite Jr.
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  3. Antonio R. Cordeiro
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  4. Daniel J. Cantliffe
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Additional information

Florida Agricultural Experiment Station Journal Series N-02231. This research was partially supported by CNPq/RHAE (Brazil).

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Gama, M.I.C.S., Leite, R.P., Cordeiro, A.R. et al. Transgenic sweet potato plants obtained byAgrobacterium tumefaciens-mediated transformation. Plant Cell Tiss Organ Cult 46, 237–244 (1996). https://doi.org/10.1007/BF02307100

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  • DOI: https://doi.org/10.1007/BF02307100

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