Abstract
The nodulation ability of variousFrankia strains isolated from the nodules of Casuarina were tested on two Casuarina species (C. equisetifolia andC. glauca), and onHippophaë rhamnoides. We found that the isolates could be separated into two groups, some of them being unable to reinfect the Casuarina host-plant but infective onH. rhamnoides. Other isolates effectively nodulated the original Casuarina host-plant. The second purpose of this study was to examine the genetic diversity among the Casuarina-isolated strains using well-characterized symbiotic genes as hybridization probes. We found a relationship between nodulation characteristics and hybridization patterns.
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References
An S C, Risggby W S and Mullin B C 1985 Relationships ofFrankia based on deoxyribonucleic acid homology studies. Int. J. Syst. Bacteriol. 35, 140–146.
Baker D 1987 Relationships among pure cultured strains ofFrankia based on host specificity. Physiol. Plant. 70, 245–248.
Baker D and O'Keefe D 1984 A modified sucrose fractionation procedure for the isolation of frankiae from actinorhizal root nodules and soil samples. Plant and Soil 78, 23–28.
Benson D R, Buchholz S E and Hanna D G 1984 Identification ofFrankia strains by two-dimensional polyacrylamide gel electrophoresis. Appl. Environ. Microbiol. 47, 489–494.
Birnboim H C and Doly J 1979 A rapid alkaline extraction procedure for screening recombinatn plasmid DNA. Nucleic Acids Res. 7, 1513–1523.
Callaham D, Torrey J G and Del Tredici P 1978 Isolation andin vitro characterization of an actinomycetous endophyte from Comptonia peregrina. Science 199, 899–902.
Caugant D A, Mocca L F, Frasch C E Frøholm L O,Zollinger W D and Selander R K 1987 Genetic structure ofNeisseria meningitidis populations in relation to serogroup, serotype and outer membrane protein pattern. J. Bacteriol. 169, 2781–2792.
Diem H G, Gauthier D and Dommergues Y 1982 Isolement et culturein vitro d'une souche infective et effective deFrankia isolée de nodules de Casuarina sp. C.R. Acad. Sc. Paris III 295, 759–763.
Diem H G and Dommergues Y 1983 The isolation ofFrankia from nodules of Casuarina. Can. J. Bot. 61, 2822–2825.
Dommergues Y R, Diem H G, Gauthier D L, Dreyfus B L and Cornet F 1984 Nitrogen-fixing trees in the tropics: Potentialities and limitations.In Advances in Nitrogen Fixation Research. Eds. C Veeger and W E Newton, pp 7–13. M Nijhoff/W Junk Nijhoff Publishers, The Hague.
Gardes M, Bousquet J and Lalonde M 1987 Isozyme variation among 40 Frankia strains. Appl. Environ. Microbiol. 53, 1596–1603.
Gauthier D, Diem H G and Dommergues Y 1981 Infectivité et effectivité des souches deFrankia isolées de nodules deCasuarina equisetifolia etHippophaë rhamnoides. C.R. Acad. Sc. Paris 293, 489–491.
Holmes D S and Quigley M 1981 A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114, 551–559.
Lalonde M 1979 Techniques and observations of the nitrogen-fixing Alnus root nodule symbiosis.In Recent Advances in Biological Nitrogen Fixation. Ed. N S Subba Rao, pp 421–434. Oxford and IBH Publication, New Delhi.
Lalonde M, Simon L, Bousquet J and Séguin A 1988 Advances in the taxonomy ofFrankia: recognition of speciesalni andelaeagni and novel subspeciespommerii andvandijkii.In Progress in Nitrogen Fixation. Eds. H Bothe and W E Newton. Gustav Fischer Verlag, Stuttgart. (In press).
Maniatis T, Fritsch E F and Sambrook J 1982 Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p 545.
Meusel H, Jäger E and Weinert E 1965 Vergleichende Chrologie der zentraleuropäischen Flora. VEB Gustav Fischer Verlag, Jena.
Miller J M 1972 Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Murray N E, Brammer W J and Murray K 1977 Lambdoid phages that simplify the recovery ofin vitro recombinants. Mol. Gen. Genet. 150, 53–61.
Murry M A, Fontaine M S and Torrey J G 1984 Growth kinetics and nitrogenase induction inFrankia sp. HFPArI3 grown in batch culture. Plant and Soil 78, 61–78.
Musser J M, Bemis D A, Ishikawa H and Selander R K 1987 Clonal diversity and host distribution inBordetella bronchiseptica. J. Bacteriol. 169, 2793–2803.
Normand P, Simonet P and Bardin R 1988 Conservation ofnif sequences inFrankia. Molecular and General Genetics 213, 238–246.
Puppo A, Dimitrijevic L, Diem H G and Dommergues Y R 1985 Homogeneity of superoxide dismutase patterns inFrankia strains from Casuarinaceae. FEMS Microbol. Letters 30, 43–46.
Reddell P and Bowen G D 1986 HostFrankia specificity within the Casuarinaceae. Plant and Soil 93, 293–298.
Rigby P W J, Dieckman M, Rhodes D and Berg P 1977 Labelling deoxyribonucleic acid to high specific activityin vitro by nick-translation with DNA polymerase. J. Mol. Biol. 113, 237–251.
Saint-Laurent L, Bousquet J, Simon L and Lalonde M 1988 Separation of variousFrankia strains in the Alnus and Elaeagnus host specificity groups using sugar analysis. Can. J. Microbiol. 33, 764–772.
Schildkraut C and Lifson S 1965 Dependence of the melting temperature of DNA on salt concentration. Biopolymers 3, 195–208.
Schwinghamer E A 1980 A method for improved lysis of Gram negative bacteria. FEMS. Microbiol Letters 7, 157–162.
Simonet P, Normand P, Moiroud A and Lalonde M 1985 Restriction enzyme digestion pattern ofFrankia plasmids. Plant and Soil 87, 49–59.
Simonet P, Haurat J, Normand P, Bardin R and Moiroud A 1986 Localization ofnif genes on a large plasmid inFrankia sp. strain ULQ0132105009. Mol. Gen. Genet. 204, 492–495.
Simonet P, Normand P, Hirsch A and Akkermans A D L 1989 The genetics of theFrankia actinorhizal symbiosis.In The Molecular Biology of Symbiotic Nitrogen Fixation, Ed. P M Gresshoff, CRC Press, Inc.In press.
Simonet P, Normand P and Bardin R 1989 Heterologous hybridization ofFrankia DNA toRhizobium meliloti andKlebsiella pneumoniae nif genes. FEMS Microbiol. Lett. 141–146.
Southern E M 1975 Detection of specific sequences among DNA fragment separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.
Takhtajan A 1969 Flowering Plants, Origin and Dispersal. Smithsonian Institution press, Washington, DC. 310 p.
Tautz D and Renz M 1983 An optimized freeze-sqeeze method for the recovery of DNA fragments from agarose gels. Anal. Biochem. 132, 14–19.
Thomas B A and Spicer R A 1987 The Evolution and Palaeobiology of Land Plants. Croom Helm, London. 309 p. ISBN 0-7099-2434-8.
Torrey J G 1978 Nitrogen fixation by actinomycete nodulated angiosperms. Bioscience 28, 586–591.
Zhang Z, Lopez M F and Torrey J G 1984 A comparison of cultural characteristics and infectivity ofFrankia isolates from root nodules of Casuarina species. Plant and Soil 78, 79–90.
Zhang Z and Torrey J G 1985 Studies of an effective strain ofFrankia fromAllocasuarina lehmaniana of the Casuarinaceae. Plant and Soil 87, 1–16.