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Immunological regression of metastatic cancer in the liver as a result of “in vivo xenogenization”

  • Published:
Biotherapy

Abstract

We attempted to induce the regression of liver metastatic tumor cellsin vivo by the administration to rats of Friend leukemia virus (FV) (in vivo xenogenization). The virus which was used in this experiment, FV, is highly immunogenic and does not normally cause disease in an adult rat. At first, we induced a FV viremia in tumor bearing rats in order to deliver the virus to the site of the tumor cells. FV viremia was induced by injecting 60 mg/kg cyclophosphamide (CY) i.v. after the administration of FV, and by transferring syngeneic bone marrow cells so that FV would be able to infect them and then replicate.

In order that the tumor cells which were infected with virus should regress, it was necessary to break down their tolerance to FV antigens. As adoptive immunotherapy we therefore, transferred syngeneic spleen cells from rats which had been immunized with FV to tumor bearing rats. The result of this experiment was that these tumor bearing rats infected with FV which had received either normal syngeneic spleen cells or no spleen cells as controls died from liver metastasis (8 out of 9 rats (89%) and 15 out of 17 (88%) respectively). On the other hand, only 4 out of the 15 (27%) tumor bearing rats which were infected with FV and which received FV-immune spleen cells died from liver metastasis.

These sets of data indicate that thein vivo xenogenization of tumor cells are indeed able to induce the regression of metastic tumor cells.

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Abbreviations

CY:

cyclophosphamide

FV:

Friend leukemia virus

MuLV:

murine leukemia virus

TAA:

tumor associated antigen

VAA:

virus associated antigen

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Authors and Affiliations

  1. Laboratory of Pathology, Hokkaido University School of Medicine, 060, Sapporo, Japan

    Yasuhiro Suzuki, Kiyoshi Morikawa, Jun-ichi Hamada, Michio Sugawara, Masuo Hosokawa & Hiroshi Kobayashi

  2. Laboratory of Genetics, Hokkaido University School of Medicine, 060, Sapporo, Japan

    Takeshi Okayasu

  3. Cancer Institute and The Second Department of Surgery, Hokkaido University School of Medicine, 060, Sapporo, Japan

    Tsuneyuki Oikawa

Authors
  1. Yasuhiro Suzuki
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  2. Takeshi Okayasu
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  3. Kiyoshi Morikawa
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  4. Jun-ichi Hamada
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  5. Michio Sugawara
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  6. Tsuneyuki Oikawa
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  7. Masuo Hosokawa
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  8. Hiroshi Kobayashi
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Suzuki, Y., Okayasu, T., Morikawa, K. et al. Immunological regression of metastatic cancer in the liver as a result of “in vivo xenogenization”. Biotherapy 2, 41–49 (1990). https://doi.org/10.1007/BF02172075

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  • DOI: https://doi.org/10.1007/BF02172075

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