Summary
Evolution and divergence among, species within the genusLathyrus have involved an approximately fivefold increase in the amounts of nuclear DNA. Most species inLathyrus are diploids with the same chromosome number, 2n=14. Significant changes in the amounts of repetitive sequences have accounted for much of the evolutionary DNA variation between species. Seven diploidLathyrus species with a twofold variation in nuclear DNA amounts between them were investigated. Using higher derivative analysis of the thermal denaturation profiles of the reassociated repetitive DNA, the reiteration frequency and divergence of repetitive families were compared. Much variation in the reiteration frequency was observed within and between species. In species with larger 2C DNA amounts repetitive families had on average greater amounts of DNA. Despite the massive differences in DNA amounts, six species were consistently similar in the number of repetitive families in their genomes, and they showed a similar pattern in base sequence divergence. In terms of base sequence relationships the repetitive families appeared to be heterogeneous. The evolutionary significance is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bedbrook JR, O'Dell M, Flavell RB (1980) Amplification of rearranged sequences in cereal plants. Nature 288:133–137
Bendich AJ, Anderson RS (1977) Characterization of families of repeated DNA sequences from four vascular plants. Biochemistry 16:4655–4663
Britten RJ, Kohne DE (1968) Repeated sequences in DNA. Science 161:529–540
Britten RJ, Graham DE, Neufeld BR (1974) Analysis of repeating DNA sequences by reassociation. In: Grossman L, Moldave K (eds) Methods in enzymology, vol 29, part E. Nucleic acids and protein synthesis. Academic Press, New York, p 363
Butler WL, Hopkins DW (1970) Higher derivative analysis of complex absorption spectra. Phytochem Photobiol 12:439–450
Cueller RE, Ford GA, Briggs WR, Thompson WF (1978) Application of higher derivative techniques to analysis of, high resolution denaturation profiles of reassociated repeated DNA. Proc Natl Acad Sci USA 75:6026–6030
Dover GA (1980) Ignorant DNA. Nature (London) 285:618–620
Kimura M (1985) Natural selection and neutral evolution. In: Godfrey LR (ed) What Darwin began. Allyn and Bacon, Boston, p 73
Melchior WB, Von Hippel PH (1973) Alteration of the relative stability of of dA-dT and dG-dC base pairs in DNA. Proc Natl Acad Sci USA 70:298–302
Narayan RKJ (1982) Discontinuous DNA variation in the evolution of plant species. The genusLathyrus. Evolution 36: 877–891
Narayan RKJ (1985) Discontinuous DNA variation in the evolution of plant species. J Genet 64:101–109
Narayan RKJ, Rees H (1976) Nuclear DNA variation inLathyrus. Chromosoma (Berlin) 54:141–154
Preisler RS, Thompson WF (1981) Evolutionary sequence divergence within repeated DNA families of higher plant genomes. J Mol Evol 17:85–93
Raina SN, Narayan RKJ (1984) Changes in DNA composition in the evolution ofVicia species. Theor Appl Genet 68:187–192
Rees H, Narayan RKJ (1977) Evolutionary DNA variation inLathyrus. In: de la Chappelle A, Sorsa M (eds) Chromosomes today, vol 6. Elsevier/North Holland Biomedical Press, Amsterdam, pp 131–139
Southern EM (1974) Eukaryotic DNA. In: Biochemistry of nucleic acids. Biochemistry series 6. Butterworths, London, pp 101–141
Thompson WF, Murray MG (1980) Sequence organisation in pea and Mung bean DNA and a model for genome evolution. In: Davies DR, Hopwood DA (eds) The plant genome. John Innes Symposium. John Innes Charity, Norwich, England, pp 31–45
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kuriyan, P.N., Narayan, R.K.J. The distribution and divergence during evolution of families of repetitive DNA sequences inLathyrus species. J Mol Evol 27, 303–310 (1988). https://doi.org/10.1007/BF02101192
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02101192