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Identification ofChlamydia pneumoniae-specific protein antigens in immunoblots

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Abstract

The immunoblot patterns of 248 sera, all examined previously by the microimmunofluorescence test (MIF) for species-specificChlamydia antibodies, were analyzed. Predominant specific antibody activity was directed to the 54 kDa protein ofChlamydia pneumoniae, which was recognized by 93 % of sera positive forChlamydia pneumoniae by MIF but by only 2 % of sera positive forChlamydia trachomatis and negative forChlamydia pneumoniae and by 3 % of sera negative for bothChlamydia pneumoniae andChlamydia trachomatis. This antigen appears to be specific forChlamydia pneumoniae. OtherChlamydia pneumoniae-specific protein antigens were recognized far less frequently. Absorption analysis indicated that the 54 kDa protein is located on the surface of theChlamydia pneumoniae elementary bodies.

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Authors and Affiliations

  1. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herber-Str. 11, D-79104, Freiburg, Germany

    H. M. Freidank, A. S. Herr & E. Jacobs

Authors
  1. H. M. Freidank
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  2. A. S. Herr
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  3. E. Jacobs
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Freidank, H.M., Herr, A.S. & Jacobs, E. Identification ofChlamydia pneumoniae-specific protein antigens in immunoblots. Eur. J. Clin. Microbiol. Infect. Dis. 12, 947–951 (1993). https://doi.org/10.1007/BF01992171

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  • DOI: https://doi.org/10.1007/BF01992171

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