Summary
To evaluate the effects of pantothenic acid during wound healing processes, fibroblastic cell cultures originating from foreskin were established and subcultured by trypsinization. PA (40 µg/ml) was added to the basal culture medium. The cell proliferation was estimated by cell count and determination of3H-thymidine incorporation. The protein synthesis and secretion were determined by dosage in the cells and in the culture medium.
When PA was added to the medium, a significant increase of cell proliferation and of3H-thymidine incorporation was observed mainly during the first few days. PA also stimulated intracellular protein synthesis, but did not induce a release of proteins in the culture medium. The exact mechanism involved in this phenomenon remains unclear at this time.
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Lacroix, B., Didier, E. & Grenier, J.F. Effects of pantothenic acid on fibroblastic cell cultures. Res. Exp. Med. 188, 391–396 (1988). https://doi.org/10.1007/BF01851207
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DOI: https://doi.org/10.1007/BF01851207