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Effect of subarachnoid haemorrhage on trigeminovascular calcitonin-gene-related peptide and substance P of the rat dura mater versus cerebral vasculature

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Summary

While the presence of a robust perivascular neural network accompanying cerebral and dural blood vessels that contain various neuropeptides is well documented, the functional significance of this innervation is unclear.

Following experimentally induced subarachnoid haemorrhage (SAH) in animal models, immunocytochemical studies have revealed that changes occur in the staining intensity of some of these neuropeptides. This study compared the immunostaining intensity of calcitonin-gene-related peptide (CGRP) and substance P (SP) in cerebral and dural perivascular nerve fibers after SAH in the rat. Subarachnoid haemorrhage was produced by injecting 0.3 ml of autologous blood into the cisterna magna of male Sprague Dawley rats. Sham operated animals received an equal volume of buffered lactated Ringer's solution (pH 7.4). Changes in the immunostaining intensity of cerebral and dural vessels were evaluated by independent observers at 6, 24, and 48 hours after SAH. Immunostaining of CGRP was reduced in cerebral vessels at 6 hours and returned to normal by 48 hours. In contrast, CGRP immunostaining of dural perivascular nerve fibers was unchanged at all time periods examined. A marked decrease in SP immunostaining was documented at 6 hours in both the cerebral and dural vessels in all animals; at 48 hours, the staining intensity had returned to control levels.

These results support the idea that several subpopulations of trigeminovascular neurons containing CGRP, SP, or both project to cerebral and dural vessels. Since these subpopulations may be differentially activated in pathologic conditions, such as SAH or vascular headache, the potential exists for pharmacologic intervention of specific neuropeptides with the resultant abatement of a pathologic process.

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Authors and Affiliations

  1. Department of Neurosurgery, University of Cincinnati College of Medicine, Mayfield Neurological Institute, Cincinnati, OH, USA

    A. G. Arand, M. Zuccarello, B. A. Skidmore, R. J. Berger & J. T. Keller

  2. The Christ Hospital, James N. Gamble Institute of Medical Research, Cincinnati, OH, USA

    A. G. Arand, M. Zuccarello, B. A. Skidmore, R. J. Berger & J. T. Keller

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  1. A. G. Arand
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  2. M. Zuccarello
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  3. B. A. Skidmore
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This work was supported in part by grants from NIH NS 22969 (JTK) and the Elizabeth Gamble Deaconess Home Association (JTK).

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Arand, A.G., Zuccarello, M., Skidmore, B.A. et al. Effect of subarachnoid haemorrhage on trigeminovascular calcitonin-gene-related peptide and substance P of the rat dura mater versus cerebral vasculature. Acta neurochir 127, 103–111 (1994). https://doi.org/10.1007/BF01808556

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