Summary
Phylogenetic relationships ofOsmunda cinnamomea, O. claytoniana, andO. regalis were explored by means of DNA sequence comparisons. Hydroxyapatite thermal elution profiles of self-reassociated repetitive DNA fragments were very similar, indicating the absence of gross differences in the amount of recent amplification or addition of repetitive DNA in any of these three genomes. Interspecific DNA sequence comparisons showed, in contrast to our earlier interpretation, that repeated DNA sequences ofO. claytoniana are nearly equally diverged from those ofO. cinnamomea andO. regalis. Differences between repetitive sequences of the three species can be interpreted as reflecting amplification events which occurred subsequent to speciation. The data obtained suggest that the threeOsmunda species most likely arose more or less simultaneously from a common ancestor. These findings were verified in experiments with tracer DNA preparations enriched for single copy sequences. On the basis of the hybridization data presented here and of the fossil record, the rate of single copy sequence divergence in the ferns is comparable to that in the primates, although slower than that observed in other animal taxa. From this first evaluation of rates of DNA evolution in plants it would seem that the rates for plants and animals are roughly comparable. The evidence suggests that species divergence is accompanied by further reiteration of preexisting repeat sequences. The rate of addition of repetitive sequences probably is slower in ferns than in angiosperms. This difference might be attributable to the much larger effective generation time in ferns.
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References
Angerer, R.C., Davidson, E.H., Britten, R.J. (1976). Single copy DNA and structural gene sequence relationships among four sea urchin species. Chromosoma56, 213–226
Arnold, C.A. (1964). Mesozoic and Tertiary fern evolution and distribution. Mem. Torrey Bot. Club21, 58–66
Bendich, A.J., Anderson, R.S. (1977). Characterization of repeated DNA sequences from four vascular plants. Biochemistry16, 4655–4663
Bendich, A.J., McCarthy, B.J. (1970). DNA comparisons among barley, oats, rye and wheat. Genetics65, 545–565
Bierhorst, D.W. (1971). Morphology of vascular plants. New York: Macmillan
Bobrov, A.E. (1967). The family Osmundaceae (R.Br.) Kaulf. Its taxonomy and geography. (In Russian). Bot. Zhurn.52, 1600–1610
Bonner, T.I., Brenner, D.J., Neufeld, B.R., Britten, R.J. (1973). Reduction in the rate of DNA reassociation by sequence divergence. J. Mol. Biol.81, 122–135
Bouchard, R.A., Swift, H. (1977). Nature of the heterogeneity in mispairing of reannealed middle-repetitive fern DNA. Chromosoma61, 317–333
Britten, R.J., Graham, D.E., Neufeld, B.R. (1974). Analysis of repeating DNA sequences by reassociation. In: Methods in Enzymology. XXIX (L. Grossman and K. Moldave, eds.) New York: Academic Press
Britten R.J., Kohne, D.E. (1968). Repeated sequences in DNA. Science161, 529–540
Copeland, E.B. (1947). Genera filicum. New York: Ronald Press
Commerford, S.L. (1971). Iodination of nucleic acids in vitro. Biochemistry10, 1993–1999
Diels, L. (1902). Die natürlichen Pflanzenfamilien.1.4, 372–380
Faull, J.H. (1901). The anatomy of the Osmundaceae. Bot. Gaz.31, 381–420
Flavell, R.B., Rimpau, J., Smith, D.B. (1977). Repeated sequence DNA relationships in four cereal genomes. Chromosoma63, 205–222
Galau, G.A., Chamberlin, M.E., Hough, B.R., Britten, R.J., Davidson, E.H. (1976). Evolution of repetitive and nonrepetitive DNA. In: Molecular Evolution (F.J. Ayala, ed.) Sunderland, Ma: Sinauer Associates
Harpold, M.H., Craig, S.P. (1978). The evolution of nonrepetitive DNA in sea urchins. Differentiation10, 7–11
Hewitson, W. (1962). Comparative morphology of the Osmundaceae. Ann. Missouri Bot. Gard.49, 57–93
Kohne, D.E. (1970) Evolution of higher organism DNA. Quart. Rev. Biophysics33, 327–375
Laird, C., McConaughy, B.L., McCarthy, B.J. (1969). Rate of fixation of nucleotide substitution in evolution. Nature, London224, 149–154
McDonnell, M.W., Simon, M.N., Studier, E.W. (1977). Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J. Mol. Biol.110, 119–146
Miller, C.N. (1967). Evolution of the fern genusOsmunda. Contrib. Mus. Paleontol. Univ. Mich.21, 139–203
Miller, C.N. (1971). Evolution in the fern family Osmundaceae based on anatomical studies. Contrib. Mus. Paleontol. Univ. Mich.23, 105–169
Mizuno, S., Macgregor, H.C. (1974). Chromosomes, DNA sequences and evolution in salamanders of the genusPletbodon. Chromosoma (Berl.)48, 239–296
Muller, J. (1970). Palynological evidence on early differentiation of angiosperms. Biol. Rev.45, 417–450
Murray, M.G., Belford, H.S., Thompson, W.F. (1977). In vitro labeling of singlestranded DNA. Carnegie Inst. Wash. Year Book76, 262–267
Murray, M.G., Thompson, W.F. (1977). Single-strand DNA fragment length determination by alkaline agarose electrophoresis: applications for DNA renaturation studies. Carnegie Inst. Wash. Year Book76, 259–262
Narayan, R.K.J., Rees, H. (1977). Nuclear DNA divergence amongLathyrus species. Chromosoma63, 101–107
Orosz, J.M., Wetmur, J.G. (1974). In vitro iodination of DNA. Maximizing iodination while minimizing degradation; use of buoyant density shifts for DNA-DNA hybrid isolation. Biochemistry13, 5467–5473
Pearson, W.R., Davidson, E.H., Britten, R.J. (1977). A program for least squares analysis of reassociation and hybridization data. Nucleic Acids Research4, 1727–1735
Petersen, R.L., Fairbrothers, D.E. (1971). North AmericanOsmunda species: a serologic and disc electrophoretic analysis of spore proteins. Am. Midl. Nat.85, 437–457
Prensky, W., Steffensen, D.M., Hughes, W.L. (1973). The use of iodinated RNA for gene localization. Proc. Nat. Acad. Sci. USA70, 1860–1864
Presl, C.B. (1847). Supplementum Tentaminis Pteridographiae. Abh. Böhm. Ges. Wiss. V. 4, 261–380
Rice, N. (1972). Changes in repeated DNA in evolution. In: Evolution of Genetic Systems. Brookhaven Symp. Biol.23, 44–78
Smith, D.B., Flavell, R.B. (1974). The relatedness and evolution of repeated nucleotide sequences in the genomes of some Gramineae species. Biochem. Genet.12, 243–256
Sparrow, A.H., Price, H.J., Underbrink, A.G. (1972). A survey of DNA content per cell and per chromosome of prokaryotic and eukaryotic organisms: some evolutionary considerations. In: Evolution of Genetic Systems. Brookhaven Symp. Biol.23, 451–494
Stebbins, G.L. Cytogenetics and evolution of the grass family. Amer. Jour. Bot.43, 890–905
Stein, D.B., Thompson, W.F. (1975). DNA hybridization and evolutionary relationships in threeOsmunda species. Science189, 888–890
Stein, D.B., Thompson, W.F. (1977). Interspecific hybridization of fern DNA. Carnegie Inst. Wash. Year Book76, 252–255
Stein, D.B., Thompson, W.F. (1978). Isolation of DNA from tannin-containing plants. Plant Sci. Letters11, 323–328
Studier, F.W. (1965). Sedimentation studies of the size and shape of DNA. J. Mol. Biol.11, 373–390
Tagawa, M. (1941). Osmundaceae of Formosa. J. Jap. Bot.17, 692–703
Vaughn, J.C., Traeger, F.J. (1976). Conservation of repeated DNA base sequences in the Crustaceae: a molecular approach to decapod phylogeny. J. Mol. Evol.7, 111–131
Wagner, W.H., Wagner, F.S., Miller, C.N., Wagner, D.H. (1978). New observations on the royal fern hybridOsmunda × ruggii. Rhodora80, 92–106
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Stein, D.B., Thompson, W.F. & Belford, H.S. Studies on DNA sequences in the Osmundaceae. J Mol Evol 13, 215–232 (1979). https://doi.org/10.1007/BF01739481
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DOI: https://doi.org/10.1007/BF01739481