Skip to main content
SpringerLink
Log in

Evolutionary sequence divergence within repeated DNA families of higher plant genomes

II. Analysis of Thermal Denaturation

  • Published:
Journal of Molecular Evolution Aims and scope Submit manuscript

Abstract

An assay based on derivative analysis of thermal denaturation (melting) behavior of reassociated DNA was developed in an attempt to characterize the sequence relationships in repeated DNA families according to the homogeneous or heterogeneous models of Bendich and Anderson (1977). The validity of the technique was confirmed by the use of deaminatedEscherichia coli DNA models for repetitive families. The melting data for DNA reassociated at two different temperatures provided strong evidence thatPisum sativum repeated families are mostly heterogeneous, while homogeneous families predominate inVigna radiata. These findings, together with other differences between the two genomes, suggest that the rate of sequence amplification has been higher in the evolutionary history ofPisum DNA. A general trend seems to exist for high amplification rates in large, highly repetitive plant genomes such asPisum and lower rates in smaller plant genomes such asVigna, as well as in the generally smaller, less repetitive genomes of most animal species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

(Et)4NCl:

tetraethylammonium chloride

C0t:

the product of molar concentration of DNA nucleotides and time of incubation (mol s/L)

PIPES:

1,4-piperazinedietha-nesulfonic acid

Tm :

the temperature at which half of the nucleotides in solution are unpaired

SSC:

150 mM NaCl-15 mM sodium citrate

HAP:

hydroxylapatite

NTP:

nucleotide pairs

References

  • Bedbrook JR, Jones J, O'Dell M, Thompson RD, Flavell RB (1980) Cell 19:545–560

    Google Scholar 

  • Bendich AJ, Anderson RS (1977) Biochem 16:4655–4663

    Google Scholar 

  • Bennett MD, Smith JB (1976) Philos Trans R Soc London Ser B 274:227–273

    Google Scholar 

  • Bouchard RA, Swift H (1977) Chomosoma 61:317–333

    Google Scholar 

  • Britten RJ, Graham DE, Neufeld BR(1974) In: Grossman L, Moldave K (eds) Methods in Enzymology 29, Part E. Nucleic Acids and Protein Synthesis. Academic Press, New York, p 363

    Google Scholar 

  • Britten RJ, Kohne DE (1968) Science 161:529–540

    Google Scholar 

  • Cuellar RE, Ford GA, Briggs WR, Thompson WF (1978) Proc Natl Acad Sci USA 75:6026–6030

    Google Scholar 

  • Flavell RB, Rimpau J, Smith DB (1977) Chromosoma 63:205–222

    Google Scholar 

  • Flavell RB, Bedbrook J, Jones J, O'Dell M, Gerlach WL, Dyer TA, Thompson RD (1980) In: Davies DR, Hopwood DA (eds) The Plant Genome. John Innes Charity, Norwich, UK, p 15

    Google Scholar 

  • Hinegardner R (1976) In: Ayala FJ (ed) Molecular Evolution. Sinauer Associates, Sunderland, p 179

    Google Scholar 

  • Marsh JL, McCarthy BJ (1974) Biochem 13:3382–3388

    Google Scholar 

  • Martinson HG, Wagenaar EB (1974) Anal Biochem 61:144–154

    Google Scholar 

  • Martinson HG, Wagenaar EB (1977) Biochim Biophys Acta 474:445–455

    Google Scholar 

  • Mizuno S, Andrews C, MacGregor HC (1976) Chromosoma 58:1–31

    Google Scholar 

  • Moore GP, Scheller RH, Davidson EH, Britten RJ (1978) Cell 15:649–660

    Google Scholar 

  • Murray MG, Cuellar RE, Thompson WF (1978) Biochem 17:5781–5790

    Google Scholar 

  • Murray MG, Palmer JD, Cuellar RE, Thompson WF (1979) Biochem 18:5259–5266

    Google Scholar 

  • Murray MG, Peters DA, Thompson WF (1980) J Mol Evol (in press)

  • Preisler RS, Thompson WF (1980) J Mol Evol

  • Rimpau J, Smith DB, Flavell RB (1978) J Mol Biol 123:327–359

    Google Scholar 

  • Smith MJ, Britten RJ, Davidson EH (1975) Proc Natl Acad Sci USA 72:4805–4809

    Google Scholar 

  • Thompson WF (1978) Carnegie Inst Washington Yearb 77:310–316

    Google Scholar 

  • Thompson EF, Murray MG (1980a) In: Davies DR, Hopwood DA (eds) The Plant Genome. John Innes Charity, Norwich, UK, p 31

    Google Scholar 

  • Thompson WF, Murray MG (1980b) In: Marcus A (ed) The Biochemistry of Plants: A Comprehensive Treatise VI. Academic Press, New York (in press)

    Google Scholar 

  • Ullman JS, McCarthy BJ (1973) Biochim Biophys Acta 294:416–424

    Google Scholar 

  • Wetmur JG (1976) Ann Rev Biophys Bioeng 5:337–361

    Google Scholar 

Download references

Author information

Author notes

  1. Richard S. Preisler

    Present address: Department of Biology, University of Pennsylvania, 19104, Philadephia, PA, USA

Authors and Affiliations

  1. Department of Plant Biology, Carnegie Institution of Washington, Stanford University, 94305, Stanford, California, USA

    Richard S. Preisler & William F. Thompson

  2. Department of Biological Sciences, Stanford University, 94305, Stanford, California, USA

    Richard S. Preisler & William F. Thompson

Authors
  1. Richard S. Preisler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William F. Thompson
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This paper is Carnegie Institution of Washington Publication No. 709 and is based on a portion of a dissertation submitted by R.S.P. in partial fulfillment of the Ph. D. requirements at Stanford University

Rights and permissions

Reprints and permissions

About this article

Cite this article

Preisler, R.S., Thompson, W.F. Evolutionary sequence divergence within repeated DNA families of higher plant genomes. J Mol Evol 17, 85–93 (1981). https://doi.org/10.1007/BF01732678

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01732678

Key words

Search

Navigation