Abstract
Large amounts of pathogenesis-related (PR) proteins were found inNicotiana tabacum crown gall tissue, following transformation of normal tobacco cells withAgrobacterium tumefaciens. In contrast, PR proteins were not detected in leaves of grafted plants that had been recovered from crown gall tissue even though these plants were still transformed as shown by their inability to form roots and ability to produce octopine. No difference was observed in susceptibility to virus infection between untransformed and transformed plants grafted onto identical rootstocks. The results are discussed in relation to physiological factors controlling PR protein induction and virus resistance.
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Ahl P, Benjana A, Samsom R, Gianizazzi S: Induction chez le tabac par Pseudomonas syringae nouvelles protéines (protéines ‘b’) associées au développement d'une résistance non spécifique à une deuxième infection. Phytopath Z 102: 201–212, 1981.
Akiyoshi DE, Morris RO, Hinz R, Mischke BS, Kosuge T, Garfinkel DJ, Gordon MP, Nester EW: Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. Proc Natl Acad Sci USA 80:407–411, 1983.
Antoniw JF, Pierpoint WS: The purification and properties of one of the b′ proteins from virus-infected tobacco plants. J Gen Virol 39:343–350, 1978.
Antoniw JF, White RF: The effects of aspirin and polyacrylic acid on soluble leaf proteins and resistance to virus infection in five cultivars of tobacco. Phytophath Z 98:331–341, 1980.
Antoniw JF, Ritter CE, Pierpoint WS, Van Loon LC: Comparison of three pathogenesis-related proteins from plants of two cultivars of tobacco infected with TMV. J Gen Virol 47:79–87, 1980.
Antoniw JF, Kueh JSH, Walkey DGA, White RF. The presence of pathogenesis related proteins in callus of Xanthine tobacco. Phytopath Z 101:179–184, 1981.
Chilton MD, Drummond MH, Merlo DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW: Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11:263–271, 1977.
Davis BJ: Disc electrophoresis II Method and application to human serum proteins. Ann NY Acad Sci 121:404–427, 1964.
Drummond MH, Gordon MP, Nester EW, Chilton MD: Foreign DNA of bacterial plasmid origin transcribed in crown gall tumors. Nature 269:535–536, 1977.
Gianinazzi S, Martin C, Vallee JC: Hypersensibilité aux virus, température et protéines solubles chez le Nicotiana Xanthti nc. Apparition de nouvelles macromolécules lors de la répression de la synthèse virale. Cr Acad Sci Paris D 270:2382–2386, 1970.
Gianinazzi S, Kassanis B: Virus resistance induced in plants by polyacrylic acid. J Gen Virol 23:1–9, 1974.
Gianinazzi S, Ahl P, Cornu A, Scalla R: First report of host b-protein appearance in response to a fungal infection in tobacco. Physiol Plant Pathol 16:337–342, 1980.
Murashige T, Skoog F: A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol Plantarum 15:473–497, 1962.
Ornstein L: Disc electrophoresis I Background and theory. Ann NY Acad Sci 121:21–349, 1964.
Van Loon LC: Polyacrylamide disc electrophoresis of the soluble leaf protein from Nicotiana tabacum var ‘Samsun’ and ‘Samsun NN’. IV Similarity of qualitative changes of specific proteins after infection with different viruses and their relationship to acquired resistance. Virology 67:566–575, 1975.
Van Loon LC: Induction by 2-chloroethylphosphonic acid of viral-like lesions, associated proteins, and systemic resistance in tobacco. Virology 80:417–420, 1977.
Van Loon LC, Van Kammen A: Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var ‘Samsun’ and ‘Samsun NN’. II Changes in protein constitution after infection with tobacco mosaic virus. Virology 40:199–211, 1970.
White RF: Acetyl salicylic acid (aspirin) induces resistance to TMV in tobacco. Virology 99:410–412, 1979.
Willmitzer L, De Beuckeleer M, Lemmers M, Van Montagu M, Schell J: DNA from Ti plasmid present in nucleus and absent from plastids of crown gall plant cells. Nature 287:359–361, 1980.
Wullems GJ, Molendijk L, Ooms G, Schilperoort RA: Retention of tumor markers in F1 progeny plants from in vitro induced octopine and nopaline tumor tissues. Cell 24: 719–727, 1981.
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Antoniw, J.F., Ooms, G., White, R.F. et al. Pathogenesis-related proteins in plants and tissues ofNicotiana tabacum transformed byAgrobacterium tumefaciens . Plant Mol Biol 2, 317–320 (1983). https://doi.org/10.1007/BF01578593
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DOI: https://doi.org/10.1007/BF01578593