Abstract
Two procedures were used to isolateAnaplasma marginale bodies from bovine erythrocytes. DNA extracted from bodies prepared by the second method was free of any detectable bovine DNA contamination.Anaplasma marginale DNA was analyzed by agarose gel electrophoresis of endonuclease restriction fragments and by reassociation kinetics. Genome size was estimated to be 340 kb. Base composition of the DNA was 33 mol% guanine+ cytosine (G+C), determined from its thermal denaturation temperature.Anaplasma marginale has a very small genome compared with that of other bacteria and has a low G+C content. It is proposed thatA. marginale may be a close, but degenerate, relative of the rickettsiae.
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Bear, P.D., Philpott, M.S. Isolation and characterization ofAnaplasma marginale DNA. Current Microbiology 15, 171–175 (1987). https://doi.org/10.1007/BF01577268
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DOI: https://doi.org/10.1007/BF01577268