Abstract
The production by T cells of an antigen-specific factor capable of replacing the T-cell function in specific antibody formation was used as a tool for studying the cellular aspects of the genetic control of immune responses. The ability of different T-cell populations to produce a cooperative signal and the ability of B-cell populations to react to this signal were studied in different mouse strains. The antigen used was the synthetic polypeptide poly(LTyr,LGlu)-poly-(LPro) —poly(lXys), (T,G)-Pro -L, the response to which was found not to beH-2-linked. It was found that the SWR strain of mice, a low responder to (T,G)-Pro -L, is not capable of producing a T-cell factor specific to this antigen, but its B cells react normally to an active factor produced in a high responder strain. In the DBA/1 strain, also a low responder to (T,G)-Pro -L, the bone marrow cells are not able to cooperate with an active T-cell factor to produce anti-(T,G)-Pro —L-specific antibodies, while their T cells do produce a (T,G)-Pro -L-specific factor. The SWR (low responder) B cells can be triggered by DBA/1 (low responder) T cells factor specific to (T,G)-Pro —L to produce an antibody response to this immunogen. These results suggest that the immune response to (T,G)-Pro -L is controlled by two genes which are expressed in different lymphocyte populations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Benacerraf, B. and McDevitt, H.O.: The histocompatibility-linked immune response genes.Science 175:273–279, 1972
Bonavida, B., Mozes, E., Shearer, G.M., and Sela, M.: Immunological unresponsiveness induced in adult mice to synthetic polypeptides built on multichain polyproline and multichain polyalanine.Immunochemistry 11:347–353, 1974
Fuchs, S. and Sela, M.: Antigenicity of some new synthetic polypeptides and polypeptidyl gelatins.Biochem. J. 93:566–572, 1964
Herbert, W.J.: Passive hemagglutination with special reference to the tanned cell technique.In D.M. Weir (ed.):Handbook of Experimental Immunology, 20.1–20.20, Blackwell, Oxford, 1973
Jaton, J.-C. and Sela, M.: Role of optical configuration in the immunogenicity and specificity of synthetic antigens derived from multichain polyproline.J. Biol. Chem. 243:5616–5626, 1968
Jerne, N.K. and Nordin, A.H.: Plaque formation in agar by single antibody-producing cells.Science 140:405, 1963
Mozes, E.: Expression of immune response (Ir) genes in T and B cells.Immunogenetics 2:397–410, 1975
Mozes, E.: The nature of antigen-specific T cell factors involved in the genetic regulation of immune responses.In D.H. Katz and B. Benacerraf (eds.):The Role of the Products of the Histocompatibility Gene Complex in Immune Responses, Academic Press, New York, 485–505, 1976
Mozes, E. and Isac, R.: Antigen-specific T cell factors in the genetic control of the immune response to poly(Tyr,Glu)-poly(Pro) —poly(Lys).In M. Feldman and A. Globerson (eds.):Immune Reactivity of Lymphocytes. Development, Expression and Control, Adv. Exp. Med. and Biology, Vol. 66, Plenum Publishing Co., New York, 547–549, 1976
Mozes, E., Isac, R., and Taussig, M.J.: Antigen-specific T cell factors in the genetic control of the immune response to poly(Tyr,Glu)-polyDL-Ala -poly Lys.J. Exp. Med. 141:703–707, 1975
Mozes, E., McDevitt, H.O., Jaton, J.-C, and Sela, M.: The nature of the antigenic determinant in a genetic control of the antibody response.J. Exp. Med. 150:493–504, 1969 a
Mozes, E., McDevitt, H.O., Jaton, J.-C, and Sela, M.: The genetic control of antibody specificity.J. Exp. Med. 150:1263–1278, 1969b
Mozes, E. and Sela, M.: The role of the thymus in a genetically controlled defect of the immune response at the carrier level.Proc. Nat. Acad. Sci. (USA) 71:1574–1577, 1974
Mozes, E. and Shearer, G.M.: Genetic control of immune responses.Curr. Top. Microbiol. Immunol. 59:167–200, 1972
Munro, A.J. and Taussig, M.J.: Two genes in the major histocompatibility complex control immune responses.Nature 256:103–106, 1975
Munro, J., Taussig, M.J., Campbell, R., Williams, H., and Lawson, Y.: Antigen-specific T cell factor in cell cooperation: physical properties and mapping in the left-hand (K) half of H-2.J. Exp. Med. 140:1579–1587, 1974
Shearer, G.M., Mozes, E., and Sela, M.: Contribution of different cell types to the genetic control of immune responses as a function of the chemical nature of the polymeric side chains (polyLPro and polyDLAla) of synthetic immunogens.J. Exp. Med. 135:1009–1027, 1972
Taussig, M.J.: T cell factor which can replace the activity of T cellsin vivo.Nature (Lond.) 248:234–236, 1974
Taussig, M.J., Mozes, E., and Isac, R.: Antigen-specific thymus cell factors in the genetic control of the immune response to (T,G)-A-L.J. Exp. Med. 140:301–313, 1974
Taussig, M.J., Munro, A.J., Campbell, R., David, C.S., and Staines, N.A.: Antigen-specific T cell factor in cell cooperation. Mapping within the I region of the H-2 complex and ability to cooperate across allogeneic barriers.J. Exp. Med. 142:694–700, 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Isac, R., Mozes, E. & Taussig, M. Antigen-Specific T-cell factors in the immune response to poly(Tyr,Glu)-poly(Pro)-poly(Lys). Immunogenetics 3, 409–416 (1976). https://doi.org/10.1007/BF01576972
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01576972