Abstract
We previously observed that whenAzotobacter vinelandii was transformed by different broad-host-range plasmids, normal cellular functions such as growth and siderophore production are impaired. In the present work, whenA. vinelandii was transformed with the low copy number plasmid pRK290, the extent of this metabolic impairment was lessened, as evidenced by increased siderophore production and moderate levels of growth on medium that lacks added iron. It is concluded that the severity of the plasmid-induced metabolic load reflects the relative level of expression of plasmid-encoded proteins.
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Literature Cited
Baldani VLD, Baldani JI, Dobereiner J (1983) Effects ofAzospirillum inoculation on root infection and nitrogen incorporation in wheat. Can J Microbiol 29:924–929
Barth PT, Tobin L, Sharpe GS (1981) Development of broad-host-range plasmid vectors. In: Levy SB, Clowes RC, Koenig EL (eds) Molecular biology, pathogenicity, and ecology of bacterial plasmids. New York: Plenum Press, pp 439–448
Bashan Y (1986) Enhancement of wheat root colonization and plant development byAzospirillum brasilense Cd following temporary depression of rhizosphere microflora. Appl Environ Microbiol 51:1067–1071
Brown ME (1982) Nitrogen fixation by free-living bacteria associated with plants—fact or fiction.In: Rhodes-Roberts ME, Skinner FA (eds) Bacteria and plants. London: Academic Press, pp 26–41
Ditta, G, Stanfield S, Corbin D, Helinski DR (1980) Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank ofRhizobium meliloti. Proc Natl Acad Sci USA 77:7347–7351
Glick BR, Whitney GK (1987) Factors affecting the expression of foreign proteins inEscherichia coli. J Ind Microbiol 1:277–282
Glick BR, Brooks HE, Pasternak JJ (1985) Transformation ofAzotobacter vinelandii with plasmid DNA. J Bacteriol 162:276–279
Glick BR, Brooks HE, Paternak JJ (1986) Physiological effects of plasmid DNA transformation onAzotobacter vinelandii. Can J Microbiol 32:145–148
Glick BR, Pasternak JJ, Brooks HE (1987) The development ofAzotobacter as a bacterial fertilizer by the introduction of exogenous cellulase genes. In: Hasnain S, Lamptey J, Moo-Young M (eds) Biotechnology and renewable energy. Amsterdam: Elsevier, pp 125–134
Gordon JK, Jacobsen MR (1983) Isolation and characterization ofAzotobacter vinelandii mutant strains with potential as bacterial fertilizer. Can J Microbiol 29:973–978
Lem NW, Glick BR (1985) Biotechnological uses of cyanobacteria. Biotechnol Adv 3:195–208
Lifshitz R, Kloepper JW, Kozlowski M, Simonson C, Carlson J, Tipping EM, Zaleska I (1987) Growth promotion of canola (rapessed) by a strain ofPseudomonas putida under gnotobiotic conditions. Can J Microbiol 33:390–395
Maia M, Vela GR (1987) Plasmids ofAzotobacter vinelandii. Am Soc Microbiol 89th Annual Mtg., abstract #H-54
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory
Okon Y (1985)Azospirillum as a potential inoculant for agriculture. Trends Biotechnol 3:223–228
Sprent JI (1986) Benefits ofRhizobium to agriculture. Trends Biotechnol 4:124–129
Steele BF, Sauberlich HE, Reynolds MS, Baumann CA (1949) Media forLeuconostoc mesenteroides P-60 andLeuconostoc citrovorum 8081. J Biol Chem 177:533–542
Whitney GK, Glick BR, Robinson CW (1989) Induction of T4 DNA ligase in a recombinant strain ofEscherichia coli. Biotech. Bioeng. 33:991–998
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Glick, B.R., Butler, B.J., Mayfield, C.I. et al. Effect of transformation ofAzotobacter vinelandii with the low copy number plasmid pRK290. Current Microbiology 19, 143–146 (1989). https://doi.org/10.1007/BF01568932
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DOI: https://doi.org/10.1007/BF01568932