Abstract
Strain dependent differences were found in the ability of inbred mice to produce antibodies to a thymus independent synthetic polypeptide, poly (DTyr, DGlu)-polyDPro-polyDLys. These differences were detected after injecting the antigen either in complete Freund's adjuvant or in aqueous solution, and were significant already in the primary response. High- and low-responder mice produced mainly antibodies of the IgG class as deduced from their mercaptoethanol resistance and their elution in the second fraction of a Sephadex G-200 chromatography. Genetic analysis of the immune response potential to poly(DTyr,DGlu)-polyDPro-polyDLys has indicated that responsiveness to this immunogen is controlled by a dominant, quantitative gene(s) which is not linked to either the major histocompatibility complex (H-2) of the mouse or to the heavy chain locus and is not located on the X-chromosome.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allison, A. and Davies, A.: Requirement of thymus dependent lymphocytes for potentiation by adjuvants of antibody formation.Nature 233:330–332, 1971.
Amsbaugh, D., Hansen, C., Prescott, B., Stashak, P., Barthold, D., and Baker, P.: Genetic control of the antibody response to type III pneumococcal polysaccharide in mice.J. Exp. Med. 136:931–947, 1972.
Andersson, B.: Some characteristics of mouse antibodies to polyvinylpyrrolidone. Thesis — Tryckeri Balder AB., Stockholm 1972, Chapter 2.
Barthold, D., Stashak, P., Amsbaugh, D., Prescott, B., and Baker, P.: Strain differences in the ability of antithymocytes serum (ATS) to enhance the antibody response of inbred mice to type III pneumococcal polysaccharide.Cell. Immunol. 6:315–323, 1973.
Benacerraf, B. and McDevitt, H. O.: Histocompatibility-linked immune response genes.Science 175:273–279, 1972.
Blomberg, B., Geckeler, W., and Weigert, M.: Genetics of the antibody response to dextran in mice.Science 177:178–180, 1972.
Britton, S. and Möller, G.: Regulation of antibody synthesis againstEscherichia coli endotoxin. I. Suppressive effect of endogenously produced and passively transferred antibodies.J. Immunol. 100:1326–1333, 1968.
Del Guercio, P. and Leuchars, E.: The immune response in mice to the haptenic determinant DNP coupled to a thymus-independent carrier (levan).J. Immunol. 109:951–956, 1972.
Gasser, D. L.: Genetic control of the immune response in mice. I. Segregation data and localization to the fifth linkage group of a gene affecting antibody production.J. Immunol. 103:66–70, 1969.
Grumet, F. C.: Genetic control of the immune response. A selective defect in immuno logic (IgG) memory in nonresponder mice.J. Exp. Med. 135:110–125, 1972.
Grumet, F. C., Mitchell, G. F., and McDevitt, H. O.: Genetic control of the immune response: The effect of thymectomy on the primary and secondary antibody response of mice to (T,G)-A-L.J. Exp. Med. 135:126–135, 1972.
Herbert, W. J.: Passive haemagglutination with special reference to the tanned cell technique.In D. M. Weir (ed.):Handbook of Experimental Immunology; 20.1–20.20, Blackwell, Oxford, 1973.
Herzenberg, L. A., McDevitt, H. O., Herzenberg, L. A.: Genetics of antibodies.Ann. Rev. Genet. 2:209–244, 1968.
Howard, J. G., Christie, G. H., and Courtenay, B. M.: Studies on immunological paralysis. IV. The relative contributions of continuous antibody neutralisation and central inhibitor to paralysis with type III pneumococcal polysaccharide.Proc. Roy. Soc. Lond. B. 178:417–438, 1971.
Ishizaka, K., Ishizaka, T., Lee, E., and Fudenberg, H.: Immunochemical properties of human gamma A isohemagglutinin. I. Comparisons withγG- andγM-globulin antibodies.J. Immunol. 95:197–208, 1965.
Jaton, J. -C. and Sela, M.: Role of optical configuration in the immunogenicity and specificity of synthetic antigens derived from multichain polyproline.J. Biol. Chem. 243:5616–5626, 1968.
Lichtenberg, L. A., Shearer, G. M., Mozes, E., and Sela, M. The role of thymus cells in the immune response to poly(Tyr,Glu)-poly DLAla-polyLys as a function of the genetic constitution of the mouse strain.Eur. J. Immunol. 4:430–434, 1974.
McDevitt, H. O.: Genetic control of the antibody response. III. Qualitative and quantitative characterization of the antibody response to (T,G)-A-L in CBA and C57 mice.J. Immunol. 100:485–492, 1968.
McDevitt, H. O. and Benacerraf, B.: Genetic control of specific immune responses.Adv. Immunol. 11:31–74, 1969.
Miranda, J. J.: Studies on immunological paralysis IX. The immunogenicity and tolerogenicity of levan (polyfructose) in mice.Immunology 23:829–842, 1972.
Mozes, E., McDevitt, H. O., Jaton, J. -C., and Sela, M.: The nature of the antigenic determinant in a genetic control of the antibody response.J. Exp. Med. 130:493–503, 1969a.
Mozes, E., McDevitt, H. O., Jaton, J. -C., and Sela, M.: The genetic control of antibody specificity.J. Exp. Med. 130:1263–1278, 1969b.
Mozes, E. and Shearer, G.: Contribution of bone marrow cells and lack of expression of thymocytes in genetic controls of immune responses for two immunopotent regions within poly-(Phe,Glu)-PolyPro-polyLys in inbred mouse strains.J. Exp. Med. 134:141–161, 1971.
Mozes, E. and Shearer, G. M.: Genetic control of immune responses. Curr. Topics Microbiol. Immunol.59:167–200, 1972.
Mozes, E., Sela, M., and McDevitt, H. O.: Genetic control of immune response in mice to derivatives of multichain polyproline differing in the optical configuration of component amino acids.Eur. J. Immunol. 3:1–6, 1973.
Mozes, E. and Fuchs, S.: Linkage between the immune response potential to DNA and the X-chromosome.Nature 249:167–168, 1974.
Mozes, E. and Sela, M.: The role of the thymus in a genetically controlled defect of the immune response at the carrier level.Proc. Nat. Acad. Sci. USA,71:1574–1577, 1974.
Ouchterlony, Ö.: Antigen-antibody reaction in gels. IV. Types of reactions in coordinated systems of diffusion.Acta Pathol. Microbiol. Scand. 32:231–240, 1953.
Scher, I., Frantz, M. M., and Steinberg, A. D.: The genetics of the immune responses to a synthetic double-stranded RNA in a mutant CBA mouse strain.J. Immunol. 110:1396–1401, 1973.
Sela, M., Mozes, E., and Shearer, G. M.: Thymus-independence of slowly metabolized immunogens.Proc. Nat. Acad. Sci. USA69:2696–2700, 1972.
Shearer, G. M., Mozes, E., and Sela, M.: Contribution of different cell types to the genetic control of immune response as a function of the chemical nature of the polymeric side chain (poly-L-prolyl and poly-DL-alanyl) of synthetic immunogens.J. Exp. Med. 135:1009–1027, 1972.
Stimpfling, J. H.: The use of PVP as a developing agent in mouse hemagglutination tests.Transplant. Bull. 27:109–111, 1961.
Stimpfling, J. H., Boyse, E. A., and Mishell, R.: II. Preparation of isoantisera in laboratory mice.Meth. Med. Res. 10:18–21, 1964.
Stollar, B. D., Fuchs, S., Mozes, E.: Immune response of mice to nucleic acids: strain-dependent differences in magnitude and class of antibody production.J. Immunol. 111:121–129, 1973.
Weiler, E., Melletz, E., Breuninger-Peck, E.: Facilitation of immune hemolysis by an interaction between red cell-sensitizing antibody and gamma-globulin allotype antibody.Proc. Nat. Acad. Sci. USA54:1310–1317, 1965.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schmitt-Verhulst, A.M., Mozes, E. & Sela, M. Genetic control of the immune response to a thymus independent synthetic polypeptide. Immunogenetics 1, 357–369 (1974). https://doi.org/10.1007/BF01564074
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01564074