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Mutant alleles for hypoxanthine phosphoribosyltransferase: Codominant expression, complementation, and segregation in hybrid Chinese hamster cells

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Somatic Cell Genetics

Abstract

Chinese hamster ovary cell mutants resistant to the purine analogs 6-thioguanine or 8-azaguanine have been isolated following mutagenesis with ethyl methane sulfonate. The activities of hypoxanthine phosphoribosyl-transferase (HPRT) in three such mutants have been found to exhibit an increased K m for the substrate 5-phosphoribosyl-1-pyrophosphate. The isoelectric point of the mutant enzyme activity has also changed in two mutants. Hybrid cells containing one mutant and one wild-type allele express both genes. Segregants that have lost only the wild-type allele can be selected on the basis of drug resistance. Two mutants exhibiting different alterations in HPRT activity can complement in a hybrid cell to yield a wild-type growth pattern and enzyme activity with intermediate electrophoretic and kinetic properties. The results suggest intracistronic complementation between structural gene mutants of HPRT.

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Authors and Affiliations

  1. Department of Biological Sciences, Columbia University, 10027, New York, New York

    L. A. Chasin & G. Urlaub

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  1. L. A. Chasin
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  2. G. Urlaub
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Chasin, L.A., Urlaub, G. Mutant alleles for hypoxanthine phosphoribosyltransferase: Codominant expression, complementation, and segregation in hybrid Chinese hamster cells. Somat Cell Mol Genet 2, 453–467 (1976). https://doi.org/10.1007/BF01542725

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  • DOI: https://doi.org/10.1007/BF01542725

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