Skip to main content
SpringerLink
Log in

Use of muscovy duck embryo fibroblasts for the isolation of viruses from wild birds

  • Published:
Journal of tissue culture methods

Summary

Techniques are described for the preparation, cryopreservation, and inoculation of Muscovy duck embryo cell cultures. The procedure yields a susceptible reproducible cell culture system for the isolation and cultivation of viruses from wild birds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Burgess, E. C.; Yuill, T. M. Increased cell culture incubation temperatures for duck plaque virus isolation. Avian Dis. 25:222–224; 1981.

    PubMed  Google Scholar 

  2. Dein, F. J.; Carpenter, J. W.; Clark, G. G., et al. Mortality of captive whooping cranes caused by eastern equine encephalitis virus. J. Am. Vet. Med. Assoc. 189:1006–1010; 1986.

    PubMed  Google Scholar 

  3. Docherty, D. E.; Henning, D. J. Isolation of a herpesvirus from captive cranes with an inclusion body disease. Avian Dis. 24:278–283; 1980.

    Google Scholar 

  4. Docherty, D. E.; Long, R. I. R. Isolation of a poxvirus from a house finch,Carpodadcus mexicanus. J. Wildl. Dis. 22:420–422; 1986.

    PubMed  Google Scholar 

  5. Docherty, D. E.; Romaine, R. I. Inclusion body disease of cranes: a serological follow-up to the 1978 die-off. Avian Dis. 27:830–835; 1983.

    PubMed  Google Scholar 

  6. Feldman, H.; Wang, S. Sensitivity of various viruses to chloroform. Proc. Soc. Exp. Biol. Med. 106:736–738; 1961.

    PubMed  Google Scholar 

  7. Freshney, R. I. Disaggregation of the tissue and primary culture. In: Culture of animal cells, a manual of basic technique. New York: Alan R. Liss; 1983:99–118.

    Google Scholar 

  8. Hunter, E. Biological techniques for avian sarcoma viruses. In: Jackoby, W. B.; Pastan, I. H., eds. Methods of enzymology, vol. LVIII. Cell culture. New York: Academic Press; 1979:379–393.

    Google Scholar 

  9. Kocan, R. M. Duck plaque virus replication in Muscovy duck fibroblast cells. Avian Dis. 20:574–580; 1976.

    PubMed  Google Scholar 

  10. McManus, A. T.; Parker, D. R.; Kenyon, R. H., et al. Use of frozen chick and duck embryo cells for plaque assays of arboviruses and rickettsiae. J. Biol. Stand. 2:329–332; 1974.

    PubMed  Google Scholar 

  11. Rovozzo, G. C.; Burke, C. N. Tissue culture propagation. In: A manual of basic virological techniques. Englewood Cliffs, NJ: Prentice-Hall, Inc.; 1973:38–63, 150–151.

    Google Scholar 

  12. Tokumaru, T. Herpesviruses. In: Lennette, E. H.; Schmidt, N. J., eds. Diagnostic procedures for viral and rickettsial infections, 4th ed. New York: American Public Health Association. 1969:660.

    Google Scholar 

  13. Vogt, P. K. Focus assay of Rous sarcoma virus. In: Habel, K.; Saltzman, N. P., eds. Fundamental techniques in virology. New York: Academic Press; 1977:198–211.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fish and Wildlife Service, U.S. Dept. of Interior, National Wildlife Health Research Center, 53711, Madison, Wisconsin

    Douglas E. Docherty & Paul G. Slota

Authors
  1. Douglas E. Docherty
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paul G. Slota
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Docherty, D.E., Slota, P.G. Use of muscovy duck embryo fibroblasts for the isolation of viruses from wild birds. Journal of Tissue Culture Methods 11, 165–170 (1988). https://doi.org/10.1007/BF01404270

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01404270

Key words

Search

Navigation