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Differentiation of vaccine and wild-type polioviruses using polymerase chain reaction and restriction enzyme analysis

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Summary

Genomic amplification followed by selective digestion of restriction enzymes was used to differentiate polioviruses. The method was based on conserved and variable components of the 5′-noncoding region. The differences between Sabin vaccine and wild-type viruses made it possible to identify rapidly an isolated poliovirus as vaccine-related or wild-type virus. A total of 60 isolates and strains were tested and all of them were correctly identified. This method is recommended as a sensitive, specific and rapid way to differentiate polioviruses in clinical isolates and environmental samples.

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Authors and Affiliations

  1. Robert Koch Institute of the Federal Health Office, Berlin, Federal Republic of Germany

    B. Schweiger, E. Schreier & B. Böthig

  2. Institute for Water, Soil, and Air Hygiene of the Federal Health Office, Berlin, Federal Republic of Germany

    J. M. López-Pila

Authors
  1. B. Schweiger
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  2. E. Schreier
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  3. B. Böthig
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  4. J. M. López-Pila
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Schweiger, B., Schreier, E., Böthig, B. et al. Differentiation of vaccine and wild-type polioviruses using polymerase chain reaction and restriction enzyme analysis. Archives of Virology 134, 39–50 (1994). https://doi.org/10.1007/BF01379105

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  • DOI: https://doi.org/10.1007/BF01379105

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