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Biophysical properties of virions of human adenovirus of the type 6 and its DNA

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Summary

A study has been made of human adenovirus type 6 (Ad 6) which belongs to the C subgroup of the nononcogenic adenoviruses. The buoyant density of the Ad 6 virions in CsCl gradient was 1.3545±0.0010 g/cm3. The DNA content determined from the buoyant density values and direct phosphorus analysis was 11.2±0.5 and 11.5±0.5 per cent, respectively. The S 020,w and D 020,w values for the Ad 6 virions were 795±18S and (3.255±0.035)×10−8 cm2/s. The hydrodynamic parameters of the viral particle and its volume as well as the specific partial volume, the content and the molecular weight of the DNA have yielded a molecular weight of the virions of (203.6±10.0)×106.

The sedimentation constant and the intrinsic viscosity of the DNA have been found to be 32.7±0.7S and 93.5±5.0 dl/g, respectively. The molecular weight of the DNA found from these results is (23.4±0.8)×106.

The buoyant density of the Ad 6 DNA measured in CsCl and Cs2SO4 density gradients was 1.7128±0.0010 and 1.427±0.001 g/cm3, respectively. The GC content found from the buoyant density and the melting temperature (90.5±0.1° C in 1 SSC and 75.0±0.1° C in 0.1 SSC) was 52.6±1.0 per cent, that is, it is considerably lower than the GC content of 56–60 per cent which, according to the “Green's rule”, should be typical of the DNAs of all the human adenoviruses of the subgroup C (30).

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Authors and Affiliations

  1. D. I. Ivanovsky Institute of Virology of the Academy of Medical Sciences of the U.S.S.R., Moscow, USSR

    T. I. Tikchonenko, A. G. Dubichev, N. N. Parfenov & N. M. Chaplygina

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  1. T. I. Tikchonenko
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  2. A. G. Dubichev
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  3. N. N. Parfenov
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  4. N. M. Chaplygina
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  5. R. S. Dreizin
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  6. E. E. Zolotarskaya
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Tikchonenko, T.I., Dubichev, A.G., Parfenov, N.N. et al. Biophysical properties of virions of human adenovirus of the type 6 and its DNA. Archives of Virology 62, 117–130 (1979). https://doi.org/10.1007/BF01318064

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